KEGG   ENZYME: 3.1.1.77Help
Entry
EC 3.1.1.77                 Enzyme                                 

Name
acyloxyacyl hydrolase
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
BRITE hierarchy
Reaction(IUBMB)
3-(acyloxy)acyl group of bacterial toxin + H2O = 3-hydroxyacyl group of bacterial toxin + a fatty acid [RN:R05792]
Reaction(KEGG)
Substrate
3-(acyloxy)acyl group of bacterial toxin [CPD:C11547];
H2O [CPD:C00001]
Product
3-hydroxyacyl group of bacterial toxin [CPD:C11548];
fatty acid [CPD:C00162]
Comment
The substrate is lipid A on the reducing end of the toxic lipopolysaccharide (LPS) of Salmonella typhimurium and related organisms. It consists of diglucosamine, beta-D-GlcN-(1-> 6)-D-GlcN, attached by glycosylation on O-6 of its non-reducing residue, phosphorylated on O-4 of this residue and on O-1 of its potentially reducing residue. Both residues carry 3-(acyloxy)acyl groups on N-2 and O-3. The enzyme from human leucocytes detoxifies the lipid by hydrolysing the secondary acyl groups from O-3 of the 3-hydroxyacyl groups on the disaccharide (LPS). It also possesses a wide range of phospholipase and acyltransferase activities [e.g. EC 3.1.1.4 (phospholipase A2), EC 3.1.1.5 (lysophospholipase), EC 3.1.1.32 (phospholipase A1) and EC 3.1.1.52 (phosphatidylinositol deacylase)], hydrolysing diacylglycerol and phosphatidyl compounds, but not triacylglycerols. It has a preference for saturated C12-C16 acyl groups.
History
EC 3.1.1.77 created 2001
Orthology
K01065  
acyloxyacyl hydrolase
Genes
HSA: 
313(AOAH)
PTR: 
463352(AOAH)
PPS: 
100994136(AOAH)
GGO: 
101134942(AOAH)
PON: 
100456260(AOAH)
MCC: 
706291(AOAH)
MCF: 
102141551(AOAH)
MMU: 
27052(Aoah)
RNO: 
498757(Aoah)
CGE: 
100768833(Aoah)
HGL: 
101718171(Aoah)
TUP: 
102490537(AOAH)
CFA: 
482402(AOAH)
AML: 
100471433(AOAH)
FCA: 
101101376(AOAH)
PTG: 
102960989(AOAH)
BTA: 
768208(AOAH)
BOM: 
102282514(AOAH)
PHD: 
102327820(AOAH)
CHX: 
102189546(AOAH)
OAS: 
101115787(AOAH)
SSC: 
100522290(AOAH)
CFR: 
102523361(AOAH)
BACU: 
103014073(AOAH)
LVE: 
103075940(AOAH)
ECB: 
100055508(AOAH)
MYB: 
102260205(AOAH)
MYD: 
102763366(AOAH)
PALE: 
102893901(AOAH)
MDO: 
103104605(AOAH)
SHR: 
100921933(AOAH)
GGA: 
420737(AOAH)
TGU: 
100230185(AOAH)
FAB: 
101820983(AOAH)
PHI: 
102111626(AOAH)
APLA: 
101793271(AOAH)
FPG: 
101918125(AOAH)
FCH: 
102059809(AOAH)
CLV: 
102091359(AOAH)
ASN: 
102373583(AOAH)
AMJ: 
102570975(AOAH)
PSS: 
102453660(AOAH)
CMY: 
102943071(AOAH)
ACS: 
100554361(aoah)
PBI: 
103067406(AOAH)
XTR: 
100491792(aoah)
BFO: 
CIN: 
SPU: 
TCA: 
NVE: 
AQU: 
MBR: 
DDI: 
DPP: 
DFA: 
ACAN: 
TET: 
TBR: 
TCR: 
NGR: 
 » show all
Taxonomy
Reference
1  [PMID:2398058]
  Authors
Erwin AL, Munford RS.
  Title
Deacylation of structurally diverse lipopolysaccharides by human acyloxyacyl hydrolase.
  Journal
J. Biol. Chem. 265 (1990) 16444-9.
Reference
2  [PMID:1883828]
  Authors
Hagen FS, Grant FJ, Kuijper JL, Slaughter CA, Moomaw CR, Orth K, O'Hara PJ, Munford RS.
  Title
Expression and characterization of recombinant human acyloxyacyl hydrolase, a leukocyte enzyme that deacylates bacterial lipopolysaccharides.
  Journal
Biochemistry. 30 (1991) 8415-23.
  Sequence
[hsa:313]
Reference
3  [PMID:1577781]
  Authors
Munford RS, Hunter JP.
  Title
Acyloxyacyl hydrolase, a leukocyte enzyme that deacylates bacterial lipopolysaccharides, has phospholipase, lysophospholipase, diacylglycerollipase, and acyltransferase activities in vitro.
  Journal
J. Biol. Chem. 267 (1992) 10116-21.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
110277-64-0

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