KEGG   ENZYME: 3.1.1.79Help
Entry
EC 3.1.1.79                 Enzyme                                 

Name
hormone-sensitive lipase;
HSL
Class
Hydrolases;
Acting on ester bonds;
Carboxylic-ester hydrolases
BRITE hierarchy
Sysname
diacylglycerol acylhydrolase
Reaction(IUBMB)
(1) diacylglycerol + H2O = monoacylglycerol + a carboxylate [RN:R05209];
(2) triacylglycerol + H2O = diacylglycerol + a carboxylate [RN:R01369];
(3) monoacylglycerol + H2O = glycerol + a carboxylate [RN:R07293]
Reaction(KEGG)
Substrate
diacylglycerol [CPD:C00165];
H2O [CPD:C00001];
triacylglycerol [CPD:C00422];
monoacylglycerol [CPD:C01885]
Product
monoacylglycerol [CPD:C01885];
carboxylate [CPD:C00060];
diacylglycerol [CPD:C00165];
glycerol [CPD:C00116]
Comment
This enzyme is a serine hydrolase. Compared with other lipases, hormone-sensitive lipase has a uniquely broad substrate specificity. It hydrolyses all acylglycerols (triacylglycerol, diacylglycerol and monoacylglycerol) [2,3,4] as well as cholesteryl esters [2,4], steroid fatty acid esters [5], retinyl esters [6] and p-nitrophenyl esters [4,7]. It exhibits a preference for the 1- or 3-ester bond of its acylglycerol substrate compared with the 2-ester bond [8]. The enzyme shows little preference for the fatty acids in the triacylglycerol, although there is some increase in activity with decreasing chain length. The enzyme activity is increased in response to hormones that elevate intracellular levels of cAMP.
History
EC 3.1.1.79 created 2004
Orthology
K07188  
hormone-sensitive lipase
Genes
HSA: 
3991(LIPE)
PTR: 
456076(LIPE)
PPS: 
100975071(LIPE)
GGO: 
101145804(LIPE)
PON: 
100445800(LIPE)
MCF: 
102143368(LIPE)
MMU: 
16890(Lipe)
RNO: 
25330(Lipe)
CGE: 
100755300(Lipe)
HGL: 
101709284(Lipe)
TUP: 
102477502(LIPE)
CFA: 
403607(LIPE)
AML: 
100479605(LIPE)
FCA: 
751818(LIPE)
PTG: 
102952452(LIPE)
BTA: 
286879(LIPE)
BOM: 
PHD: 
102342245(LIPE)
CHX: 
SSC: 
397583(LIPE)
CFR: 
102520149(LIPE)
BACU: 
103006716(LIPE)
LVE: 
103074607(LIPE)
ECB: 
MYB: 
102247234(LIPE)
MYD: 
102764534(LIPE)
PALE: 
102889858(LIPE)
MDO: 
103104890(LIPE)
SHR: 
100926393(LIPE)
ASN: 
102386893(LIPE)
AMJ: 
102571969(LIPE)
PBI: 
103054092(LIPE)
XLA: 
100174796(lipe)
XTR: 
733899(lipe)
DRE: 
557893(lipeb)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
BFO: 
CIN: 
SPU: 
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
AGA: 
AAG: 
CQU: 
AME: 
413702(GB17559)
NVI: 
TCA: 
BMOR: 
API: 
PHU: 
ISC: 
CEL: 
CELE_C46C11.1(C46C11.1)
CBR: 
LOA: 
TSP: 
SMM: 
NVE: 
HMG: 
TAD: 
CME: 
GSL: 
MBR: 
TET: 
PIF: 
GTT: 
NGR: 
 » show all
Taxonomy
Reference
1  [PMID:10940339]
  Authors
Holm C, Osterlund T, Laurell H, Contreras JA.
  Title
Molecular mechanisms regulating hormone-sensitive lipase and lipolysis.
  Journal
Annu. Rev. Nutr. 20 (2000) 365-93.
  Organism
Rattus norvegicus
Reference
2  [PMID:7240206]
  Authors
Fredrikson G, Stralfors P, Nilsson NO, Belfrage P.
  Title
Hormone-sensitive lipase of rat adipose tissue. Purification and some properties.
  Journal
J. Biol. Chem. 256 (1981) 6311-20.
  Organism
Rattus norvegicus
Reference
3  [PMID:14169138]
  Authors
VAUGHAN M, BERGER JE, STEINBERG D.
  Title
HORMONE-SENSITIVE LIPASE AND MONOGLYCERIDE LIPASE  ACTIVITIES IN ADIPOSE TISSUE.
  Journal
J. Biol. Chem. 239 (1964) 401-9.
  Organism
Oryctolagus cuniculus
Reference
4  [PMID:8912675]
  Authors
Osterlund T, Danielsson B, Degerman E, Contreras JA, Edgren G, Davis RC, Schotz MC, Holm C.
  Title
Domain-structure analysis of recombinant rat hormone-sensitive lipase.
  Journal
Biochem. J. 319 ( Pt 2) (1996) 411-20.
  Organism
Rattus norvegicus
Reference
5  [PMID:3196730]
  Authors
Lee FT, Adams JB, Garton AJ, Yeaman SJ.
  Title
Hormone-sensitive lipase is involved in the hydrolysis of lipoidal derivatives of estrogens and other steroid hormones.
  Journal
Biochim. Biophys. Acta. 963 (1988) 258-64.
  Organism
Bos taurus
Reference
6  [PMID:9162045]
  Authors
Wei S, Lai K, Patel S, Piantedosi R, Shen H, Colantuoni V, Kraemer FB, Blaner WS.
  Title
Retinyl ester hydrolysis and retinol efflux from BFC-1beta adipocytes.
  Journal
J. Biol. Chem. 272 (1997) 14159-65.
  Organism
Bos taurus
Reference
7  [PMID:2794798]
  Authors
Tsujita T, Ninomiya H, Okuda H.
  Title
p-nitrophenyl butyrate hydrolyzing activity of hormone-sensitive lipase from bovine adipose tissue.
  Journal
J. Lipid. Res. 30 (1989) 997-1004.
  Organism
Bos taurus
Reference
8  [PMID:14725507]
  Authors
Yeaman SJ.
  Title
Hormone-sensitive lipase--new roles for an old enzyme.
  Journal
Biochem. J. 379 (2004) 11-22.
  Organism
Homo sapiens, Rattus norvegicus
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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