KEGG   ENZYME: 2.4.1.265Help
Entry
EC 2.4.1.265                Enzyme                                 

Name
dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase;
ALG8;
Dol-P-Glc:Glc1Man9GlcNAc2-PP-Dol alpha-1,3-glucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
dolichyl beta-D-glucosyl phosphate:D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,3-glucosyltransferase
Reaction(IUBMB)
dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate [RN:R06263]
Reaction(KEGG)
R06263(G)
Show
Substrate
dolichyl beta-D-glucosyl phosphate [CPD:C01246];
D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
Product
D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol;
dolichyl phosphate [CPD:C00110]
Comment
The successive addition of three glucose residues by EC 2.4.1.267 (dolichyl-P-Glc:Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase), EC 2.4.1.265 and EC 2.4.1.256 (dolichyl-P-Glc:Glc2Man9GlcNAc2-PP-dolichol alpha-1,2-glucosyltransferase) represents the final stage of the lipid-linked oligosaccharide assembly.
History
EC 2.4.1.265 created 2011, modified 2012
Pathway
N-Glycan biosynthesis
Metabolic pathways
Orthology
K03849  
alpha-1,3-glucosyltransferase
Genes
HSA: 
79053(ALG8)
PTR: 
451446(ALG8)
PPS: 
100976025(ALG8)
GGO: 
101143793(ALG8)
PON: 
100172957(ALG8)
MCC: 
699834(ALG8)
MCF: 
102118129(ALG8)
MMU: 
381903(Alg8)
RNO: 
293129(Alg8)
CGE: 
100766150(Alg8)
HGL: 
101717763(Alg8)
TUP: 
102489591(ALG8)
CFA: 
476793(ALG8)
AML: 
FCA: 
101080479(ALG8)
BTA: 
538731(ALG8)
BOM: 
102278012(ALG8)
PHD: 
102325804(ALG8)
CHX: 
102177868(ALG8)
SSC: 
100511030(ALG8)
CFR: 
102517495(ALG8)
ECB: 
100051152(ALG8)
MYB: 
102252684(ALG8)
MDO: 
100027315(ALG8)
SHR: 
100925953(ALG8)
GGA: 
428097(ALG8)
MGP: 
100542312(ALG8)
TGU: 
100232627(ALG8)
FAB: 
101817964(ALG8)
PHI: 
102099307(ALG8)
APLA: 
101792588(ALG8)
FPG: 
101920638(ALG8)
FCH: 
102056588(ALG8)
CLV: 
102090337(ALG8)
ASN: 
102377483(ALG8)
PSS: 
102458309(ALG8)
ACS: 
100567213(alg8)
XLA: 
431849(alg8)
XTR: 
548388(alg8)
DRE: 
327601(alg8)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
102363012(ALG8)
BFO: 
CIN: 
SPU: 
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
AGA: 
AAG: 
CQU: 
AME: 
551841(GB14100)
NVI: 
TCA: 
BMOR: 
PHU: 
ISC: 
CBR: 
BMY: 
LOA: 
TSP: 
SMM: 
NVE: 
HMG: 
TAD: 
AQU: 
ATH: 
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
GMX: 
MTR: 
CAM: 
FVE: 
CSV: 
RCU: 
POP: 
VVI: 
SLY: 
SOT: 
OSA: 
DOSA: 
Os02t0688500-01(Os02g0688500)
BDI: 
SBI: 
SORBI_04g031440(SORBIDRAFT_04g031440)
SITA: 
SMO: 
PPP: 
CRE: 
VCN: 
MIS: 
MPP: 
CSL: 
CVR: 
CME: 
GSL: 
CCP: 
SCE: 
YOR067C(ALG8)
AGO: 
ERC: 
KLA: 
LTH: 
PPA: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0G00450(NCAS0G00450)
NDI: 
NDAI_0F00510(NDAI0F00510)
TPF: 
TPHA_0J01380(TPHA0J01380)
TBL: 
TBLA_0A09310(TBLA0A09310)
TDL: 
TDEL_0B02540(TDEL0B02540)
KAF: 
KAFR_0D02440(KAFR0D02440)
DHA: 
PIC: 
PGU: 
LEL: 
CAL: 
CTP: 
CDU: 
COT: 
YLI: 
CLU: 
NCR: 
SMP: 
PAN: 
TTT: 
MTM: 
MGR: 
FGR: 
NHE: 
VAL: 
SSL: 
BFU: 
ANI: 
NFI: 
AFM: 
AOR: 
AOR_1_252134(AO090102000146)
AFV: 
ACT: 
PCS: 
CIM: 
CPW: 
PBL: 
URE: 
ABE: 
TVE: 
AJE: 
PNO: 
ZTR: 
TML: 
SPO: 
LBC: 
CCI: 
SCM: 
UMA: 
MGL: 
PGR: 
DDI: 
DPP: 
ACAN: 
TGO: 
PTM: 
PTI: 
TPS: 
PIF: 
EHX: 
GTT: 
NGR: 
 » show all
Taxonomy
Reference
1  [PMID:8016100]
  Authors
Stagljar I, te Heesen S, Aebi M
  Title
New phenotype of mutations deficient in glucosylation of the lipid-linked oligosaccharide: cloning of the ALG8 locus.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 91 (1994) 5977-81.
  Organism
Saccharomyces cerevisiae [GN:sce]
  Sequence
[sce:YOR067C]
Reference
2  [PMID:3536907]
  Authors
Runge KW, Robbins PW
  Title
A new yeast mutation in the glucosylation steps of the asparagine-linked glycosylation pathway. Formation of a novel asparagine-linked oligosaccharide containing two glucose residues.
  Journal
J. Biol. Chem. 261 (1986) 15582-90.
  Organism
Saccharomyces cerevisiae [GN:sce]
  Sequence
[sce:YOR067C]
Reference
3  [PMID:12480927]
  Authors
Chantret I, Dancourt J, Dupre T, Delenda C, Bucher S, Vuillaumier-Barrot S, Ogier de Baulny H, Peletan C, Danos O, Seta N, Durand G, Oriol R, Codogno P, Moore SE
  Title
A deficiency in dolichyl-P-glucose:Glc1Man9GlcNAc2-PP-dolichyl alpha3-glucosyltransferase defines a new subtype of congenital disorders of glycosylation.
  Journal
J. Biol. Chem. 278 (2003) 9962-71.
  Organism
Homo sapiens [GN:hsa]
  Sequence
[hsa:79053]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

