KEGG   ENZYME: 2.4.1.258Help
Entry
EC 2.4.1.258                Enzyme                                 

Name
dolichyl-P-Man:Man5GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase;
Man5GlcNAc2-PP-Dol mannosyltransferase;
ALG3;
dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase;
Not56-like protein;
Alg3 alpha-1,3-mannosyl transferase;
Dol-P-Man:Man5GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
dolichyl beta-D-mannosyl phosphate:D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,3-mannosyltransferase
Reaction(IUBMB)
dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate [RN:R06258]
Reaction(KEGG)
R06258(G)
Show
Substrate
dolichyl beta-D-mannosyl phosphate;
D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
Product
D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol;
dolichyl phosphate [CPD:C00110]
Comment
The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl diphosphate. Early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-dolichol to Man9Glc-NAc2-PP-dolichol on the lumenal side use dolichyl beta-D-mannosyl phosphate. The first step of this assembly pathway on the luminal side of the endoplasmic reticulum is catalysed by ALG3.
Pathway
N-Glycan biosynthesis
Various types of N-glycan biosynthesis
Metabolic pathways
Orthology
K03845  
alpha-1,3-mannosyltransferase
Genes
HSA: 
10195(ALG3)
PTR: 
460886(ALG3)
PPS: 
100979287(ALG3)
GGO: 
101148766(ALG3)
PON: 
100448751(ALG3)
MCC: 
703087(ALG3)
MCF: 
102134126(ALG3)
MMU: 
208624(Alg3)
RNO: 
287983(Alg3)
CGE: 
100772003(Alg3)
HGL: 
101714764(Alg3)
TUP: 
102473222(ALG3)
CFA: 
478653(ALG3)
AML: 
FCA: 
101087681(ALG3)
BTA: 
614624(ALG3)
BOM: 
102285313(ALG3)
PHD: 
102320266(ALG3)
CHX: 
102181899(ALG3)
SSC: 
100627022(ALG3)
CFR: 
102504086(ALG3)
ECB: 
100059007(ALG3)
MYB: 
102241054(ALG3)
MDO: 
SHR: 
100932630(ALG3)
OAA: 
GGA: 
424952(ALG3)
MGP: 
TGU: 
100230731(ALG3)
FAB: 
101819823(ALG3)
PHI: 
102102878(ALG3)
APLA: 
101791055(ALG3)
FPG: 
101916756(ALG3)
FCH: 
102050162(ALG3)
CLV: 
102094667(ALG3)
ASN: 
102369785(ALG3)
PSS: 
102457770(ALG3)
ACS: 
XLA: 
100037243(alg3)
XTR: 
100127746(alg3)
DRE: 
553725(alg3)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
102361691(ALG3)
BFO: 
CIN: 
SPU: 
DME: 
Dmel_CG4084(l(2)not)
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
Dvir_GJ18303(Dvir_l(2)not)
AGA: 
AAG: 
CQU: 
AME: 
724399(GB12491)
NVI: 
TCA: 
PHU: 
ISC: 
CBR: 
LOA: 
TSP: 
SMM: 
NVE: 
HMG: 
AQU: 
ATH: 
AT2G47760(ALG3)
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
GMX: 
MTR: 
CAM: 
FVE: 
CSV: 
RCU: 
POP: 
POPTR_0014s12610g(POPTRDRAFT_731888)
VVI: 
SLY: 
SOT: 
DOSA: 
Os01t0172000-01(Os01g0172000)
BDI: 
SBI: 
SORBI_03g004440(SORBIDRAFT_03g004440)
ZMA: 
SITA: 
SMO: 
PPP: 
OLU: 
MIS: 
MPP: 
CSL: 
CME: 
GSL: 
CCP: 
SCE: 
YBL082C(ALG3)
AGO: 
ERC: 
KLA: 
LTH: 
PPA: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0E02510(NCAS0E02510)
NDI: 
NDAI_0E04080(NDAI0E04080)
TPF: 
TPHA_0K00760(TPHA0K00760)
TBL: 
TBLA_0D00510(TBLA0D00510)
TDL: 
TDEL_0C02670(TDEL0C02670)
KAF: 
KAFR_0L01850(KAFR0L01850)
DHA: 
PIC: 
PGU: 
LEL: 
CAL: 
CTP: 
CDU: 
COT: 
YLI: 
CLU: 
NCR: 
SMP: 
PAN: 
TTT: 
MTM: 
MGR: 
FGR: 
NHE: 
VAL: 
SSL: 
BFU: 
ANI: 
NFI: 
AFM: 
AOR: 
AOR_1_556094(AO090120000319)
ACT: 
PCS: 
CIM: 
CPW: 
PBL: 
URE: 
ABE: 
TVE: 
AJE: 
PTE: 
ZTR: 
TML: 
SPO: 
CNE: 
CNB: 
CGI: 
LBC: 
MPR: 
CCI: 
SCM: 
PGR: 
MBR: 
DDI: 
DPP: 
DFA: 
DFA_10860(alg3)
ACAN: 
PTI: 
TPS: 
EHX: 
GTT: 
TBR: 
TCR: 
LMA: 
LIF: 
LDO: 
LMI: 
LBZ: 
 » show all
Taxonomy
Reference
1  [PMID:11308030]
  Authors
Sharma CB, Knauer R, Lehle L
  Title
Biosynthesis of lipid-linked oligosaccharides in yeast: the ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.
  Journal
Biol. Chem. 382 (2001) 321-8.
  Organism
Saccharomyces cerevisiae [GN:sce]
  Sequence
[sce:YBL082C]
Reference
2  [PMID:10660594]
  Authors
Cipollo JF, Trimble RB
  Title
The accumulation of Man(6)GlcNAc(2)-PP-dolichol in the Saccharomyces cerevisiae Deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm  addition in downstream oligosaccharide-lipid and glycoprotein glycan processing.
  Journal
J. Biol. Chem. 275 (2000) 4267-77.
  Organism
Saccharomyces cerevisiae [GN:sce]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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