| Entry |
|
|---|
| Name |
acireductone synthase;
E1;
E-1 enolase-phosphatase |
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| Class |
Hydrolases;
Acting on ester bonds;
Phosphoric-monoester hydrolases
 |
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| Sysname |
5-(methylthio)-2,3-dioxopentyl-phosphate phosphohydrolase
(isomerizing) |
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| Reaction(IUBMB) |
(1) 5-(methylthio)-2,3-dioxopentyl phosphate + H2O =
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate (overall
reaction) [RN:R07395];
(2) (1a) 5-(methylthio)-2,3-dioxopentyl phosphate =
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate (probably
spontaneous) [RN:R07393];
(3) (1b) 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate + H2O =
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate [RN:R07394] |
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| Reaction(KEGG) |
R07393 R07394 R07395
 |
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| Substrate |
5-(methylthio)-2,3-dioxopentyl phosphate [CPD:C15650];
H2O [CPD:C00001];
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate [CPD:C15651] |
|---|
| Product |
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one [CPD:C15606];
phosphate [CPD:C00009];
2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate [CPD:C15651] |
|---|
| Comment |
This bifunctional enzyme first enolizes the substrate to form the
intermediate 2-hydroxy-5-(methylthio)-3-oxopent-1-enyl phosphate,
which is then dephosphorylated to form the acireductone
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one [2]. The acireductone
represents a branch point in the methione-salvage pathway as it is
used in the formation of formate, CO and 3-(methylthio)propanoate by
EC 1.13.11.53 [acireductone dioxygenase (Ni2+-requiring)] and of
formate and 4-methylthio-2-oxobutanoate either by a spontaneous
reaction under aerobic conditions or by EC 1.13.11.54 {acireductone
dioxygenase [iron(II)-requiring]} [1,2]. |
|---|
| Pathway |
PATH: ec00270 Cysteine and methionine metabolism
PATH: ec01100 Metabolic pathways |
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| Orthology |
KO: K08965 2,3-diketo-5-methylthiopentyl-1-phosphate enolase
KO: K08966 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate
phosphatase
KO: K09880 enolase-phosphatase E1 |
|---|
| Genes |
HSA: 58478(ENOPH1)
PTR: 461214(ENOPH1)
MCC: 699388
MMU: 67870(Enoph1)
RNO: 305177(Enoph1)
CFA: 478452(ENOPH1)
BTA: 525563(ENOPH1)
ECB: 100060891
MDO: 100010409
OAA: 100082028
GGA: 422600(ENOPH1)
TGU: 100221564
XLA: 734408
XTR: 733933(enoph1)
DRE: 431773(enoph1)
CIN: 100175859
SPU: 589774
DME: Dmel_CG12173
DPO: Dpse_GA11453
DAN: Dana_GF16066
DER: Dere_GG11122
DPE: Dper_GL22195
DSE: Dsec_GM10653
DSI: Dsim_GD19634
DYA: Dyak_GE25301
DGR: Dgri_GH22720
