KEGG   ENZYME: 2.4.1.79Help
Entry
EC 2.4.1.79                 Enzyme                                 
Name
globotriaosylceramide 3-beta-N-acetylgalactosaminyltransferase;
uridine diphosphoacetylgalactosamine-galactosylgalactosylglucosylceramide acetylgalactosaminyltransferase;
globoside synthetase;
UDP-N-acetylgalactosamine:globotriaosylceramide beta-3-N-acetylgalactosaminyltransferase;
galactosylgalactosylglucosylceramide beta-D-acetylgalactosaminyltransferase;
UDP-N-acetylgalactosamine:globotriaosylceramide beta1,3-N-acetylgalactosaminyltransferase;
globoside synthase;
gUDP-N-acetyl-D-galactosamine:D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide beta-N-acetyl-D-galactosaminyltransferase;
beta3GalNAc-T1;
UDP-N-acetyl-D-galactosamine:alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosylceramide 3III-beta-N-acetyl-D-galactosaminyltransferase;
UDP-N-acetyl-D-galactosamine:alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide 3III-beta-N-acetyl-D-galactosaminyltransferase;
UDP-N-acetyl-D-galactosamine:alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide III3-beta-N-acetyl-D-galactosaminyltransferase
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
BRITE hierarchy
Sysname
UDP-N-acetyl-alpha-D-galactosamine:alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide III3-beta-N-acetyl-D-galactosaminyltransferase
Reaction(IUBMB)
UDP-N-acetyl-alpha-D-galactosamine + alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + N-acetyl-beta-D-galactosaminyl-(1->3)-alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide [RN:R05631 R05962]
Reaction(KEGG)
Substrate
UDP-N-acetyl-alpha-D-galactosamine [CPD:C00203];
alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide [CPD:C04737]
Product
UDP [CPD:C00015];
N-acetyl-beta-D-galactosaminyl-(1->3)-alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
Comment
Globoside is a neutral glycosphingolipid in human erythrocytes and has blood-group-P-antigen activity [4]. The enzyme requires a divalent cation for activity, with Mn2+ required for maximal activity [3]. UDP-GalNAc is the only sugar donor that is used efficiently by the enzyme: UDP-Gal and UDP-GlcNAc result in very low enzyme activity [3]. Lactosylceramide, globoside and gangliosides GM3 and GD3 are not substrates [4]. For explanation of the superscripted '3' in the systematic name, see {iupac/misc/glylp#5.3::GL-5.3.4}.
Pathway
Glycosphingolipid biosynthesis - globo series
Metabolic pathways
Orthology
K00719  
galactosylgalactosylglucosylceramide beta-D-acetylgalactosaminyltransferase
Genes
HSA: 
8706(B3GALNT1)
PTR: 
460824(B3GALNT1)
GGO: 
101128316(B3GALNT1)
PON: 
100174453(B3GALNT1)
MCC: 
703939(B3GALNT1)
MMU: 
26879(B3galnt1)
RNO: 
310508(B3galnt1)
CFA: 
100683634(B3GALNT1)
AML: 
FCA: 
101092536(B3GALNT1)
BTA: 
767844(B3GALNT1)
SSC: 
397634(B3GALNT1)
ECB: 
100062101
MDO: 
100015392
SHR: 
100914641(B3GALNT1)
OAA: 
100077633
GGA: 
425010(B3GALNT1)
MGP: 
100542332
TGU: 
100228838(B3GALNT1)
ACS: 
100564179
 » show all
Taxonomy
Reference
1  [PMID:4632917]
  Authors
Chien JL, Williams T, Basu S.
  Title
Biosynthesis of a globoside-type glycosphingolipid by a  -N-acetylgalactosaminyltransferase from embryonic chicken brain.
  Journal
J. Biol. Chem. 248 (1973) 1778-85.
  Organism
Gallus gallus [GN:gga]
Reference
2  [PMID:4433547]
  Authors
Ishibashi T, Kijimoto S, Makita A.
  Title
Biosynthesis of globoside and Forssman hapten from trihexosylceramide and properties of beta-N-acetyl-galactosaminyltransferase of guinea pig kidney.
  Journal
Biochim. Biophys. Acta. 337 (1974) 92-106.
  Organism
Cavia porcellus
Reference
3  [PMID:6425294]
  Authors
Taniguchi N, Makita A.
  Title
Purification and characterization of UDP-N-acetylgalactosamine: globotriaosylceramide beta-3-N-acetylgalactosaminyltransferase, a synthase of human blood group P antigen, from canine spleen.
  Journal
J. Biol. Chem. 259 (1984) 5637-42.
  Organism
Canis familiaris [GN:cfa]
Reference
4  [PMID:10993897]
  Authors
Okajima T, Nakamura Y, Uchikawa M, Haslam DB, Numata SI, Furukawa K, Urano T, Furukawa K.
  Title
Expression cloning of human globoside synthase cDNAs. Identification of beta 3Gal-T3 as UDP-N-acetylgalactosamine:globotriaosylceramide beta 1,3-N-acetylgalactosaminyltransferase.
  Journal
J. Biol. Chem. 275 (2000) 40498-503.
  Organism
Homo sapiens [GN:hsa]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
62213-46-1
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