KEGG   ENZYME: 2.4.1.265Help
Entry
EC 2.4.1.265                Enzyme                                 

Name
dolichyl-P-Glc:Glc1Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase;
ALG8;
Dol-P-Glc:Glc1Man9GlcNAc2-PP-Dol alpha-1,3-glucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
dolichyl beta-D-glucosyl phosphate:D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol alpha-1,3-glucosyltransferase
Reaction(IUBMB)
dolichyl beta-D-glucosyl phosphate + D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate [RN:R06263]
Reaction(KEGG)
R06263(G)
Show
Substrate
dolichyl beta-D-glucosyl phosphate [CPD:C01246];
D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol
Product
D-Glc-alpha-(1->3)-D-Glc-alpha-(1->3)-D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->6)]-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol;
dolichyl phosphate [CPD:C00110]
Comment
The successive addition of three glucose residues by EC 2.4.1.267 (dolichyl-P-Glc:Man9GlcNAc2-PP-dolichol alpha-1,3-glucosyltransferase), EC 2.4.1.265 and EC 2.4.1.256 (dolichyl-P-Glc:Glc2Man9GlcNAc2-PP-dolichol alpha-1,2-glucosyltransferase) represents the final stage of the lipid-linked oligosaccharide assembly.
History
EC 2.4.1.265 created 2011, modified 2012
Pathway
N-Glycan biosynthesis
Metabolic pathways
Orthology
K03849  
alpha-1,3-glucosyltransferase
Genes
HSA: 
79053(ALG8)
PTR: 
451446(ALG8)
PPS: 
100976025(ALG8)
GGO: 
101143793(ALG8)
PON: 
100172957(ALG8)
NLE: 
100583648(ALG8)
MCC: 
699834(ALG8)
MCF: 
102118129(ALG8)
CJC: 
100410610(ALG8)
MMU: 
381903(Alg8)
RNO: 
293129(Alg8)
CGE: 
100766150(Alg8)
NGI: 
103742996(Alg8)
HGL: 
101717763(Alg8)
OCU: 
100350058(ALG8)
TUP: 
102489591(ALG8)
CFA: 
476793(ALG8)
AML: 
UMR: 
103678139(ALG8)
FCA: 
101080479(ALG8)
PTG: 
102966535(ALG8)
BTA: 
538731(ALG8)
BOM: 
102278012(ALG8)
PHD: 
102325804(ALG8)
CHX: 
102177868(ALG8)
OAS: 
101109656(ALG8)
SSC: 
100511030(ALG8)
CFR: 
102517495(ALG8)
BACU: 
103014128(ALG8)
LVE: 
103079942(ALG8)
ECB: 
100051152(ALG8)
MYB: 
102252684(ALG8)
MYD: 
102758490(ALG8)
PALE: 
102886309(ALG8)
MDO: 
100027315(ALG8)
SHR: 
100925953(ALG8)
OAA: 
100090090(ALG8)
GGA: 
428097(ALG8)
MGP: 
100542312(ALG8)
APLA: 
101792588(ALG8)
TGU: 
100232627(ALG8)
FAB: 
101817964(ALG8)
PHI: 
102099307(ALG8)
FPG: 
101920638(ALG8)
FCH: 
102056588(ALG8)
CLV: 
102090337(ALG8)
ASN: 
102377483(ALG8)
AMJ: 
102569717(ALG8)
PSS: 
102458309(ALG8)
CMY: 
102937209(ALG8)
ACS: 
100567213(alg8)
PBI: 
103055334(ALG8)
XLA: 
431849(alg8)
XTR: 
548388(alg8)
DRE: 
327601(alg8)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
102363012(ALG8)
CMK: 
103177229(alg8)
BFO: 
CIN: 
