KEGG   ENZYME: 3.1.4.50Help
Entry
EC 3.1.4.50                 Enzyme                                 

Name
glycosylphosphatidylinositol phospholipase D;
GPI-PLD;
glycoprotein phospholipase D;
phosphatidylinositol phospholipase D;
phosphatidylinositol-specific phospholipase D
Class
Hydrolases;
Acting on ester bonds;
Phosphoric-diester hydrolases
BRITE hierarchy
Sysname
glycoprotein-phosphatidylinositol phosphatidohydrolase
Reaction(IUBMB)
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + 3-sn-phosphatidate [RN:R06623]
Reaction(KEGG)
R06623(G);
(other) R04149 R07398
Show
Substrate
6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol [CPD:C04248];
H2O [CPD:C00001]
Product
6-(alpha-D-glucosaminyl)-1D-myo-inositol [CPD:C15658];
3-sn-phosphatidate [CPD:C00416]
Comment
This enzyme is also active when O-4 of the glucosamine is substituted by carrying the oligosaccharide that can link a protein to the structure. It therefore cleaves proteins from the lipid part of the glycosylphosphatidylinositol (GPI) anchors, but does so by hydrolysis, whereas glycosylphosphatidylinositol diacylglycerol-lyase (EC 4.6.1.14) does so by elimination. It acts on plasma membranes only after solubilization of the substrate with detergents or solvents, but it may act on intracellular membranes.
History
EC 3.1.4.50 created 1990, modified 2002
Pathway
Glycosylphosphatidylinositol(GPI)-anchor biosynthesis
Orthology
K01127  
glycosylphosphatidylinositol phospholipase D
Genes
HSA: 
2822(GPLD1)
PTR: 
462473(GPLD1)
PPS: 
100973223(GPLD1)
GGO: 
101146393(GPLD1)
PON: 
100455517(GPLD1)
MCC: 
707782(GPLD1)
MCF: 
102125729(GPLD1)
MMU: 
14756(Gpld1)
RNO: 
291132(Gpld1)
CGE: 
100759513(Gpld1)
HGL: 
101724062(Gpld1)
TUP: 
102501907(GPLD1)
CFA: 
478735(GPLD1)
AML: 
100484842(GPLD1)
FCA: 
101088486(GPLD1)
PTG: 
102964289(GPLD1)
BTA: 
287025(GPLD1)
BOM: 
102286788(GPLD1)
PHD: 
102336002(GPLD1)
CHX: 
102169718(GPLD1)
OAS: 
101119000(GPLD1)
SSC: 
100157497(GPLD1)
CFR: 
102518271(GPLD1)
BACU: 
103003582(GPLD1)
LVE: 
103088629(GPLD1)
ECB: 
100067517(GPLD1)
MYB: 
102260484(GPLD1)
MYD: 
102753404(GPLD1)
PALE: 
102896882(GPLD1)
MDO: 
100024296(GPLD1)
SHR: 
100931811(GPLD1)
OAA: 
100092985(GPLD1)
GGA: 
420819(GPLD1)
MGP: 
100546397(GPLD1)
APLA: 
101792700(GPLD1)
TGU: 
100230775(GPLD1)
FAB: 
101816045(GPLD1)
PHI: 
102114237(GPLD1)
FPG: 
101912228(GPLD1)
FCH: 
102052413(GPLD1)
CLV: 
102090900(GPLD1)
ASN: 
102375814(GPLD1)
AMJ: 
102574726(GPLD1)
PSS: 
102448848(GPLD1)
CMY: 
102944962(GPLD1)
ACS: 
100567850(gpld1)
PBI: 
103063314(GPLD1)
XTR: 
100493665(gpld1)
LCM: 
102345190(GPLD1)
BFO: 
SPU: 
NVE: 
SCE: 
KLA: 
LTH: 
ZRO: 
CGR: 
NCS: 
NCAS_0A09790(NCAS0A09790)
NDI: 
NDAI_0G06190(NDAI0G06190)
TBL: 
TBLA_0D05660(TBLA0D05660)
TDL: 
TDEL_0H01030(TDEL0H01030)
PPA: 
YLI: 
TML: 
DDI: 
DPP: 
DFA: 
DFA_04808(pldG)
ACAN: 
NOC: 
 » show all
Taxonomy
Reference
1  [PMID:3422494]
  Authors
Low MG, Prasad AR.
  Title
A phospholipase D specific for the phosphatidylinositol anchor of cell-surface proteins is abundant in plasma.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 85 (1988) 980-4.
Reference
2  [PMID:3028377]
  Authors
Malik AS, Low MG.
  Title
Conversion of human placental alkaline phosphatase from a high Mr form to a low Mr form during butanol extraction. An investigation of the role of endogenous phosphoinositide-specific phospholipases.
  Journal
Biochem. J. 240 (1986) 519-27.
Reference
3  [PMID:7961859]
  Authors
Li JY, Hollfelder K, Huang KS, Low MG.
  Title
Structural features of GPI-specific phospholipase D revealed by proteolytic fragmentation and Ca2+ binding studies.
  Journal
J. Biol. Chem. 269 (1994) 28963-71.
Reference
4  [PMID:11254757]
  Authors
Deeg MA, Bierman EL, Cheung MC.
  Title
GPI-specific phospholipase D associates with an apoA-I- and apoA-IV-containing complex.
  Journal
J. Lipid. Res. 42 (2001) 442-51.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
113756-14-2

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