DMO: Dmoj_GI10215
AGA: AgaP_AGAP003331
AAG: AaeL_AAEL000109
CQU: CpipJ_CPIJ009638
AME: 411809
NVI: 100117336
TCA: 664509
API: 100167644
CEL: F58H1.3
CBR: CBG23332
HMG: 100213757
POP: POPTR_827604
RCU: RCOM_0591060
VVI: 100257258
OSA: 4326460(Os01g0101200) 4350524(Os11g0484000)
SBI: SORBI_03g009140(SORBIDRAFT_03g009140)
ZMA: 100282341(IDP388)
PPP: PHYPADRAFT_111231 PHYPADRAFT_146721
SCE: YEL038W(UTR4)
AGO: AGOS_AFR359C
KLA: KLLA0E22198g
LTH: KLTH0C03388g
DHA: DEHA0B04070g
PIC: PICST_44113
PPA: PAS_chr2-2_0446
VPO: Kpol_1059p21
LEL: LELG_00943
ZRO: ZYRO0F05236g
CAL: CaO19.1495(UTR4)
CTP: CTRG_01277
CDU: CD36_16690
CGR: CAGL0L01287g
YLI: YALI0D16797g
SPO: SPAC644.08
NCR: NCU06228
PAN: PODANSg5285
MGR: MGG_10183
FGR: FG02491.1
BFU: BC1G_15439
MBR: MONBRDRAFT_26645
PTI: PHATRDRAFT_31147
YPG: YpAngola_A3330
YPP: YPDSF_2881
YPS: YPTB0875
YPI: YpsIP31758_3181
YPY: YPK_3320
YPB: YPTS_0916
YEN: YE3232(mntC)
ECA: ECA3486(masA)
PCT: PC1_3302
PWA: Pecwa_3456
ETA: ETA_26080(masA)
ENT: Ent638_1142
ESA: ESA_02725
CTU: Ctu_12370(mtnC)
KPN: KPN_00644
KPE: KPK_3930(mtnC)
KPU: KP1_1595(mtnC)
CKO: CKO_03972
SPE: Spro_0943
DDA: Dd703_0919
DDC: Dd586_3257
DZE: Dd1591_0886
XFA: XF2211
XFT: PD1260(masA)
XFM: Xfasm12_1412
XFN: XfasM23_1345
XCC: XCC1818
XCB: XC_2371
XCA: xccb100_2105
XCV: XCV1884
XAC: XAC1838
XOO: XOO2137
XOM: XOO_2006
XOP: PXO_00846
SML: Smlt2187(masA)
SMT: Smal_1780
PAE: PA1685(masA)
PAU: PA14_42720(masA)
PAP: PSPA7_3587(mtnC)
PAG: PLES_36421(masA)
PST: PSPTO_2047
PSB: Psyr_1857
PSP: PSPPH_1816
PFO: Pfl01_1726
AVN: Avin_23800
SON: SO_0084
SDN: Sden_0077
SFR: Sfri_3966
SAZ: Sama_0074
SBL: Sbal_4264
SBM: Shew185_0085
SBN: Sbal195_0091
SBP: Sbal223_0088
SLO: Shew_3771
SPC: Sputcn32_0069
SSE: Ssed_4454
SPL: Spea_4180
SHE: Shewmr4_0090
SHM: Shewmr7_0085
SHN: Shewana3_0089
SHW: Sputw3181_4008
SHL: Shal_0067
SWD: Swoo_4855
SWP: swp_0123
MAQ: Maqu_0909
MCA: MCA0797
TGR: Tgr7_2625
HCH: HCH_01846
ABO: ABO_1447
AFE: Lferr_0596 Lferr_0597
AFR: AFE_0434(ccbL2) AFE_0435
MEI: Msip34_1204
DAL: Dalk_2418
ADE: Adeh_0655
ACP: A2cp1_0690
AFW: Anae109_0700
ANK: AnaeK_0689
SCL: sce1551(masA) sce2820
PLA: Plav_1854
BBT: BBta_2512
XAU: Xaut_2913
GOX: GOX1244
GBE: GbCGDNIH1_2267
ACR: Acry_0019
GDI: GDI_0587
GDJ: Gdia_1413
APT: APA01_07360
BSU: BSU13590(ykrW) BSU13600(ykrX)
BAN: BA4255(mtnW) BA4256(mtnX)
BAR: GBAA4255(mtnW) GBAA4256(mtnX)
BAA: BA_4714 BA_4715
BAT: BAS3946(mtnW) BAS3947(mtnX)
BAH: BAMEG_4296(mtnW) BAMEG_4297(mtnX)
BAI: BAA_4278(mtnW) BAA_4279(mtnX)
BCE: BC4036(mtnW) BC4037(mtnX)
BCA: BCE_4103(mtnW) BCE_4104(mtnX)
BCZ: BCZK3793(mtnW) BCZK3794(mtnX)
BCR: BCAH187_A4167(mtnW) BCAH187_A4168(mtnX)
BCB: BCB4264_A4145(mtnW) BCB4264_A4146(mtnX)
BCU: BCAH820_4057(mtnW) BCAH820_4058(mtnX)
BCG: BCG9842_B1092(mtnX) BCG9842_B1093(mtnW)
BCQ: BCQ_3826(rbcL) BCQ_3827
BCX: BCA_4149(mtnW) BCA_4150(mtnX)
