KEGG   ENZYME: 3.2.1.182Help
Entry
EC 3.2.1.182                Enzyme                                 

Name
4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl glucoside beta-D-glucosidase;
DIMBOAGlc hydrolase;
DIMBOA glucosidase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside beta-D-glucosidase
Reaction(IUBMB)
(1) (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O = 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose;
(2) (2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside + H2O = 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one + D-glucose
Substrate
(2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside;
H2O [CPD:C00001];
(2R)-4-hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
Product
2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one [CPD:C04720];
D-glucose [CPD:C00031];
2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one [CPD:C15770]
Comment
The enzyme from Triticum aestivum (wheat) has a higher affinity for DIMBOA glucoside than DIBOA glucoside. With Secale cereale (rye) the preference is reversed.
History
EC 3.2.1.182 created 2012
Reference
1  [PMID:10750901]
  Authors
Sue M, Ishihara A, Iwamura H
  Title
Purification and characterization of a hydroxamic acid glucoside beta-glucosidase from wheat (Triticum aestivum L.) seedlings.
  Journal
Planta. 210 (2000) 432-8.
  Organism
Triticum aestivum
  Sequence
Reference
2  [PMID:10773341]
  Authors
Sue M, Ishihara A, Iwamura H
  Title
Purification and characterization of a beta-glucosidase from rye (Secale cereale  L.) seedlings.
  Journal
Plant. Sci. 155 (2000) 67-74.
  Organism
Secale cereale
  Sequence
[up:Q9FYS3]
Reference
3  [PMID:11106394]
  Authors
Czjzek M, Cicek M, Zamboni V, Bevan DR, Henrissat B, Esen A
  Title
The mechanism of substrate (aglycone) specificity in beta -glucosidases is revealed by crystal structures of mutant maize beta -glucosidase-DIMBOA, -DIMBOAGlc, and -dhurrin complexes.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 97 (2000) 13555-60.
  Organism
Zea mays
  Sequence
[up:P49235]
Reference
4
  Authors
Nikus, J., Esen, A. and Jonsson, L.M.V.
  Title
Cloning of a plastidic rye (Secale cereale) beta-glucosidase cDNA and its expression in Escherichia coli.
  Journal
Physiol. Plantarum 118 (2003) 337-348.
Reference
5  [PMID:16751439]
  Authors
Sue M, Yamazaki K, Yajima S, Nomura T, Matsukawa T, Iwamura H, Miyamoto T
  Title
Molecular and structural characterization of hexameric beta-D-glucosidases in wheat and rye.
  Journal
Plant. Physiol. 141 (2006) 1237-47.
  Organism
Secale cereale, Triticum aestivum
  Sequence
Reference
6  [PMID:21421370]
  Authors
Sue M, Nakamura C, Miyamoto T, Yajima S
  Title
Active-site architecture of benzoxazinone-glucoside beta-D-glucosidases in Triticeae.
  Journal
Plant. Sci. 180 (2011) 268-75.
  Organism
Secale cereale, Triticum aestivum
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system