KEGG   ENZYME: 3.2.1.169Help
Entry
EC 3.2.1.169                Enzyme                                 

Name
protein O-GlcNAcase;
OGA;
glycoside hydrolase O-GlcNAcase;
O-GlcNAcase;
BtGH84;
O-GlcNAc hydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
[protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine/threonine N-acetylglucosaminyl hydrolase
Reaction(IUBMB)
(1) [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O = [protein]-L-serine + N-acetyl-D-glucosamine  [RN:R09672];
(2) [protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-theronine + H2O = [protein]-L-threonine + N-acetyl-D-glucosamine [RN:R09673]
Reaction(KEGG)
Substrate
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine [CPD:C19802];
H2O [CPD:C00001];
[protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-theronine [CPD:C19804]
Product
[protein]-L-serine [CPD:C06395];
N-acetyl-D-glucosamine [CPD:C00140];
[protein]-L-threonine [CPD:C19803]
Comment
Within higher eukaryotes post-translational modification of protein serines/threonines with N-acetylglucosamine (O-GlcNAc) is dynamic, inducible and abundant, regulating many cellular processes by interfering with protein phosphorylation. EC 2.4.1.255 (protein O-GlcNAc transferase) transfers GlcNAc onto substrate proteins and EC 3.2.1.169 (protein O-GlcNAcase) cleaves GlcNAc from the modified proteins.
History
EC 3.2.1.169 created 2011
Orthology
K15719  
protein O-GlcNAcase / histone acetyltransferase
Genes
HSA: 
10724(MGEA5)
PTR: 
450691(MGEA5)
PPS: 
100979884(MGEA5)
GGO: 
101124144(MGEA5)
PON: 
100459796(MGEA5)
MCC: 
712370(MGEA5)
MCF: 
102142813(MGEA5)
MMU: 
76055(Mgea5)
RNO: 
154968(Mgea5)
CGE: 
100751586(Mgea5)
HGL: 
101702515(Mgea5)
TUP: 
102491291(MGEA5)
CFA: 
477802(MGEA5)
AML: 
100472078(MGEA5)
FCA: 
101098493(MGEA5)
PTG: 
102959286(MGEA5)
BTA: 
538561(MGEA5)
BOM: 
102279409(MGEA5)
PHD: 
102316524(MGEA5)
CHX: 
102179540(MGEA5)
SSC: 
100152585(MGEA5)
CFR: 
102507074(MGEA5)
BACU: 
103001043(MGEA5)
LVE: 
103070168(MGEA5)
ECB: 
100060245(MGEA5)
MYB: 
102258473(MGEA5)
MYD: 
102763853(MGEA5)
PALE: 
102892734(MGEA5)
MDO: 
100023348(MGEA5)
SHR: 
100924785(MGEA5)
OAA: 
100086916(MGEA5)
GGA: 
423851(MGEA5)
MGP: 
TGU: 
100222791(MGEA5)
FAB: 
PHI: 
APLA: 
FPG: 
FCH: 
CLV: 
ASN: 
AMJ: 
102563356(MGEA5)
PSS: 
102451920(MGEA5)
CMY: 
102948301(MGEA5)
ACS: 
100559082(mgea5)
PBI: 
103061735(MGEA5)
XTR: 
100145437(mgea5)
DRE: 
324487(mgea5)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
CMK: 
103190567(mgea5)
BFO: 
CIN: 
SPU: 
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
AGA: 
AAG: 
CQU: 
AME: 
411905(GB19614)
NVI: 
TCA: 
BMOR: 
API: 
100163269(MGEA5)
PHU: 
ISC: 
LOA: 
SMM: 
HMG: 
AQU: 
ACAN: 
DPD: 
 » show all
Taxonomy
Reference
1  [PMID:11148210]
  Authors
Gao Y, Wells L, Comer FI, Parker GJ, Hart GW
  Title
Dynamic O-glycosylation of nuclear and cytosolic proteins: cloning and characterization of a neutral, cytosolic beta-N-acetylglucosaminidase from human  brain.
  Journal
J. Biol. Chem. 276 (2001) 9838-45.
  Organism
Homo sapiens
  Sequence
[hsa:10724]
Reference
2  [PMID:11788610]
  Authors
Wells L, Gao Y, Mahoney JA, Vosseller K, Chen C, Rosen A, Hart GW
  Title
Dynamic O-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic beta-N-acetylglucosaminidase, O-GlcNAcase.
  Journal
J. Biol. Chem. 277 (2002) 1755-61.
  Organism
Homo sapiens
  Sequence
[hsa:10724]
Reference
3  [PMID:16533067]
  Authors
Cetinbas N, Macauley MS, Stubbs KA, Drapala R, Vocadlo DJ
  Title
Identification of Asp174 and Asp175 as the key catalytic residues of human O-GlcNAcase by functional analysis of site-directed mutants.
  Journal
Biochemistry. 45 (2006) 3835-44.
Reference
4  [PMID:16565725]
  Authors
Dennis RJ, Taylor EJ, Macauley MS, Stubbs KA, Turkenburg JP, Hart SJ, Black GN, Vocadlo DJ, Davies GJ
  Title
Structure and mechanism of a bacterial beta-glucosaminidase having O-GlcNAcase activity.
  Journal
Nat. Struct. Mol. Biol. 13 (2006) 365-71.
  Organism
Bacteroides thetaiotaomicron
  Sequence
[up:Q89ZI2]
Reference
5  [PMID:16584714]
  Authors
Kim EJ, Kang DO, Love DC, Hanover JA
  Title
Enzymatic characterization of O-GlcNAcase isoforms using a fluorogenic GlcNAc substrate.
  Journal
Carbohydr. Res. 341 (2006) 971-82.
Reference
6  [PMID:8034696]
  Authors
Dong DL, Hart GW
  Title
Purification and characterization of an O-GlcNAc selective N-acetyl-beta-D-glucosaminidase from rat spleen cytosol.
  Journal
J. Biol. Chem. 269 (1994) 19321-30.
  Organism
Rattus norvegicus
  Sequence
[rno:154968]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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