protein geranylgeranyltransferase type I;
GGTase-I;
GGTaseI

Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)

geranylgeranyl-diphosphate:protein-cysteine geranyltransferase

geranylgeranyl diphosphate + protein-cysteine = S-geranylgeranyl-protein + diphosphate

geranylgeranyl diphosphate [CPD:C00353];
protein-cysteine [CPD:C20119]

This enzyme, along with protein farnesyltransferase (EC 2.5.1.58) and protein geranylgeranyltransferase type II (EC 2.5.1.60), constitutes the protein prenyltransferase family of enzymes. Catalyses the formation of a thioether linkage between the C-1 atom of the geranylgeranyl group and a cysteine residue fourth from the C-terminus of the protein. These protein acceptors have the C-terminal sequence CA1A2X, where the terminal residue, X, is preferably leucine; serine, methionine, alanine or glutamine makes the protein a substrate for EC 2.5.1.58. The enzymes are relaxed in specificity for A1, but cannot act if A2 is aromatic. Known targets of this enzyme include most gamma-subunits of heterotrimeric G proteins and Ras-related GTPases such as members of the Ras and Rac/Rho families. A zinc metalloenzyme. The Zn2+ is required for peptide, but not for isoprenoid, substrate binding.

1  [PMID:8621375]

Casey PJ, Seabra MC.

Protein prenyltransferases.

J. Biol. Chem. 271 (1996) 5289-92.

Homo sapiens [GN:hsa], Rattus norvegicus [GN:rno], Saccharomyces cerevisiae [GN:sce]

2  [PMID:9003382]

Zhang FL, Casey PJ.

Influence of metal ions on substrate binding and catalytic activity of mammalian protein geranylgeranyltransferase type-I.

Biochem. J. 320 ( Pt 3) (1996) 925-32.

Homo sapiens [GN:hsa], Rattus norvegicus [GN:rno]

3

Gibbs, R.A.

Prenyl transfer and the enzymes of terpenoid and steroid biosynthesis.

In: Sinnott, M. (Ed.), Comprehensive Biological Catalysis. A Mechanistic Reference, vol. 1, Academic Press, San Diego, CA, 1998, p. 31-118.