| Entry |
|
|---|
| Name |
cyanohydrin beta-glucosyltransferase;
uridine diphosphoglucose-p-hydroxymandelonitrile
glucosyltransferase;
UDP-glucose-p-hydroxymandelonitrile glucosyltransferase;
uridine diphosphoglucose-cyanohydrin glucosyltransferase;
uridine diphosphoglucose:aldehyde cyanohydrin
beta-glucosyltransferase;
UDP-glucose:(S)-4-hydroxymandelonitrile beta-D-glucosyltransferase;
UGT85B1;
UDP-glucose:p-hydroxymandelonitrile-O-glucosyltransferase |
|---|
| Class |
Transferases;
Glycosyltransferases;
Hexosyltransferases
 |
|---|
| Sysname |
UDP-D-glucose:(S)-4-hydroxymandelonitrile beta-D-glucosyltransferase |
|---|
| Reaction(IUBMB) |
UDP-D-glucose + (S)-4-hydroxymandelonitrile = UDP +
(S)-4-hydroxymandelonitrile beta-D-glucoside [RN:R04296] |
|---|
| Reaction(KEGG) |
R04296;
(other) R04948
 |
|---|
| Substrate |
UDP-D-glucose [CPD:C00029];
(S)-4-hydroxymandelonitrile [CPD:C03742] |
|---|
| Product |
UDP [CPD:C00015];
(S)-4-hydroxymandelonitrile beta-D-glucoside [CPD:C05143] |
|---|
| Comment |
Acts on a wide range of substrates in vitro, including cyanohydrins,
terpenoids, phenolics, hexanol derivatives and plant hormones, in a
regiospecific manner [3]. This enzyme is involved in the
biosynthesis of the cyanogenic glucoside dhurrin in sorghum, along
with EC 1.14.13.41, tyrosine N-monooxygenase and EC 1.14.13.68,
4-hydroxyphenylacetaldehyde oxime monooxygenase. This reaction
prevents the disocciation and release of toxic hydrogen cyanide [3]. |
|---|
| Pathway |
PATH: ec00460 Cyanoamino acid metabolism |
|---|
| Orthology |
KO: K13030 cyanohydrin beta-glucosyltransferase |
|---|
| Genes |
SBI: SORBI_01g001220(SORBIDRAFT_01g001220)
 |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:4416442]
Reay PF, Conn EE.
The purification and properties of a uridine diphosphate glucose:
aldehyde cyanohydrin beta-glucosyltransferase from sorghum
seedlings.
J. Biol. Chem. 249 (1974) 5826-30.
Sorghum bicolor [GN:sbi] |
|---|
Reference
Authors
Title
Journal
Organism
|
2 [PMID:10585420]
Jones PR, Moller BL, Hoj PB.
The UDP-glucose:p-hydroxymandelonitrile-O-glucosyltransferase that
catalyzes the last step in synthesis of the cyanogenic glucoside
dhurrin in Sorghum bicolor. Isolation, cloning, heterologous
expression, and substrate specificity.
J. Biol. Chem. 274 (1999) 35483-91.
Sorghum bicolor [GN:sbi] |
|---|
Reference
Authors
Title
Journal
Organism
|
3 [PMID:12946413]
Hansen KS, Kristensen C, Tattersall DB, Jones PR, Olsen CE, Bak S,
Moller BL.
The in vitro substrate regiospecificity of recombinant UGT85B1, the
cyanohydrin glucosyltransferase from Sorghum bicolor.
Phytochemistry. 64 (2003) 143-51.
Sorghum bicolor [GN:sbi] |
|---|
Reference
Authors
Title
Journal
Organism
|
4 [PMID:12114576]
Busk PK, Moller BL.
Dhurrin synthesis in sorghum is regulated at the transcriptional
level and induced by nitrogen fertilization in older plants.
Plant. Physiol. 129 (2002) 1222-31.
Sorghum bicolor [GN:sbi] |
|---|
Reference
Authors
Title
Journal
Organism
|
5 [PMID:15665094]
Kristensen C, Morant M, Olsen CE, Ekstrom CT, Galbraith DW, Moller
BL, Bak S.
Metabolic engineering of dhurrin in transgenic Arabidopsis plants
with marginal inadvertent effects on the metabolome and
transcriptome.
Proc. Natl. Acad. Sci. U. S. A. 102 (2005) 1779-84.
Sorghum bicolor [GN:sbi] |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 2.4.1.85
IUBMB Enzyme Nomenclature: 2.4.1.85
ExPASy - ENZYME nomenclature database: 2.4.1.85
BRENDA, the Enzyme Database: 2.4.1.85
CAS: 55354-52-4 |
|---|
