KEGG   ENZYME: 2.4.2.43Help
Entry
EC 2.4.2.43                 Enzyme                                 

Name
lipid IVA 4-amino-4-deoxy-L-arabinosyltransferase;
undecaprenyl phosphate-alpha-L-Ara4N transferase;
4-amino-4-deoxy-L-arabinose lipid A transferase;
polymyxin resistance protein PmrK;
arnT (gene name)
Class
Transferases;
Glycosyltransferases;
Pentosyltransferases
BRITE hierarchy
Sysname
4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate:lipid IVA 4-amino-4-deoxy-L-arabinopyranosyltransferase
Reaction(IUBMB)
(1) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A = alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A + ditrans,octacis-undecaprenyl phosphate [RN:R09781];
(2) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + lipid IVA = lipid IIA + ditrans,octacis-undecaprenyl phosphate [RN:R09773];
(3) 4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate + alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA = 4'-alpha-L-Ara4N-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA + ditrans,octacis-undecaprenyl phosphate [RN:R09774]
Reaction(KEGG)
Substrate
4-amino-4-deoxy-alpha-L-arabinopyranosyl ditrans,octacis-undecaprenyl phosphate [CPD:C16157];
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid A [CPD:C06026];
lipid IVA;
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA
Product
alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-[4-P-L-Ara4N]-lipid A [CPD:C19890];
ditrans,octacis-undecaprenyl phosphate [CPD:C17556];
lipid IIA [CPD:C19884];
4'-alpha-L-Ara4N-alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA
Comment
Integral membrane protein present in the inner membrane of certain Gram negative endobacteria. In strains that do not produce 3-deoxy-D-manno-octulosonic acid (Kdo), the enzyme adds a single arabinose unit to the 1-phosphate moiety of the tetra-acylated lipid A precursor, lipid IVA. In the presence of a Kdo disaccharide, the enzyme primarily adds an arabinose unit to the 4-phosphate of lipid A molecules. The Salmonella typhimurium enzyme can add arabinose units to both positions.
History
EC 2.4.2.43 created 2010, modified 2011
Orthology
K07264  
4-amino-4-deoxy-L-arabinose transferase
Genes
ECO: 
b2257(arnT)
ECJ: 
Y75_p2220(arnT)
ECD: 
EBW: 
BWG_2030(arnT)
ECOK: 
ECE: 
Z3515(arnT)
ECS: 
ECs3145(arnT)
ECF: 
ETW: 
ECSP_3134(arnT)
ELX: 
EOJ: 
EOI: 
EOH: 
ECG: 
EOK: 
ELR: 
ECC: 
c2799(arnT)
ECP: 
ECP_2300(arnT)
ECI: 
ECV: 
ECX: 
ECW: 
ECM: 
ECY: 
ECSE_2516(arnT)
ECR: 
ECQ: 
ECK: 
ECT: 
EOC: 
CE10_2640(arnT)
EUM: 
ECZ: 
ELO: 
ELN: 
ELH: 
ESE: 
ESO: 
O3O_17430(arnT)
ESM: 
O3M_08155(arnT)
ESL: 
O3K_08205(arnT)
ECL: 
EBR: 
ECB_02183(arnT)
EBD: 
ECBD_1402(arnT)
EKO: 
EKF: 
EAB: 
EDH: 
EDJ: 
EIH: 
ENA: 
ELU: 
EUN: 
ELW: 
ECW_m2448(arnT)
ELL: 
WFL_11980(arnT)
ELC: 
i14_2598(arnT)
ELD: 
i02_2598(arnT)
ELP: 
EBL: 
ECD_02183(arnT)
EBE: 
B21_02142(arnT)
ELF: 
LF82_0139(arnT)
ECOA: 
ECOL: 
ECOI: 
ECOJ: 
ECOO: 
EFE: 
EFER_0912(arnT)
STY: 
STT: 
t0562(arnT)
SEX: 
SENT: 
STM: 
STM2301(arnT)
SEO: 
SEV: 
SEY: 
SEM: 
SEJ: 
SEB: 
SEF: 
SETU: 
SETC: 
SEEN: 
SENR: 
SEND: 
SPT: 
SPA0562(pqaB)
SEK: 
SSPA0526(arnT)
SPQ: 
SEI: 
SPC_1410(arnT)
SEC: 
SC2302(arnT)
SEH: 
SENH: 
SEEH: 
SEE: 
SENN: 
SEW: 
SEA: 
SENS: 
SED: 
SeD_A2645(arnT)
SEG: 
SG2330(arnT)
SEL: 
SPUL_0589(pqaB)
SEGA: 
SET: 
SEN2283(arnT)
SENJ: 
SEEC: 
SEEB: 
SEEP: 
SENB: 
BN855_23820(SBOV23611)
SENE: 
IA1_11460(arnT)
SES: 
SBG: 
SBG_2090(pqaB)
SBZ: 
YPE: 
YPO2418(arnT)
YPK: 
y1921(arnT)
YPA: 
YPA_1762(arnT)
YPN: 
YPN_1872(arnT)
YPM: 
YP_2205(arnT)
YPP: 
YPG: 
