KEGG   ENZYME: 2.7.11.18Help
Entry
EC 2.7.11.18                Enzyme                                 
Name
myosin-light-chain kinase;
[myosin-light-chain] kinase;
ATP:myosin-light-chain O-phosphotransferase;
calcium/calmodulin-dependent myosin light chain kinase;
MLCK;
MLCKase;
myosin kinase;
myosin light chain kinase;
myosin light chain protein kinase;
myosin light-chain kinase (phosphorylating);
smooth-muscle-myosin-light-chain kinase;
STK18
Class
Transferases;
Transferring phosphorus-containing groups;
Protein-serine/threonine kinases
BRITE hierarchy
Sysname
ATP:[myosin light chain] O-phosphotransferase
Reaction(IUBMB)
ATP + [myosin light chain] = ADP + [myosin light chain] phosphate [RN:R03150]
Reaction(KEGG)
Substrate
ATP [CPD:C00002];
myosin light chain [CPD:C01003]
Product
ADP [CPD:C00008];
myosin light chain phosphate [CPD:C03875]
Comment
Requires Ca2+ and calmodulin for activity. The 20-kDa light chain from smooth muscle myosin is phosphorylated more rapidly than any other acceptor, but light chains from other myosins and myosin itself can act as acceptors, but more slowly.
Orthology
K00907  
myosin-light-chain kinase
Genes
HSA: 
340156(MYLK4) 4638(MYLK) 85366(MYLK2) 91807(MYLK3)
PTR: 
101057233(MYLK) 460640(MYLK) 465143(MYLK3) 471840(MYLK4) 742591(MYLK2)
PPS: 
100974667(MYLK3) 100978891(MYLK2) 100979422(MYLK) 100995666(MYLK4)
GGO: 
101142619(MYLK3) 101143745(MYLK2) 101151517(MYLK) 101151667(MYLK4)
PON: 
100171951(MYLK3) 100444714(MYLK4) 100452913(MYLK) 100453783(MYLK2)
MCC: 
707361(MYLK4) 712830 715422(MYLK) 716132(MYLK3)
MMU: 
107589(Mylk) 213435(Mylk3) 228785(Mylk2) 238564(Mylk4)
RNO: 
100911165 117558(Mylk2) 288057(Mylk) 291926(Mylk3)
CFA: 
475345(MYLK3) 477187(MYLK2) 488012(MYLK) 488190(MYLK4)
AML: 
FCA: 
101088408(MYLK4) 101091744(MYLK3) 101095813(MYLK2) 494213
BTA: 
338037(MYLK) 518025(MYLK4) 526379(MYLK3) 533378(MYLK2)
SSC: 
100157526 100524288(MYLK2) 396848(MYLK)
ECB: 
100050092(MYLK4) 100053698(MYLK2) 100056559(MYLK3) 100070482(MYLK)
MDO: 
100010869 100015876 100019648(MYLK) 100021377
SHR: 
100915288(MYLK2) 100925131(MYLK3) 100930073(MYLK)
OAA: 
100079091 100091283 100092442
GGA: 
396356(MYLK2) 396445(MYLK) 415748(MYLK3) 420893(MYLK4) 428278
MGP: 
100538449 100543918 100547989(MYLK) 100551067
TGU: 
100222880 100226590(MYLK) 100232455(MYLK3)
ACS: 
100557929 100559335 100567803
XTR: 
100489678 100497991 100498243
DRE: 
449707(mylk4b) 492576(mylkb) 558685(mlck2) 561635(mylk3) 566845 569510(mylka)
TRU: 
101061699 101062352 101070888 101072200
OLA: 
101163967 101167024 101171778
BFO: 
BRAFLDRAFT_154222 BRAFLDRAFT_201108 BRAFLDRAFT_221353 BRAFLDRAFT_69337
CIN: 
100176083 100177233
SPU: 
581963
DME: 
Dmel_CG42347(sqa)
DPO: 
Dpse_GA24209
DAN: 
Dana_GF11483
DER: 
Dere_GG22319 Dere_GG25266
DPE: 
Dper_GL17323
DSE: 
Dsec_GM20584
DSI: 
Dsim_GD10055 Dsim_GD25583
DWI: 
Dwil_GK15618
DYA: 
Dyak_GE14115 Dyak_GE22105
DGR: 
Dgri_GH22850
DMO: 
Dmoj_GI21229
DVI: 
Dvir_GJ20832
AGA: 
AgaP_AGAP002154 AgaP_AGAP006746 AgaP_AGAP010632
AAG: 
AaeL_AAEL005559 AaeL_AAEL007632 AaeL_AAEL008057
CQU: 
CpipJ_CPIJ005740 CpipJ_CPIJ012235 CpipJ_CPIJ012863
AME: 
413190
NVI: 
100118156(NV17133) 100118913(NV15546)
TCA: 
659444
API: 
100163829
PHU: 
Phum_PHUM123630
ISC: 
IscW_ISCW020494
CEL: 
CELE_ZC373.4(ZC373.4)
CBR: 
CBG01967
BMY: 
Bm1_37415
NVE: 
NEMVE_v1g61545
TAD: 
TRIADDRAFT_22755
 » show all
Taxonomy
Reference
1  [PMID:6894756]
  Authors
Adelstein RS, Klee CB.