SPU: 
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
MDE: 
AGA: 
AAG: 
CQU: 
AME: 
551841(GB14100)
NVI: 
TCA: 
BMOR: 
API: 
PHU: 
ISC: 
CBR: 
BMY: 
LOA: 
TSP: 
HRO: 
LGI: 
SMM: 
NVE: 
HMG: 
TAD: 
AQU: 
ATH: 
ALY: 
CRB: 
EUS: 
BRP: 
CIT: 
CIC: 
TCC: 
GMX: 
PVU: 
MTR: 
CAM: 
FVE: 
PPER: 
PMUM: 
MDM: 
PXB: 
CSV: 
CMO: 
RCU: 
POP: 
VVI: 
SLY: 
SOT: 
OSA: 
DOSA: 
Os02t0688500-01(Os02g0688500)
OBR: 
BDI: 
SBI: 
SORBI_04g031440(SORBIDRAFT_04g031440)
ZMA: 
SITA: 
PDA: 
MUS: 
ATR: 
s00092p00046430(AMTR_s00092p00046430)
SMO: 
PPP: 
CRE: 
VCN: 
MIS: 
MPP: 
CSL: 
CVR: 
CME: 
GSL: 
CCP: 
SCE: 
YOR067C(ALG8)
AGO: 
ERC: 
KLA: 
LTH: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0G00450(NCAS0G00450)
NDI: 
NDAI_0F00510(NDAI0F00510)
TPF: 
TPHA_0J01380(TPHA0J01380)
TBL: 
TBLA_0A09310(TBLA0A09310)
TDL: 
TDEL_0B02540(TDEL0B02540)
KAF: 
KAFR_0D02440(KAFR0D02440)
PPA: 
DHA: 
PIC: 
PGU: 
SPAA: 
LEL: 
CAL: 
CTP: 
COT: 
CDU: 
CTEN: 
YLI: 
CLU: 
NCR: 
SMP: 
PAN: 
TTT: 
MTM: 
CTHR: 
MGR: 
TMN: 
FGR: 
NHE: 
TRE: 
MAW: 
MAJ: 
CMT: 
VAL: 
ELA: 
SSL: 
BFU: 
MBE: 
ANI: 
AFM: 
AOR: 
AOR_1_252134(AO090102000146)
AFV: 
ACT: 
NFI: 
PCS: 
CIM: 
CPW: 
PBL: 
URE: 
ABE: 
TVE: 
AJE: 
PNO: 
PTE: 
BZE: 
BSC: 
BOR: 
ZTR: 
PFJ: 
BCOM: 
NPA: 
TML: 
SPO: 
DSQ: 
PCO: 
SHS: 
PSQ: 
ADL: 
FME: 
GTR: 
LBC: 
MRR: 
CCI: 
SCM: 
ABP: 
AGABI1DRAFT73545(AGABI1DRAFT_73545)
ABV: 
AGABI2DRAFT202561(AGABI2DRAFT_202561)
CPUT: 
UMA: 
PFP: 
MGL: 
PGR: 
MLR: 
DDI: 
DPP: 
ACAN: 
TGO: 
PTM: 
PTI: 
TPS: 
PIF: 
EHX: 
GTT: 
NGR: 
 » show all
Taxonomy
Reference
1  [PMID:8016100]
  Authors
Stagljar I, te Heesen S, Aebi M
  Title
New phenotype of mutations deficient in glucosylation of the lipid-linked oligosaccharide: cloning of the ALG8 locus.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 91 (1994) 5977-81.
  Sequence
[sce:YOR067C]
Reference
2  [PMID:3536907]
  Authors
Runge KW, Robbins PW
  Title
A new yeast mutation in the glucosylation steps of the asparagine-linked glycosylation pathway. Formation of a novel asparagine-linked oligosaccharide containing two glucose residues.
  Journal
J. Biol. Chem. 261 (1986) 15582-90.
  Sequence
[sce:YOR067C]
Reference
3  [PMID:12480927]
  Authors
Chantret I, Dancourt J, Dupre T, Delenda C, Bucher S, Vuillaumier-Barrot S, Ogier de Baulny H, Peletan C, Danos O, Seta N, Durand G, Oriol R, Codogno P, Moore SE
  Title
A deficiency in dolichyl-P-glucose:Glc1Man9GlcNAc2-PP-dolichyl alpha3-glucosyltransferase defines a new subtype of congenital disorders of glycosylation.
  Journal
J. Biol. Chem. 278 (2003) 9962-71.
  Sequence
[hsa:79053]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system