  All links  
Ontology (4)   
   KEGG BRITE (4)   
Pathway (111)   
   KEGG PATHWAY (5)   
   KEGG MODULE (106)   
Chemical substance (6)   
   KEGG COMPOUND (2)   
   KEGG GLYCAN (4)   
Chemical reaction (3)   
   KEGG ENZYME (2)   
   KEGG REACTION (1)   
Genome (2)   
   KEGG GENOME (2)   
Gene (263)   
   KEGG ORTHOLOGY (1)   
   KEGG GENES (183)   
   KEGG DGENES (21)   
   KEGG MGENES (58)   
Protein sequence (50)   
   UniProt (21)   
   SWISS-PROT (20)   
   RefSeq(pep) (9)   
DNA sequence (13)   
   RefSeq(nuc) (11)   
   GenBank (1)   
   EMBL (1)   
Literature (3)   
   PubMed (3)   
Enzyme (4)   
   BRENDA (1)   
   EXPASY-ENZYME (1)   
   EXPLORENZ (1)   
   IUBMB (1)   
Others (108)   
   T20001 (1)   
   T20002 (20)   
   T20003 (3)   
   T20004 (2)   
   T20005 (7)   
   T20006 (16)   
   T20007 (1)   
   T20008 (2)   
   T20009 (3)   
   T20010 (1)   
   T20011 (1)   
   T20013 (1)   
   T20014 (2)   
   T20016 (2)   
   T20020 (1)   
   T20021 (1)   
   T20023 (1)   
   T20025 (1)   
   T20026 (2)   
   T20028 (1)   
   T20029 (2)   
   T20032 (1)   
   T20033 (1)   
   T20042 (1)   
   T20043 (1)   
   T20045 (1)   
   T20050 (1)   
   T20054 (1)   
   T20056 (1)   
   T20057 (1)   
   T20059 (1)   
   T20060 (3)   
   T20066 (1)   
   T20069 (1)   
   T20082 (1)   
   T20083 (2)   
   T20086 (2)   
   T20088 (1)   
   T20092 (4)   
   T20095 (1)   
   T20096 (1)   
   T20097 (4)   
   T20099 (1)   
   T20101 (3)   
   T20105 (2)   
All databases (567)   

Download RDF
DBGET integrated database retrieval system