KEGG   ENZYME: 3.2.1.68Help
Entry
EC 3.2.1.68                 Enzyme                                 

Name
isoamylase;
debranching enzyme;
glycogen alpha-1,6-glucanohydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
glycogen 6-alpha-D-glucanohydrolase
Reaction(IUBMB)
Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen, amylopectin and their beta-limit dextrins
Comment
Also readily hydrolyses amylopectin. Differs from EC 3.2.1.41 (pullulanase) and EC 3.2.1.142 (limit dextrinase) by its inability to hydrolyse pullulan, and by limited action on alpha-limit dextrins. Maltose is the smallest sugar it can release from an alpha-(1->6)-linkage.
History
EC 3.2.1.68 created 1972, modified 1976, modified 2000
Orthology
K01214  
isoamylase
Genes
TAD: 
ATH: 
AT2G39930(ISA1) AT4G09020(ISA3)
ALY: 
CRB: 
EUS: 
BRP: 
CIT: 
CIC: 
TCC: 
EGR: 
GMX: 
PVU: 
MTR: 
CAM: 
FVE: 
PPER: 
PMUM: 
MDM: 
PXB: 
CSV: 
CMO: 
RCU: 
POP: 
POPTR_0002s10110g(POPTRDRAFT_754756) POPTR_0006s07010g(POPTRDRAFT_560622) POPTR_0018s13240g(POPTRDRAFT_1107375)
VVI: 
SLY: 
SOT: 
BVG: 
OSA: 
DOSA: 
Os08t0520900-00(Os08g0520900) Os09t0469400-01(Os09g0469400)
OBR: 
BDI: 
SBI: 
SORBI_07g027200(SORBIDRAFT_07g027200)
ZMA: 
103649172 103654472 542095(iso3) 542318(isoamylase)
SITA: 
PDA: 
MUS: 
ATR: 
s00008p00166810(AMTR_s00008p00166810) s00025p00193210(AMTR_s00025p00193210)
SMO: 
PPP: 
CRE: 
VCN: 
OLU: 
OTA: 
BPG: 
MIS: 
MPP: 
CSL: 
CVR: 
CME: 
GSL: 
CCP: 
 » show all
Taxonomy
Reference
1  [PMID:5456995]
  Authors
Yokobayashi K, Misaki A, Harada T.
  Title
Purification and properties of Pseudomonas isoamylase.
  Journal
Biochim. Biophys. Acta. 212 (1970) 458-69.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9067-73-6

DBGET integrated database retrieval system