KEGG   ENZYME: 2.4.1.258Help
Entry
EC 2.4.1.258                Enzyme                                 

Name
dolichyl-P-Man:Man5GlcNAc2-PP-dolichol alpha-1,3-mannosyltransferase;
Man5GlcNAc2-PP-Dol mannosyltransferase;
ALG3;
dolichyl-P-Man:Man(5)GlcNAc(2)-PP-dolichyl mannosyltransferase;
Not56-like protein;
Alg3 alpha-1,3-mannosyl transferase;
Dol-P-Man:Man5GlcNAc2-PP-Dol alpha-1,3-mannosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
dolichyl beta-D-mannosyl phosphate:D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,3-mannosyltransferase
Reaction(IUBMB)
dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate [RN:R06258]
Reaction(KEGG)
R06258(G)
Show
Substrate
dolichyl beta-D-mannosyl phosphate;
D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
Product
D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->3)-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol;
dolichyl phosphate [CPD:C00110]
Comment
The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl diphosphate. Early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-dolichol to Man9Glc-NAc2-PP-dolichol on the lumenal side use dolichyl beta-D-mannosyl phosphate. The first step of this assembly pathway on the luminal side of the endoplasmic reticulum is catalysed by ALG3.
History
EC 2.4.1.258 created 1976 as EC 2.4.1.130, part transferred 2011 to EC 2.4.1.258, modified 2012
Pathway
N-Glycan biosynthesis
Various types of N-glycan biosynthesis
Metabolic pathways
Orthology
K03845  
alpha-1,3-mannosyltransferase
Genes
HSA: 
10195(ALG3)
PTR: 
460886(ALG3)
PPS: 
100979287(ALG3)
GGO: 
101148766(ALG3)
PON: 
100448751(ALG3)
MCC: 
703087(ALG3)
MCF: 
102134126(ALG3)
MMU: 
208624(Alg3)
RNO: 
287983(Alg3)
CGE: 
100772003(Alg3)
HGL: 
101714764(Alg3)
TUP: 
102473222(ALG3)
CFA: 
478653(ALG3)
AML: 
FCA: 
101087681(ALG3)
PTG: 
102971503(ALG3)
BTA: 
614624(ALG3)
BOM: 
102285313(ALG3)
PHD: 
102320266(ALG3)
CHX: 
102181899(ALG3)
OAS: 
101121650(ALG3)
SSC: 
100627022(ALG3)
CFR: 
102504086(ALG3)
BACU: 
103005524(ALG3)
LVE: 
103084099(ALG3)
ECB: 
100059007(ALG3)
MYB: 
102241054(ALG3)
MYD: 
102766472(ALG3)
PALE: 
102890134(ALG3)
MDO: 
100025876(ALG3)
SHR: 
100932630(ALG3)
OAA: 
GGA: 
424952(ALG3)
MGP: 
TGU: 
100230731(ALG3)
FAB: 
101819823(ALG3)
PHI: 
102102878(ALG3)
APLA: 
101791055(ALG3)
FPG: 
101916756(ALG3)
FCH: 
102050162(ALG3)
CLV: 
102094667(ALG3)
ASN: 
102369785(ALG3)
AMJ: 
102560066(ALG3)
PSS: 
102457770(ALG3)
CMY: 
102937300(ALG3)
ACS: 
100561775(alg3)
PBI: 
103048200(ALG3)
XLA: 
100037243(alg3)
XTR: 
100127746(alg3)