YPZ: 
YPT: 
YPD: 
YPX: 
YPH: 
YPC_2053(arnT)
YPS: 
YPTB2326(arnT)
YPI: 
YPY: 
YPK_1835(arnT)
YPB: 
YPTS_2401(arnT)
YEN: 
YE2188(arnT)
YEP: 
YEY: 
YSI: 
SFL: 
SF2336(arnT)
SFX: 
S2469(arnT)
SFV: 
SFV_2327(arnT)
SFE: 
SFxv_2579(arnT)
SSN: 
SSON_2318(arnT)
SSJ: 
SBO: 
SBO_2294(arnT)
SBC: 
SDY: 
SDY_2453(arnT)
SDZ: 
ECA: 
ECA3142(arnT)
PCT: 
PCC: 
PWA: 
PEC: 
ETA: 
ETA_23790(arnT)
EPY: 
EpC_25140(arnT)
EPR: 
EAM: 
EAMY_1087(yfbI)
EAY: 
EAM_1094(arnT)
EBI: 
EbC_12150(arnT)
ERJ: 
PLU: 
plu2656(arnT)
PAY: 
PAU_01879(arnT)
WBR: 
WGLp162(arnT)
WGL: 
SGL: 
SG0291(arnT) SG1841(arnT)
SOD: 
Sant_1034(arnT1) Sant_3567(arnT2)
ENT: 
ENC: 
ENO: 
ECLO: 
EEC: 
ENL: 
ESC: 
EAS: 
EAU: 
EAE: 
EAE_05680(arnT)
EAR: 
ENR: 
KPN: 
KPN_03843(arnT)
KPU: 
KP1_5180(arnT)
KPM: 
KPP: 
KPE: 
KPK_0270(arnT)
KPO: 
KPR: 
KPR_5074(arnT)
KPJ: 
KPI: 
KVA: 
KOX: 
KOX_05060(arnT)
KOE: 
CFD: 
SPE: 
Spro_2158(arnT)
SRR: 
SRL: 
SRY: 
SRS: 
SRA: 
SMAF: 
SMW: 
SLQ: 
SERR: 
SFO: 
PMR: 
PMI1047(arnT)
PMIB: 
EIC: 
ETR: 
ETAE_1295(arnT)
ETD: 
ETAF_1207(pqaB)
ETC: 
HDE: 
HDEF_0859(arnT)
SECT: 
SEHC: 
DDA: 
DDC: 
DDD: 
DZE: 
XBO: 
XBJ1_2532(arnT)
XNE: 
XNC1_1839(pbgE1)
PAM: 
PANA_4006(arnT)
PLF: 
PAJ: 
PAJ_p0003(arnT)
PAQ: 
PVA: 
PAO: 
RAH: 
RAQ: 
RAA: 
Q7S_14220(arnT)
PSI: 
S70_08185(arnT) S70_19375(arnT)
EBT: 
MMK: 
ROR: 
EBF: 
PAE: 
PA3556(arnT)
PAEV: 
PAU: 
PAP: 
PAG: 
PAF: 
PNC: 
PDK: 
PSG: 
PRP: 
PAEP: 
PAER: 
PAEM: 
PAEL: 
PAES: 
PAEU: 
PAEG: 
PAEC: 
PSB: 
Psyr_2693(arnT)
PSYR: 
PSP: 
PFL: 
PFL_3047(arnT_1) PFL_4461(arnT_2)
PPRC: 
PFO: 
PFS: 
PFLU3039(arnT) PFLU4004(arnT)
PFC: 
PflA506_2686(arnT_1) PflA506_3380(arnT_2)
PPZ: 
PDR: 
PSK: 
PKC: 
PKB_2816(arnT2) PKB_4723(arnT2)
SSE: 
Ssed_0922(arnT)
AHA: 
AHA_0988(arnT)
AHY: 
ASA: 
ASA_3311(arnT)
GSK: 
TPX: 
AMO: 
DOI: 
HHY: 
HSW: 
GFO: 
ZPR: 
DDF: 
DAP: 
CNI: 
FSI: 
SAAL: 
 » show all
Taxonomy
Reference
1  [PMID:11535604]
  Authors
Trent MS, Ribeiro AA, Lin S, Cotter RJ, Raetz CR
  Title
An inner membrane enzyme in Salmonella and Escherichia coli that transfers 4-amino-4-deoxy-L-arabinose to lipid A: induction on polymyxin-resistant mutants  and role of a novel lipid-linked donor.
  Journal
J. Biol. Chem. 276 (2001) 43122-31.
  Organism
Escherichia coli, Salmonella Typhimurium
  Sequence
[eco:b2257] [stm:STM2301]
Reference
2  [PMID:11535605]
  Authors
Trent MS, Ribeiro AA, Doerrler WT, Lin S, Cotter RJ, Raetz CR
  Title
Accumulation of a polyisoprene-linked amino sugar in polymyxin-resistant Salmonella typhimurium and Escherichia coli: structural characterization and transfer to lipid A in the periplasm.
  Journal
J. Biol. Chem. 276 (2001) 43132-44.
Reference
3  [PMID:11535603]
  Authors
Zhou Z, Ribeiro AA, Lin S, Cotter RJ, Miller SI, Raetz CR
  Title
Lipid A modifications in polymyxin-resistant Salmonella typhimurium: PMRA-dependent 4-amino-4-deoxy-L-arabinose, and phosphoethanolamine incorporation.
  Journal
J. Biol. Chem. 276 (2001) 43111-21.
Reference
4  [PMID:16226890]
  Authors
Bretscher LE, Morrell MT, Funk AL, Klug CS
  Title
Purification and characterization of the L-Ara4N transferase protein ArnT from Salmonella typhimurium.
  Journal
Protein. Expr. Purif. 46 (2006) 33-9.
  Organism
Salmonella Typhimurium
  Sequence
[stm:STM2301]
Reference
5  [PMID:19947657]
  Authors
Impellitteri NA, Merten JA, Bretscher LE, Klug CS
  Title
Identification of a functionally important loop in Salmonella typhimurium ArnT.
  Journal
Biochemistry. 49 (2010) 29-35.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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