KEGG   ENZYME: 2.5.1.81Help
Entry
EC 2.5.1.81                 Enzyme                                 

Name
geranylfarnesyl diphosphate synthase;
FGPP synthase;
(all-E) geranylfarnesyl diphosphate synthase;
GFPS;
Fgs
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
BRITE hierarchy
Sysname
geranylgeranyl-diphosphate:isopentenyl-diphosphate transtransferase (adding 1 isopentenyl unit)
Reaction(IUBMB)
geranylgeranyl diphosphate + isopentenyl diphosphate = (2E,6E,10E,14E)-geranylfarnesyl diphosphate + diphosphate [RN:R07475]
Reaction(KEGG)
Substrate
geranylgeranyl diphosphate [CPD:C00353];
isopentenyl diphosphate [CPD:C00129]
Product
(2E,6E,10E,14E)-geranylfarnesyl diphosphate;
diphosphate [CPD:C00013]
Comment
The enzyme from Methanosarcina mazei is involved in biosynthesis of the polyprenyl side-chain of methanophenazine, an electron carrier utilized for methanogenesis. It prefers geranylgeranyl diphosphate and farnesyl diphosphate as allylic substrate [1]. The enzyme from Aeropyrum pernix prefers farnesyl diphosphate as allylic substrate. The enzyme is involved in the biosynthesis of C25-C25 membrane lipids [2].
History
EC 2.5.1.81 created 2010
Reference
1  [PMID:20097171]
  Authors
Ogawa T, Yoshimura T, Hemmi H
  Title
Geranylfarnesyl diphosphate synthase from Methanosarcina mazei: Different role, different evolution.
  Journal
Biochem. Biophys. Res. Commun. 393 (2010) 16-20.
  Sequence
[up:Q8PYS1]
Reference
2  [PMID:10632701]
  Authors
Tachibana A, Yano Y, Otani S, Nomura N, Sako Y, Taniguchi M
  Title
Novel prenyltransferase gene encoding farnesylgeranyl diphosphate synthase from a hyperthermophilic archaeon, Aeropyrum pernix. Molecularevolution with alteration  in product specificity.
  Journal
Eur. J. Biochem. 267 (2000) 321-8.
  Sequence
[up:Q9UWR6]
Reference
3  [PMID:8137956]
  Authors
Tachibana A
  Title
A novel prenyltransferase, farnesylgeranyl diphosphate synthase, from the haloalkaliphilic archaeon, Natronobacterium pharaonis.
  Journal
FEBS. Lett. 341 (1994) 291-4.
  Sequence
[up:Q3IPL1]
Reference
4  [PMID:15548566]
  Authors
Lee PC, Mijts BN, Petri R, Watts KT, Schmidt-Dannert C
  Title
Alteration of product specificity of Aeropyrum pernix farnesylgeranyl diphosphate synthase (Fgs) by directed evolution.
  Journal
Protein. Eng. Des. Sel. 17 (2004) 771-7.
  Sequence
[up:Q9UWR6]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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