KEGG   ENZYME: 3.2.1.152Help
Entry
EC 3.2.1.152                Enzyme                                 

Name
mannosylglycoprotein endo-beta-mannosidase;
endo-beta-mannosidase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of the alpha-D-mannosyl-(1->6)-beta-D-mannosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl sequence of glycoprotein to alpha-D-mannosyl-(1->6)-D-mannose and N-acetyl-beta-D-glucosaminyl-(1->4)-N-acetyl-beta-D-glucosaminyl sequences
Comment
The substrate group is a substituent on N-4 of an asparagine residue in the glycoprotein. The mannose residue at the non-reducing end of the sequence may carry further alpha-D-mannosyl groups on O-3 or O-6, but such a substituent on O-3 of the beta-D-mannosyl group prevents the action of the enzyme. The enzyme was obtained from the lily, Lilium longiflorum.
History
EC 3.2.1.152 created 2005
Orthology
K18577  
mannosylglycoprotein endo-beta-mannosidase
Genes
ATH: 
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
TCC: 
GMX: 
PVU: 
MTR: 
CAM: 
FVE: 
PPER: 
PMUM: 
MDM: 
CSV: 
CMO: 
RCU: 
POP: 
POPTR_0013s02210g(POPTRDRAFT_570830)
VVI: 
SLY: 
SOT: 
OSA: 
DOSA: 
Os05t0391500-01(Os05g0391500)
OBR: 
BDI: 
SBI: 
SORBI_09g019350(SORBIDRAFT_09g019350)
SITA: 
PDA: 
ATR: 
s00025p00243690(AMTR_s00025p00243690)
SMO: 
PPP: 
CSL: 
TPS: 
EHX: 
GTT: 
 » show all
Taxonomy
Reference
1  [PMID:15247239]
  Authors
Ishimizu T, Sasaki A, Okutani S, Maeda M, Yamagishi M, Hase S.
  Title
Endo-beta-mannosidase, a plant enzyme acting on N-glycan: purification, molecular cloning, and characterization.
  Journal
J. Biol. Chem. 279 (2004) 38555-62.
  Sequence
[ath:AT1G09010]
Reference
2  [PMID:9990135]
  Authors
Sasaki A, Yamagishi M, Mega T, Norioka S, Natsuka S, Hase S.
  Title
Partial purification and characterization of a novel endo-beta-mannosidase acting on N-linked sugar chains from Lilium longflorum thumb.
  Journal
J. Biochem. (Tokyo). 125 (1999) 363-7.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
141176-95-6

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