KEGG   ENZYME: 2.7.1.78Help
Entry
EC 2.7.1.78                 Enzyme                                 

Name
polynucleotide 5'-hydroxyl-kinase;
ATP:5'-dephosphopolynucleotide 5'-phosphatase;
PNK;
polynucleotide 5'-hydroxyl kinase (phosphorylating);
5'-hydroxyl polynucleotide kinase;
5'-hydroxyl polyribonucleotide kinase;
5'-hydroxyl RNA kinase;
DNA 5'-hydroxyl kinase;
DNA kinase;
polynucleotide kinase;
polynucleotide 5'-hydroxy-kinase
Class
Transferases;
Transferring phosphorus-containing groups;
Phosphotransferases with an alcohol group as acceptor
BRITE hierarchy
Sysname
ATP:5'-dephosphopolynucleotide 5'-phosphotransferase
Reaction(IUBMB)
ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA [RN:R03840]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
5'-dephospho-DNA [CPD:C02374]
Product
ADP [CPD:C00008];
5'-phospho-DNA [CPD:C02128]
Comment
Also acts on 5'-dephospho-RNA 3'-mononucleotides.
History
EC 2.7.1.78 created 1972
Orthology
K08073  bifunctional polynucleotide phosphatase/kinase
K14399  polyribonucleotide 5'-hydroxyl-kinase
Genes
HSA: 10978(CLP1) 11284(PNKP)
PTR: 451198(CLP1) 747683(PNKP)
PPS: 100980511(PNKP) 100994100(CLP1)
GGO: 101143287(CLP1) 101152889(PNKP)
PON: 100446807(PNKP) 100460422(CLP1)
NLE: 100584876(CLP1) 100587753(PNKP) 105740322
MCC: 705071(CLP1) 719084(PNKP)
MCF: 102133691(CLP1) 102140873(PNKP)
CSAB: 103235058(PNKP) 103235350(CLP1)
RRO: 104667214(PNKP) 104677688(CLP1)
RBB: 108514865(CLP1) 108535816(PNKP)
CJC: 100386802(CLP1) 100393151(PNKP)
SBQ: 101034804(PNKP) 101048258(CLP1)
MMU: 59047(Pnkp) 98985(Clp1)
RNO: 308576(Pnkp) 311166(Clp1)
CGE: 100755872(Clp1) 100767996(Pnkp)
NGI: 103736636(Clp1) 103743682(Pnkp)
HGL: 101712591(Pnkp) 101723956(Clp1)
CCAN: 109683911(Pnkp) 109688983(Clp1)
OCU: 100350312(CLP1)
TUP: 102486675(PNKP) 102490551(CLP1)
CFA: 484373(PNKP) 610161(CLP1)
AML: 100465832(PNKP) 100478336(CLP1)
UMR: 103657264(PNKP) 103666119(CLP1)
ORO: 101365436(CLP1) 101365837(PNKP)
FCA: 101091047(CLP1) 101093816(PNKP)
PTG: 102960670(CLP1) 102970079(PNKP)
AJU: 106979564(CLP1) 106983117(PNKP)
BTA: 506507(CLP1) 616621(PNKP)
BOM: 102264513(PNKP) 102277848(CLP1)
BIU: 109569885(CLP1) 109572131(PNKP)
PHD: 102332188(PNKP) 102333022(CLP1)
CHX: 102171898(CLP1) 102186043(PNKP)
OAS: 101106031(CLP1) 101114209(PNKP)
SSC: 100520541(CLP1) 100522442(PNKP)
CFR: 102515013(CLP1) 102516230(PNKP)
CDK: 105100187(PNKP) 105101224(CLP1)
BACU: 103001420(PNKP) 103006007(CLP1)
LVE: 103075581(PNKP) 103089865(CLP1)
OOR: 101280708(CLP1) 101290195(PNKP)
ECB: 100052218(PNKP) 100066955(CLP1)
EPZ: 103558224(CLP1) 103558284(PNKP)
EAI: 106822557(CLP1) 106824173(PNKP)
HAI: 109371620(CLP1) 109391028(PNKP)
RSS: 109436669(CLP1) 109452522(PNKP)
PALE: 102890706(CLP1) 102898132(PNKP)
LAV: 100657486(CLP1) 100672018(PNKP)
MDO: 100017655 100022871(CLP1) 100030171(PNKP)
SHR: 100928810(PNKP) 100931522(CLP1)
OAA: 100088944(PNKP) 100092173
GGA: 423131(CLP1)
MGP: 100547570(CLP1)
CJO: 107307754(PNKP) 107314449(CLP1)