DRE: 
553725(alg3)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
102361691(ALG3)
CMK: 
103182887(alg3)
BFO: 
CIN: 
SPU: 
DME: 
Dmel_CG4084(l(2)not)
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
Dvir_GJ18303(Dvir_l(2)not)
AGA: 
AAG: 
CQU: 
AME: 
724399(GB12491)
NVI: 
TCA: 
PHU: 
ISC: 
CBR: 
LOA: 
TSP: 
SMM: 
NVE: 
HMG: 
AQU: 
ATH: 
AT2G47760(ALG3)
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
TCC: 
GMX: 
PVU: 
MTR: 
CAM: 
FVE: 
PPER: 
PMUM: 
MDM: 
CSV: 
CMO: 
RCU: 
POP: 
POPTR_0014s12610g(POPTRDRAFT_731888)
VVI: 
SLY: 
SOT: 
DOSA: 
Os01t0172000-01(Os01g0172000)
OBR: 
BDI: 
SBI: 
SORBI_03g004440(SORBIDRAFT_03g004440)
ZMA: 
SITA: 
ATR: 
s00045p00202440(AMTR_s00045p00202440)
SMO: 
PPP: 
OLU: 
BPG: 
MIS: 
MPP: 
CSL: 
CME: 
GSL: 
CCP: 
SCE: 
YBL082C(ALG3)
AGO: 
ERC: 
KLA: 
LTH: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0E02510(NCAS0E02510)
NDI: 
NDAI_0E04080(NDAI0E04080)
TPF: 
TPHA_0K00760(TPHA0K00760)
TBL: 
TBLA_0D00510(TBLA0D00510)
TDL: 
TDEL_0C02670(TDEL0C02670)
KAF: 
KAFR_0L01850(KAFR0L01850)
PPA: 
DHA: 
PIC: 
PGU: 
SPAA: 
LEL: 
CAL: 
CTP: 
COT: 
CDU: 
CTEN: 
YLI: 
CLU: 
NCR: 
SMP: 
PAN: 
TTT: 
MTM: 
CTHR: 
MGR: 
TMN: 
FGR: 
NHE: 
TRE: 
MAW: 
MAJ: 
CMT: 
VAL: 
ELA: 
SSL: 
BFU: 
MBE: 
ANI: 
AFM: 
AOR: 
AOR_1_556094(AO090120000319)
AFV: 
ACT: 
NFI: 
PCS: 
CIM: 
CPW: 
PBL: 
URE: 
ABE: 
TVE: 
AJE: 
PNO: 
PTE: 
BZE: 
BSC: 
BOR: 
ZTR: 
PFJ: 
BCOM: 
NPA: 
TML: 
SPO: 
CNE: 
CNB: 
CGI: 
TMS: 
DSQ: 
SHS: 
PCO: 
PSQ: 
ADL: 
FME: 
GTR: 
LBC: 
MPR: 
MRR: 
CCI: 
SCM: 
ABP: 
AGABI1DRAFT118456(AGABI1DRAFT_118456)
ABV: 
AGABI2DRAFT200258(AGABI2DRAFT_200258)
CPUT: 
SLA: 
PGR: 
MLR: 
WSE: 
MBR: 
DDI: 
DPP: 
DFA: 
ACAN: 
PTI: 
TPS: 
PIF: 
EHX: 
GTT: 
TBR: 
TCR: 
LMA: 
LIF: 
LDO: 
LMI: 
LBZ: 
NGR: 
 » show all
Taxonomy
Reference
1  [PMID:11308030]
  Authors
Sharma CB, Knauer R, Lehle L
  Title
Biosynthesis of lipid-linked oligosaccharides in yeast: the ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.
  Journal
Biol. Chem. 382 (2001) 321-8.
  Sequence
[sce:YBL082C]
Reference
2  [PMID:10660594]
  Authors
Cipollo JF, Trimble RB
  Title
The accumulation of Man(6)GlcNAc(2)-PP-dolichol in the Saccharomyces cerevisiae Deltaalg9 mutant reveals a regulatory role for the Alg3p alpha1,3-Man middle-arm  addition in downstream oligosaccharide-lipid and glycoprotein glycan processing.
  Journal
J. Biol. Chem. 275 (2000) 4267-77.
Other DBs
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IUBMB Enzyme Nomenclature: 
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