KEGG   ENZYME: 2.4.1.65Help
Entry
EC 2.4.1.65                 Enzyme                                 

Name
3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase;
(Lea)-dependent (alpha-3/4)-fucosyltransferase;
alpha(1,3/1,4) fucosyltransferase III;
alpha-(1->4)-L-fucosyltransferase;
alpha-4-L-fucosyltransferase;
beta-acetylglucosaminylsaccharide fucosyltransferase;
FucT-II;
Lewis alpha-(1->3/4)-fucosyltransferase;
Lewis blood group alpha-(1->3/4)-fucosyltransferase;
Lewis(Le) blood group gene-dependent alpha-(1->3/4)-L-fucosyltransferase;
blood group Lewis alpha-4-fucosyltransferase;
blood-group substance Lea-dependent fucosyltransferase;
guanosine diphosphofucose-beta-acetylglucosaminylsaccharide 4-alpha-L-fucosyltransferase;
guanosine diphosphofucose-glycoprotein 4-alpha-L-fucosyltransferase;
guanosine diphosphofucose-glycoprotein 4-alpha-fucosyltransferase;
3-alpha-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase;
GDP-beta-L-fucose:3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R 4I-alpha-L-fucosyltransferase;
GDP-L-fucose:3-beta-D-galactosyl-N-acetyl-D-glucosaminyl-R 4I-alpha-L-fucosyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
GDP-beta-L-fucose:beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R 4I-alpha-L-fucosyltransferase
Reaction(IUBMB)
GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R = GDP + beta-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->4)]-N-acetyl-beta-D-glucosaminyl-R [RN:R04553]
Reaction(KEGG)
R04553 > R06155(G);
(other) R06162(G) R06163(G) R06164(G) R06165(G)
Show
Substrate
GDP-beta-L-fucose [CPD:C00325];
beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-R
Product
GDP [CPD:C00035];
beta-D-galactosyl-(1->3)-[alpha-L-fucosyl-(1->4)]-N-acetyl-beta-D-glucosaminyl-R
Comment
This enzyme is the product of the Lewis blood group gene. Normally acts on a glycoconjugate where R (see reaction) is a glycoprotein or glycolipid. Although it is a 4-fucosyltransferase, it has a persistent 3-fucosyltransferase activity towards the glucose residue in free lactose. This enzyme fucosylates on O-4 of an N-acetylglucosamine that carries a galactosyl group on O-3, unlike EC 2.4.1.152, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, which fucosylates on O-3 of an N-acetylglucosamine that carries a galactosyl group on O-4. Enzymes catalysing the 4-alpha-fucosylation of the GlcNAc in beta-D-Gal-(1->3)-beta-GlcNAc sequences (with some activity also as 3-alpha-fucosyltransferases) are present in plants, where the function in vivo is the modification of N-glycans. In addition, the fucTa gene of Helicobacter strain UA948 encodes a fucosyltransferase with both 3-alpha- and 4-alpha-fucosyltransferase activities.
History
EC 2.4.1.65 created 1972, modified 2001, modified twice 2002
Pathway
Glycosphingolipid biosynthesis - lacto and neolacto series
Metabolic pathways
Orthology
K00716  
galactoside 3(4)-fucosyltransferase 3
K07633  
galactoside alpha-1,3-fucosyltransferase 5
K07634  
galactoside alpha-1,3-fucosyltransferase 6
Genes
HSA: 
2525(FUT3) 2527(FUT5) 2528(FUT6)
PTR: 
455628(FUT5) 493975(FUT3) 493976(FUT6)
PPS: 
100980873(FUT6) 100982106(FUT5) 100982431(FUT3)
GGO: 
PON: 
MCC: 
697582(FUT3)
MCF: 
NGI: 
HGL: 
CFA: 
449024(FUT5)
AML: 
FCA: 
101093666(FUT5)
PTG: 
102972414(FUT3)
BTA: 
338077(FUT5)
BOM: 
102271549(FUT3)
PHD: 
102335338(FUT3)
CHX: 
102181295(FUT3)
OAS: 
101108745(FUT3)
SSC: 
100513844(FUT3)
CFR: 
102504974(FUT3)
BACU: 
103005588(FUT3)
LVE: 
103076464(FUT3)
MYB: 
102255875(FUT3)
MDO: 
100015438(FUT3)
OAA: 
GGA: 
428341(FUT5)
APLA: 
FPG: 
101918531(FUT3)
FCH: 
102058652(FUT3)
PSS: 
CMY: 
102942494(FUT3)
PBI: 
XLA: 
399049(fut6)
XTR: 
 » show all
Taxonomy
Reference
1  [PMID:7287719]
  Authors
Prieels JP, Monnom D, Dolmans M, Beyer TA, Hill RL.
  Title
Co-purification of the Lewis blood group N-acetylglucosaminide alpha 1 goes to 4 fucosyltransferase and an N-acetylglucosaminide alpha 1 goes to 3 fucosyltransferase from human milk.
  Journal
J. Biol. Chem. 256 (1981) 10456-63.
Reference
2  [PMID:10671538]
  Authors
Rasko DA, Wang G, Palcic MM, Taylor DE.
  Title
Cloning and characterization of the alpha(1,3/4) fucosyltransferase of Helicobacter pylori.
  Journal
J. Biol. Chem. 275 (2000) 4988-94.
Reference
3  [PMID:12084979]
  Authors
Wilson IB.
  Title
Identification of a cDNA encoding a plant Lewis-type alpha1,4-fucosyltransferase.
  Journal
Glycoconj. J. 18 (2001) 439-47.
  Sequence
[sly:543777]
Reference
4  [PMID:12676935]
  Authors
Ma B, Wang G, Palcic MM, Hazes B, Taylor DE.
  Title
C-terminal amino acids of Helicobacter pylori alpha1,3/4 fucosyltransferases determine type I and type II transfer.
  Journal
J. Biol. Chem. 278 (2003) 21893-900.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
37277-69-3

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