APLA: 101799689(CLP1)
ACYG: 106033820(CLP1)
TGU: 100220989(CLP1)
GFR: 102032848(CLP1)
FAB: 101819677(CLP1)
PHI: 102107142(PNKP) 102108463(CLP1)
PMAJ: 107205510(CLP1)
FPG: 101916434(CLP1) 101924800(PNKP)
FCH: 102050209(PNKP) 102056567(CLP1)
CLV: 102091765(CLP1)
EGZ: 104129563(CLP1)
AAM: 106489148(CLP1)
ASN: 102372510(PNKP) 102384463(CLP1)
AMJ: 102558202(PNKP) 102571624(CLP1)
PSS: 102462947(CLP1)
CMY: 102932686(CLP1)
CPIC: 101933800(CLP1) 101940494(PNKP)
ACS: 100556487(clp1) 100558808(pnkp)
PVT: 110078286(CLP1) 110086941(PNKP)
GJA: 107110661(CLP1) 107119268(PNKP)
XLA: 100137702(pnkp.S) 431824(clp1.L)
XTR: 100145800(pnkp) 493364(clp1)
NPR: 108788104(CLP1) 108792103(PNKP)
DRE: 565621(clp1) 569462(pnkp)
IPU: 108268688(clp1) 108278314(pnkp)
AMEX: 103026644(pnkp) 103032393(clp1)
LCO: 104930443(pnkp)
NCC: 104951307(clp1) 104963321(pnkp)
MZE: 101480033(pnkp) 101487618(clp1)
OLA: 101158455(clp1) 101167386(pnkp)
XMA: 102216608(clp1) 102228822(pnkp)
PRET: 103471362(clp1) 103474841(pnkp)
NFU: 107374549(pnkp) 107376815(clp1)
LCF: 108885554(clp1) 108890636(pnkp)
HCQ: 109524444(clp1) 109529349(pnkp)
ELS: 105011239(pnkp) 105028688(clp1)
SFM: 108928645(pnkp) 108935929(clp1)
LCM: 102364593(PNKP) 102366070(CLP1)
CIN: 100182223 100183747(clp1)
DSI: Dsimw501_GD20873(Dsim_GD20873) Dsimw501_GD25757(Dsim_GD25757)
AGA: AgaP_AGAP007701(CLP1_ANOGA)
AME: 551305(cbc) 552254
CEL: CELE_F21D5.5(F21D5.5) CELE_F59A2.4(clpf-1)
ATH: AT3G04680(CLPS3) AT3G14890 AT5G39930(CLPS5)
LJA: Lj0g3v0096039.1(Lj0g3v0096039.1) Lj3g3v0427320.1(Lj3g3v0427320.1) Lj5g3v2290070.1(Lj5g3v2290070.1) Lj5g3v2290070.2(Lj5g3v2290070.2) Lj5g3v2290070.3(Lj5g3v2290070.3)
DOSA: Os01t0737300-00(Os01g0737300) Os02t0217500-01(Os02g0217500)
ATS: 109748167(LOC109748167) 109778679(LOC109778679)
ZMA: 100284798 541878(dnp2)
PDA: 103709610
SCE: YOR250C(CLP1)
ERC: Ecym_2382
KMX: KLMA_30448(CLP1)
NCS: NCAS_0B01210(NCAS0B01210)
NDI: NDAI_0E01090(NDAI0E01090)
TPF: TPHA_0D01350(TPHA0D01350)
TBL: TBLA_0D01320(TBLA0D01320)
TDL: TDEL_0A06350(TDEL0A06350)
KAF: KAFR_0A03930(KAFR0A03930)
CAL: CAALFM_C303840CA(CaO19.6931)
CAUR: QG37_00792
SLB: AWJ20_420(CLP1)
NTE: NEUTE1DRAFT103221(NEUTE1DRAFT_103221) NEUTE1DRAFT79143(NEUTE1DRAFT_79143)
MAW: MAC_01725
BFU: BCIN_12g03610(Bcclp1)
ANG: ANI_1_612164(An18g04570) ANI_1_818164(An18g06170)
ABE: ARB_00183(pnk1) ARB_03056
TVE: TRV_03023
ABP: AGABI1DRAFT104404(AGABI1DRAFT_104404) AGABI1DRAFT125354(AGABI1DRAFT_125354)
ABV: AGABI2DRAFT115344(AGABI2DRAFT_115344) AGABI2DRAFT176420(AGABI2DRAFT_176420)
MGL: MGL_1095
DDI: DDB_G0281229(PNKP) DDB_G0285507(clp1)
EHI: EHI_008100(55.t00023) EHI_148430(1.t00112)
PYO: PY17X_0709400(PY04248) PY17X_1140600(PY05803)
PCB: PCHAS_071830(PC000337.04.0) PCHAS_113870
TPV: TP01_0091
BBO: BBOV_IV000690(21.m03011) BBOV_IV008810(23.m06130)
CPV: cgd5_2880
SMIN: v1.2.015573.t1(symbB.v1.2.015573.t1) v1.2.022936.t1(symbB.v1.2.022936.t1)
 » show all
Taxonomy
Reference
1  [PMID:4287929]
  Authors
Novogrodsky A, Hurwitz J.