BCY: Bcer98_2735(mtnW) Bcer98_2736(mtnX)
BTK: BT9727_3778(mtnW) BT9727_3779(mtnX)
BTL: BALH_3653(mtnW) BALH_3654(mtnX)
BWE: BcerKBAB4_3865(mtnW) BcerKBAB4_3866(mtnX)
BLI: BL03540(mtnW) BL03541(mtnB)
BLD: BLi01515(mtnW) BLi01516(ykrY)
BCL: ABC1775(mtnW)
BAY: RBAM_013370(mtnW) RBAM_013380(mtnX)
BPU: BPUM_1252(mtnW) BPUM_1253(mtnX)
GKA: GK0953(mtnW) GK0954(mtnX)
GTN: GTNG_0841(mtnW) GTNG_0842(mtnX)
GWC: GWCH70_0850 GWCH70_0851
GYM: GYMC10_2266 GYMC10_2267
GYC: GYMC61_1745 GYMC61_1746
AFL: Aflv_1996(mtnX) Aflv_1997(mtnW)
LSP: Bsph_4351
ESI: Exig_0429(mtnX) Exig_0430(mtnW)
EAT: EAT1b_1436 EAT1b_1437
BBE: BBR47_48900(mtnW) BBR47_48910(mtnX)
PJD: Pjdr2_2464 Pjdr2_2465
AAC: Aaci_0096 Aaci_0097
NTH: Nther_2774
NFA: nfa45460
SMA: SAV_6660(masA)
FAL: FRAAL1409(masA)
NML: Namu_0013
SEN: SACE_3607(masA)
AMI: Amir_0985
LIL: LA0929
LIC: LIC12718
LBJ: LBJ_2207
LBL: LBL_2200
LBI: LEPBI_I2960
LBF: LBF_2858
EMI: Emin_0373
SYW: SYNW1964(masA)
SYC: syc1010_d syc2102_c(masA)
SYF: Synpcc7942_0510 Synpcc7942_1994
SYD: Syncc9605_0489
SYE: Syncc9902_1846
SYR: SynRCC307_0442 SynRCC307_0448(masA)
SYX: SynWH7803_0537(masA)
SYP: SYNPCC7002_A0552
MAR: MAE_13070 MAE_51120
CYP: PCC8801_1576 PCC8801_2072
CYC: PCC7424_0306 PCC7424_0958
CYH: Cyan8802_1601 Cyan8802_2096
PMT: PMT0184(masA)
PMF: P9303_21781
AAE: aq_1977(masA)
HYA: HY04AAS1_1512
SUL: SYO3AOP1_0245
SAF: SULAZ_1667(mtnC)
PMX: PERMA_0409 PERMA_0829(mtnC)
TTR: Tter_0864
AFU: AF1587(rbcL-1)
 |
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Reference
Authors
Title
Journal
Organism
|
1 [PMID:8227039]
Myers RW, Wray JW, Fish S, Abeles RH.
Purification and characterization of an enzyme involved in oxidative
carbon-carbon bond cleavage reactions in the methionine salvage
pathway of Klebsiella pneumoniae.
J. Biol. Chem. 268 (1993) 24785-91.
Klebsiella pneumoniae [GN:kpn] |
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Reference
Authors
Title
Journal
Organism
|
2 [PMID:7852397]
Wray JW, Abeles RH.
The methionine salvage pathway in Klebsiella pneumoniae and rat
liver. Identification and characterization of two novel
dioxygenases.
J. Biol. Chem. 270 (1995) 3147-53.
Rattus norvegicus [GN:rno], Klebsiella pneumoniae [GN:kpn] |
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Reference
Authors
Title
Journal
Organism
|
3 [PMID:15843022]
Wang H, Pang H, Bartlam M, Rao Z.
Crystal structure of human E1 enzyme and its complex with a
substrate analog reveals the mechanism of its phosphatase/enolase
activity.
J. Mol. Biol. 348 (2005) 917-26.
Homo sapiens [GN:hsa] |
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| Other DBs |
ExplorEnz - The Enzyme Database: 3.1.3.77
IUBMB Enzyme Nomenclature: 3.1.3.77
ExPASy - ENZYME nomenclature database: 3.1.3.77
BRENDA, the Enzyme Database: 3.1.3.77 |
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