6G-fructosyltransferase;
fructan:fructan 6G-fructosyltransferase;
1F(1-beta-D-fructofuranosyl)m sucrose:1F(1-beta-D-fructofuranosyl)nsucrose 6G-fructosyltransferase;
6G-FFT;
6G-FT;
6G-fructotransferase

Transferases;
Glycosyltransferases;
Hexosyltransferases

1F-oligo[beta-D-fructofuranosyl-(2->1)-]sucrose 6G-beta-D-fructotransferase

[1-beta-D-fructofuranosyl-(2->1)-]m+1 alpha-D-glucopyranoside + [1-beta-D-fructofuranosyl-(2->1)-]n alpha-D-glucopyranoside = [1-beta-D-fructofuranosyl-(2->1)-]m alpha-D-glucopyranoside + [1-beta-D-fructofuranosyl-(2->1)-]n beta-D-fructofuranosyl-(2->6)-alpha-D-glucopyranoside (m > 0; n >= 0)

[1-beta-D-fructofuranosyl-(2->1)-]m+1 alpha-D-glucopyranoside;
[1-beta-D-fructofuranosyl-(2->1)-]n alpha-D-glucopyranoside

This enzyme catalyses the transfer of the terminal (2->1)-linked beta-D-fructosyl group of a mono- or oligosaccharide substituent on O-1 of the fructose residue of sucrose onto O-6 of its glucose residue [1]. For example, if 1-kestose [1F-(beta-D-fructofuranosyl)sucrose] is both the donor and recipient in the reaction shown above, i.e., if m = 1 and n = 1, then the products will be sucrose and 6G-di-beta-D-fructofuranosylsucrose. In this notation, the superscripts F and G are used to specify whether the fructose or glucose residue of the sucrose carries the substituent. Alternatively, this may be indicated by the presence and/or absence of primes (see http://www.chem.qmul.ac.uk/iupac/2carb/36.html#362). Sucrose cannot be a donor substrate in the reaction (i.e. m cannot be zero) and inulin cannot act as an acceptor. Side reactions catalysed are transfer of a beta-D-fructosyl group between compounds of the structure 1F-(1-beta-D-fructofuranosyl)m-6G-(1-beta-D-fructofuranosyl)n sucrose, where m >= 0 and n = 1 for the donor, and m >= 0 and n >= 0 for the acceptor.

1

Shiomi, N.

Purification and characterisation of 6G-fructosyltransferase from the roots of asparagus (Asparagus officinalis L.).

Carbohydr. Res. 96 (1981) 281-292.

2

Shiomi, N.

Reverse reaction of fructosyl transfer catalysed by asparagus 6G-fructosyltransferase.

Carbohydr. Res. 106 (1982) 166-169.

3

Shiomi, N. and Ueno, K.

Cloning and expression of genes encoding fructosyltransferases from higher plants in food technology.

J. Appl. Glycosci. 51 (2004) 177-183.

4  [PMID:15720693]

Ueno K, Onodera S, Kawakami A, Yoshida M, Shiomi N.

Molecular characterization and expression of a cDNA encoding fructan:fructan 6G-fructosyltransferase from asparagus (Asparagus officinalis).

New. Phytol. 165 (2005) 813-24.

Asparagus officinalis