  Title
The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. I. Phosphorylation at 5'-hydroxyl termini.
  Journal
J. Biol. Chem. 241 (1966) 2923-32.
Reference
2  [PMID:4287930]
  Authors
Novogrodsky A, Tal M, Traub A, Hurwitz J.
  Title
The enzymatic phosphorylation of ribonucleic acid and deoxyribonucleic acid. II. Further properties of the 5'-hydroxyl polynucleotide kinase.
  Journal
J. Biol. Chem. 241 (1966) 2933-43.
Other DBs
ExplorEnz - The Enzyme Database: 2.7.1.78
IUBMB Enzyme Nomenclature: 2.7.1.78
ExPASy - ENZYME nomenclature database: 2.7.1.78
BRENDA, the Enzyme Database: 2.7.1.78
CAS: 37211-65-7

KEGG   ENZYME: 3.1.3.32Help
Entry
EC 3.1.3.32                 Enzyme                                 

Name
polynucleotide 3'-phosphatase;
2'(3')-polynucleotidase;
DNA 3'-phosphatase;
deoxyribonucleate 3'-phosphatase;
5'-polynucleotidekinase 3'-phosphatase
Class
Hydrolases;
Acting on ester bonds;
Phosphoric-monoester hydrolases
BRITE hierarchy
Sysname
polynucleotide 3'-phosphohydrolase
Reaction(IUBMB)
a 3'-phosphopolynucleotide + H2O = a polynucleotide + phosphate [RN:R02248]
Reaction(KEGG)
Substrate
3'-phosphopolynucleotide [CPD:C03463];
H2O [CPD:C00001]
Product
polynucleotide [CPD:C00419];
phosphate [CPD:C00009]
Comment
Also hydrolyses nucleoside 2'-, 3'- and 5'-monophosphates, but only 2'- and 3'-phosphopolynucleotides.
History
EC 3.1.3.32 created 1972
Orthology
K08073  bifunctional polynucleotide phosphatase/kinase
K08075  polynucleotide 3'-phosphatase
Genes
HSA: 11284(PNKP)
PTR: 747683(PNKP)
PPS: 100980511(PNKP)
GGO: 101152889(PNKP)
PON: 100446807(PNKP)
NLE: 100587753(PNKP)
MCC: 719084(PNKP)
MCF: 102140873(PNKP)
CSAB: 103235058(PNKP)
RRO: 104667214(PNKP)
RBB: 108535816(PNKP)
CJC: 100393151(PNKP)
SBQ: 101034804(PNKP)
MMU: 59047(Pnkp)
RNO: 308576(Pnkp)
CGE: 100767996(Pnkp)
NGI: 103743682(Pnkp)
HGL: 101712591(Pnkp)
CCAN: 109683911(Pnkp)
TUP: 102486675(PNKP)
CFA: 484373(PNKP)
AML: 100465832(PNKP)
UMR: 103657264(PNKP)
ORO: 101365837(PNKP)
FCA: 101093816(PNKP)
PTG: 102970079(PNKP)
AJU: 106983117(PNKP)
BTA: 616621(PNKP)
BOM: 102264513(PNKP)
BIU: 109572131(PNKP)
PHD: 102332188(PNKP)
CHX: 102186043(PNKP)
OAS: 101114209(PNKP)
SSC: 100522442(PNKP)
CFR: 102516230(PNKP)
CDK: 105100187(PNKP)
BACU: 103001420(PNKP)
LVE: 103075581(PNKP)
OOR: 101290195(PNKP)
ECB: 100052218(PNKP)
EPZ: 103558284(PNKP)
EAI: 106824173(PNKP)
MYB: 102244092(PNKP)
MYD: 102768538(PNKP)
HAI: 109391028(PNKP)
RSS: 109452522(PNKP)
PALE: 102898132(PNKP)
LAV: 100672018(PNKP)
TMU: 101345557
MDO: 100030171(PNKP)
SHR: 100928810(PNKP)
OAA: 100088944(PNKP)
CJO: 107307754(PNKP)
PHI: 102107142(PNKP)
FPG: 101924800(PNKP)
FCH: 102050209(PNKP)
ASN: 102372510(PNKP)
AMJ: 102558202(PNKP)
CPIC: 101940494(PNKP)
ACS: 100558808(pnkp)
PVT: 110086941(PNKP)
GJA: 107119268(PNKP)
XLA: 100137702(pnkp.S)
XTR: 100145800(pnkp)
NPR: 108792103(PNKP)
DRE: 569462(pnkp)
IPU: 108278314(pnkp)
AMEX: 103026644(pnkp)
