KEGG   ENZYME: 2.4.1.212Help
Entry
EC 2.4.1.212                Enzyme                                 

Name
hyaluronan synthase;
spHAS;
seHAS
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
Alternating UDP-alpha-N-acetyl-D-glucosamine:beta-D-glucuronosyl-(1->3)-[nascent hyaluronan] 4-N-acetyl-beta-D-glucosaminyltransferase and UDP-alpha-D-glucuronate:N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] 3-beta-D-glucuronosyltransferase
Reaction(IUBMB)
(1) UDP-alpha-N-acetyl-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] [RN:R05327 R06068];
(2) UDP-alpha-D-glucuronate + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-[nascent hyaluronan] = UDP + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-[nascent hyaluronan]
Reaction(KEGG)
Substrate
UDP-alpha-N-acetyl-D-glucosamine;
beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan];
UDP-alpha-D-glucuronate [CPD:C00167];
N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-[nascent hyaluronan]
Product
UDP [CPD:C00015];
N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan];
beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-[nascent hyaluronan]
Comment
The enzyme from Streptococcus Group A and Group C requires Mg2+. The enzyme adds GlcNAc to nascent hyaluronan when the non-reducing end is GlcA, but it adds GlcA when the non-reducing end is GlcNAc [3]. The enzyme is highly specific for UDP-GlcNAc and UDP-GlcA; no copolymerization is observed if either is replaced by UDP-Glc, UDP-Gal, UDP-GalNAc or UDP-GalA. Similar enzymes have been found in a variety of organisms.
History
EC 2.4.1.212 created 2001, modified 2007
Orthology
K00752  
hyaluronan synthase
Genes
HSA: 
3036(HAS1) 3037(HAS2) 3038(HAS3)
PTR: 
454195(HAS3) 468978(HAS1) 472850(HAS2)
PPS: 
100974629(HAS1) 100978574(HAS3) 100988254(HAS2)
GGO: 
101133545(HAS1) 101146377(HAS3)
PON: 
MCC: 
703353(HAS2) 704121(HAS3) 719773(HAS1)
MCF: 
102114972(HAS3) 102131327(HAS2) 102135282(HAS1)
MMU: 
15116(Has1) 15117(Has2) 15118(Has3)
RNO: 
25694(Has2) 266805(Has3) 282821(Has1)
CGE: 
100751055(Has2) 100757895(Has3)
HGL: 
101707808(Has1) 101717946(Has3) 101724130(Has2)
TUP: 
102483298(HAS1) 102489956(HAS3) 102491778(HAS2)
CFA: 
482032(HAS2) 489739(HAS3) 611700(HAS1)
AML: 
FCA: 
101093224(HAS1) 101096124(HAS3) 101099432(HAS2)
PTG: 
102950235(HAS2) 102953815(HAS3) 102955943(HAS1)
BTA: 
281220(HAS2) 282820(HAS1) 286824(HAS3)
BOM: 
PHD: 
CHX: 
102170334(HAS2) 102170999(HAS3) 102180619(HAS1)
SSC: 
CFR: 
BACU: 
103000737(HAS3) 103019394(HAS1)
LVE: 
103072634(HAS3) 103077212(HAS1) 103090515(HAS2)
ECB: 
100009708(HAS2) 100066947(HAS3)
MYB: 
MYD: 
PALE: 
102879345(HAS2) 102887158(HAS3) 102894200(HAS1)
MDO: 
100020005(HAS3) 100025815(HAS2) 100028664(HAS1)
SHR: 
OAA: 
100073390(HAS2) 100077175(HAS3) 100088892(HAS1)
GGA: 
395594(HAS2) 427564(HAS3)
MGP: 
TGU: 
100228837(HAS2)
FAB: 
101817917(HAS2) 101822065(HAS3)
PHI: 
102104183(HAS3) 102113165(HAS2)
APLA: 
101794398(HAS3) 101798379(HAS2)
FPG: 
101913427(HAS3) 101916039(HAS2)
FCH: 
102049447(HAS3) 102052598(HAS2)
CLV: 
102086971(HAS3) 102087835(HAS2)
ASN: 
102373237(HAS3) 102374679(HAS1) 102388766(HAS2)
AMJ: 
PSS: 
102445131(HAS2)
CMY: 
ACS: 
PBI: 
XLA: 
379383(has1) 399037(has3) 399146(has2)
XTR: 
DRE: 
260350(has2) 282555(has3) 403130(has1)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
CMK: 
BFO: 
PAN: 
MTM: 
TRE: 
SSL: 
BFU: 
MBE: 
AOR: 
AOR_1_618174(AO090005000353)
AFV: 
PNO: 
ZTR: 
PFJ: 
PPL: 
DMA: 
DAS: 
DAO: 
PHL: 
BAH: 
BAX: 
BANT: 
BANR: 
BAL: 
BCX: 
LSP: 
LGY: 
BBE: 
GYM: 
SPY: 
SPy_2200(hasA)
SPZ: 
SPYM: 
SPM: 
SPG: 
SPS: 
SPH: 
SPJ: 
SPK: 
SPF: 
SpyM51824(hasA)
SPA: 
SPB: 
STG: 
SOZ: 
STZ: 
STX: 
SPYA: 
A20_1895(hasA)
SPYH: 
SNX: 
SNU: 
SPNE: 
SPNU: 
SPNM: 
SPNO: 
STE: 
STN: 
SEQ: 
SEZ: 
Sez_0199(hasA)
SEZO: 
SEU: 
SUB: 
SUB1697(hasA)
STK: 
STP_1716(hasA)
SIK: 
EFN: 
CPY: 
CAD: 
SSG: 
SRI: 
AIN: 
MSM: 
MSG: 
MSA: 
MCB: 
SDV: 
KSK: 
LXY: 
MTS: 
BCV: 
CFL: 
CFI: 
FRE: 
FSY: 
ACTN: 
AFS: 
CWO: 
GFO: 
GFO_0015(hasA)
FJO: 
CAO: 
CLY: 
KDI: 
LAN: 
PTQ: 
FBA: 
STO: 
NKR: 
NGA: 
 » show all
Taxonomy
Reference
1  [PMID:8366070]
  Authors
DeAngelis PL, Papaconstantinou J, Weigel PH.
  Title
Molecular cloning, identification, and sequence of the hyaluronan synthase gene from group A Streptococcus pyogenes.
  Journal
J. Biol. Chem. 268 (1993) 19181-4.
  Organism
Streptococcus pyogenes
  Sequence
Reference
2  [PMID:10988250]
  Authors
Jing W, DeAngelis PL.
  Title
Dissection of the two transferase activities of the Pasteurella multocida hyaluronan synthase: two active sites exist in one polypeptide.
  Journal
Glycobiology. 10 (2000) 883-9.
  Organism
Pasteurella multocida
  Sequence
[pmp:Pmu_08530]
Reference
3  [PMID:10473619]
  Authors
DeAngelis PL.
  Title
Molecular directionality of polysaccharide polymerization by the Pasteurella multocida hyaluronan synthase.
  Journal
J. Biol. Chem. 274 (1999) 26557-62.
  Organism
Pasteurella multocida
Reference
4  [PMID:15248781]
  Authors
Tlapak-Simmons VL, Baron CA, Weigel PH.
  Title
Characterization of the purified hyaluronan synthase from Streptococcus equisimilis.
  Journal
Biochemistry. 43 (2004) 9234-42.
  Organism
Streptococcus equisimilis
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
39346-43-5

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