KEGG   ENZYME: 3.2.1.68Help
Entry
EC 3.2.1.68                 Enzyme                                 

Name
isoamylase;
debranching enzyme;
glycogen alpha-1,6-glucanohydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
BRITE hierarchy
Sysname
glycogen 6-alpha-D-glucanohydrolase
Reaction(IUBMB)
Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen, amylopectin and their beta-limit dextrins
Comment
Also readily hydrolyses amylopectin. Differs from EC 3.2.1.41 (pullulanase) and EC 3.2.1.142 (limit dextrinase) by its inability to hydrolyse pullulan, and by limited action on alpha-limit dextrins. Maltose is the smallest sugar it can release from an alpha-(1->6)-linkage.
History
EC 3.2.1.68 created 1972, modified 1976, modified 2000
Orthology
K01214  
isoamylase
Genes
CME: 
GKA: 
RHA: 
Taxonomy
Reference
1  [PMID:5456995]
  Authors
Yokobayashi K, Misaki A, Harada T.
  Title
Purification and properties of Pseudomonas isoamylase.
  Journal
Biochim. Biophys. Acta. 212 (1970) 458-69.
  Organism
Pseudomonas sp.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9067-73-6

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