LCO: 104930443(pnkp)
NCC: 104963321(pnkp)
MZE: 101480033(pnkp)
OLA: 101167386(pnkp)
XMA: 102228822(pnkp)
PRET: 103474841(pnkp)
NFU: 107374549(pnkp)
LCF: 108890636(pnkp)
HCQ: 109529349(pnkp)
ELS: 105011239(pnkp)
SFM: 108928645(pnkp)
LCM: 102364593(PNKP)
CIN: 100182223
DSI: Dsimw501_GD20873(Dsim_GD20873)
MDE: 101899475
AAG: 5563694
AME: 552254
BIM: 100748878
BTER: 100642258
SOC: 105201721
AEC: 105153315
ACEP: 105621630
PBAR: 105426415
HST: 105189063
CFO: 105253189
LHU: 105676740
NVI: 100123360
TCA: 658810
DPA: 109536367
NVL: 108565444
BMOR: 101745006
PMAC: 106717833
PRAP: 110993092
PXY: 105381652
ZNE: 110832645
FCD: 110855993
TUT: 107365023
CEL: CELE_F21D5.5(F21D5.5)
CBR: CBG16432
BMY: Bm1_40730
OBI: 106871510
LAK: 106157850
SHX: MS3_05085
EPA: 110235535
ADF: 107336894
HMG: 100214947
ATH: AT3G14890
CRB: 17890579
THJ: 104822566
CPAP: 110812493
CIT: 102622568
TCC: 18594227
GRA: 105777361
DZI: 111275921
EGR: 104448543
VRA: 106755703
VAR: 108339615
CCAJ: 109818171
CAM: 101515758
LJA: Lj0g3v0096039.1(Lj0g3v0096039.1) Lj3g3v0427320.1(Lj3g3v0427320.1)
FVE: 105350457
PPER: 18779538
PMUM: 103326113
PAVI: 110758176
ZJU: 107412932
CSV: 101207352
CMO: 103501815
MCHA: 111005189
CMAX: 111476896
CMOS: 111443571
CPEP: 111796745
RCU: 8275910
JCU: 105637019
HBR: 110670378
POP: 18095483
JRE: 109012260
VVI: 100243613
SLY: 101244972
SPEN: 107026395
SOT: 102582395
CANN: 107878589
NSY: 104245011
NTO: 104095112
INI: 109150619
SIND: 105176934
OEU: 111395567
LSV: 111893408
DCR: 108222650
BVG: 104891387
SOE: 110796091
NNU: 104593444
OSA: 4327876
DOSA: Os01t0737300-00(Os01g0737300)
OBR: 102705386
BDI: 100844676
ATS: 109778679(LOC109778679)
ZMA: 541878(dnp2)
SITA: 101776110
EGU: 105047531
MUS: 103988832
DCT: 110116554
AOF: 109843965
ATR: 18432740
PPP: 112283208
MNG: MNEG_4476
APRO: F751_0358
SCE: YMR156C(TPP1)
NCS: NCAS_0D01010(NCAS0D01010)
TPF: TPHA_0G01930(TPHA0G01930)
PIC: PICST_44837(TPP1)
NCR: NCU08151
NTE: NEUTE1DRAFT103221(NEUTE1DRAFT_103221)
MGR: MGG_09734
MAW: MAC_01725
MAJ: MAA_05467
CMT: CCM_08596
MBE: MBM_08017
ANI: AN5751.2
ANG: ANI_1_818164(An18g06170)
ABE: ARB_00183(pnk1)
PTE: PTT_12577
SPO: SPAC23C11.04c(pnk1)
CNE: CNA07870
CNB: CNBA7710
ABP: AGABI1DRAFT125354(AGABI1DRAFT_125354)
ABV: AGABI2DRAFT115344(AGABI2DRAFT_115344)
DDI: DDB_G0281229(PNKP)
EHI: EHI_148430(1.t00112)
PYO: PY17X_1140600(PY05803)
TAN: TA20015
TPV: TP01_0091
BBO: BBOV_IV000690(21.m03011)
SMIN: v1.2.015573.t1(symbB.v1.2.015573.t1)
SPAR: SPRG_11939
 » show all
Taxonomy
Reference
1  [PMID:4289819]
  Authors
Becker A, Hurwitz J.
  Title
The enzymatic cleavage of phosphate termini from polynucleotides.
  Journal
J. Biol. Chem. 242 (1967) 936-50.
Other DBs
ExplorEnz - The Enzyme Database: 3.1.3.32
IUBMB Enzyme Nomenclature: 3.1.3.32
ExPASy - ENZYME nomenclature database: 3.1.3.32
BRENDA, the Enzyme Database: 3.1.3.32
CAS: 37288-16-7

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