KEGG   ENZYME: 3.2.1.62Help
Entry
EC 3.2.1.62                 Enzyme                                 
Name
glycosylceramidase;
phlorizin hydrolase;
phloretin-glucosidase;
glycosyl ceramide glycosylhydrolase;
cerebrosidase;
phloridzin beta-glucosidase;
lactase-phlorizin hydrolase;
phloridzin glucosidase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
glycosyl-N-acylsphingosine glycohydrolase
Reaction(IUBMB)
a glycosyl-N-acylsphingosine + H2O = a ceramide + a sugar [RN:R01501]
Reaction(KEGG)
Substrate
glycosyl-N-acylsphingosine [CPD:C03701];
H2O [CPD:C00001]
Product
ceramide [CPD:C00195];
sugar [CPD:C11477]
Comment
Broad specificity [cf. EC 3.2.1.45 (glucosylceramidase) and EC 3.2.1.46 (galactosylceramidase)]. Also hydrolyses phlorizin to phloretin and glucose. The intestinal enzyme is a complex that also catalyses the reaction of EC 3.2.1.108 lactase.
Orthology
K01229  
lactase-phlorizin hydrolase
Genes
HSA: 
3938(LCT)
PTR: 
459633(LCT)
PPS: 
GGO: 
PON: 
MCC: 
707761(LCT)
MMU: 
226413(Lct)
RNO: 
116569(Lct)
CFA: 
483898(LCT)
AML: 
FCA: 
101092452(LCT)
BTA: 
514332(LCT)
SSC: 
100625897
ECB: 
100055369
SHR: 
100933904(LCT)
OAA: 
100084941(LCT)
GGA: 
424294(LCT)
TGU: 
100229941(LCT)
XLA: 
100127248(lct.1)
XTR: 
100145766(lct.2)
DRE: 
796436
TRU: 
101066689
OLA: 
101164357 101171223
BFO: 
BRAFLDRAFT_105101(SUFU)
CIN: 
100186561
SPU: 
587972
AAG: 
AaeL_AAEL009246
CQU: 
CpipJ_CPIJ008529
NVE: 
NEMVE_v1g201226
VVI: 
100267558
 » show all
Taxonomy
Reference
1  [PMID:4752949]
  Authors
Leese HJ, Semenza G.
  Title
On the identity between the small intestinal enzymes phlorizin hydrolase and glycosylceramidase.
  Journal
J. Biol. Chem. 248 (1973) 8170-3.
  Organism
Homo sapiens [GN:hsa], Rattus norvegicus [GN:rno], hamster
Reference
2  [PMID:5082068]
  Authors
Lorenz-Meyer H, Blum AL, Haemmerli HP, Semenza G.
  Title
A second enzyme defect in acquired lactase deficiency: lack of small-intestinal phlorizin-hydrolase.
  Journal
Eur. J. Clin. Invest. 2 (1972) 326-31.
  Organism
Homo sapiens [GN:hsa]
Reference
3  [PMID:5774775]
  Authors
Malathi P, Crane RK.
  Title
Phlorizin hydrolase: a beta-glucosidase of hamster intestinal brush border membrane.
  Journal
Biochim. Biophys. Acta. 173 (1969) 245-56.
  Organism
hamster
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9033-10-7
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