  Title
Purification and characterization of smooth muscle myosin light chain kinase.
  Journal
J. Biol. Chem. 256 (1981) 7501-9.
  Organism
Meleagris gallopavo
Reference
2  [PMID:156362]
  Authors
Hathaway DR, Adelstein RS.
  Title
Human platelet myosin light chain kinase requires the calcium-binding protein calmodulin for activity.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 76 (1979) 1653-7.
  Organism
Homo sapiens [GN:hsa]
Reference
3  [PMID:4853304]
  Authors
Pires E, Perry SV, Thomas MA.
  Title
Myosin light-chain kinase, a new enzyme from striated muscle.
  Journal
FEBS. Lett. 41 (1974) 292-6.
  Organism
Oryctolagus cuniculus
Reference
4  [PMID:3881420]
  Authors
Nunnally MH, Rybicki SB, Stull JT.
  Title
Characterization of chicken skeletal muscle myosin light chain kinase. Evidence for muscle-specific isozymes.
  Journal
J. Biol. Chem. 260 (1985) 1020-6.
  Organism
Gallus gallus [GN:gga]
Reference
5  [PMID:3897230]
  Authors
Edelman AM, Takio K, Blumenthal DK, Hansen RS, Walsh KA, Titani K, Krebs EG.
  Title
Characterization of the calmodulin-binding and catalytic domains in skeletal muscle myosin light chain kinase.
  Journal
J. Biol. Chem. 260 (1985) 11275-85.
  Organism
Oryctolagus cuniculus
Reference
6  [PMID:12390014]
  Authors
Mal TK, Skrynnikov NR, Yap KL, Kay LE, Ikura M.
  Title
Detecting protein kinase recognition modes of calmodulin by residual dipolar couplings in solution NMR.
  Journal
Biochemistry. 41 (2002) 12899-906.
  Organism
Gallus gallus [GN:gga]
Reference
7  [PMID:10231558]
  Authors
Sobieszek A.
  Title
Enzyme kinetic characterization of the smooth muscle myosin phosphorylating system: activation by calcium and calmodulin and possible inhibitory mechanisms of antagonists.
  Journal
Biochim. Biophys. Acta. 1450 (1999) 77-91.
  Organism
Meleagris gallopavo
Reference
8  [PMID:9054394]
  Authors
Sobieszek A, Borkowski J, Babiychuk VS.
  Title
Purification and characterization of a smooth muscle myosin light chain kinase-phosphatase complex.
  Journal
J. Biol. Chem. 272 (1997) 7034-41.
  Organism
Meleagris gallopavo
Reference
9  [PMID:10098974]
  Authors
Fujita K, Ye LH, Sato M, Okagaki T, Nagamachi Y, Kohama K.
  Title
Myosin light chain kinase from skeletal muscle regulates an ATP-dependent interaction between actin and myosin by binding to actin.
  Journal
Mol. Cell. Biochem. 190 (1999) 85-90.
  Organism
Gallus gallus [GN:gga]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
51845-53-5
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