| Entry |
|
|---|
| Name |
purine nucleosidase;
nucleosidase;
purine beta-ribosidase;
purine nucleoside hydrolase;
purine ribonucleosidase;
ribonucleoside hydrolase;
nucleoside hydrolase;
N-ribosyl purine ribohydrolase;
nucleosidase g;
N-D-ribosylpurine ribohydrolase;
inosine-adenosine-guanosine preferring nucleoside hydrolase;
purine-specific nucleoside N-ribohydrolase;
IAG-nucleoside hydrolase;
IAG-NH |
|---|
| Class |
Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds
 |
|---|
| Sysname |
purine-nucleoside ribohydrolase |
|---|
| Reaction(IUBMB) |
a purine nucleoside + H2O = D-ribose + a purine base [RN:R02341] |
|---|
| Reaction(KEGG) |
R02341 > R01245 R01677 R01770 R02143;
(other) R01273
 |
|---|
| Substrate |
purine nucleoside [CPD:C15586];
H2O [CPD:C00001] |
|---|
| Product |
D-ribose [CPD:C00121];
purine base [CPD:C15587] |
|---|
| Comment |
The enzyme from the bacterium Ochrobactrum anthropi specifically
catalyses the irreversible N-riboside hydrolysis of purine
nucleosides. Pyrimidine nucleosides, purine and pyrimidine
nucleotides, NAD+, NADP+ and nicotinaminde mononucleotide are not
substrates [6]. |
|---|
| Pathway |
PATH: ec00230 Purine metabolism
PATH: ec00760 Nicotinate and nicotinamide metabolism
PATH: ec01100 Metabolic pathways |
|---|
| Orthology |
KO: K01239 purine nucleosidase |
|---|
| Genes |
XLA: 379157
BFO: BRAFLDRAFT_115361
CIN: 100187045
AGA: AgaP_AGAP011729
AAG: AaeL_AAEL002176
CQU: CpipJ_CPIJ012557 CpipJ_CPIJ014047
NVI: 100114188 100114270
API: 100165637 100168510
ISC: IscW_ISCW011480 IscW_ISCW013084
CBR: CBG02610
HMG: 100197535
TAD: TRIADDRAFT_28473 TRIADDRAFT_28800
VVI: 100266594
CRE: CHLREDRAFT_171013 CHLREDRAFT_23981
OLU: OSTLU_28809
SPO: SPBC1683.06c
MBR: MONBRDRAFT_8168
DDI: DDB_0231227(iunH)
EHI: EHI_199960
EDI: EDI_192100
TET: TTHERM_00666430 TTHERM_00766410
PTM: GSPATT00018892001 GSPATT00019881001
TBR: Tb927.3.2960 Tb927.7.4570
TCR: 508153.640 510315.10
LMA: LmjF29.2800
LIF: LinJ29.3130
LBZ: LbrM29_V2.2850
TVA: TVAG_213720
ECA: ECA4423 ECA4424
PCT: PC1_0017 PC1_0018
PWA: Pecwa_0017 Pecwa_0018
PLU: plu2773 plu4289(iunH)
PAY: PAU_00466(iunH) PAU_01770(rihB)
SPE: Spro_4396
DDA: Dd703_1442 Dd703_1443
DDC: Dd586_2533 Dd586_2534
DZE: Dd1591_2617 Dd1591_2618
PMU: PM1767(iunH)
XCC: XCC3237
XCB: XC_0946
XCA: xccb100_0948(rlhA)
XCV: XCV3507(iunH)
XAC: XAC3390
XOO: XOO1152
XOM: XOO_1049
XOP: PXO_02361
SML: Smlt1222 Smlt3837
SMT: Smal_1071 Smal_3255
PAE: PA0143(nuh)
PAU: PA14_01760(nuh)
PAP: PSPA7_0220
PAG: PLES_01441(nuh)
PPU: PP_2460
PPF: Pput_3230
PPG: PputGB1_3486
PPW: PputW619_1962
PST: PSPTO_2373
PSB: Psyr_2157
PSP: PSPPH_2131 PSPPH_2471
PFL: PFL_2107
PFO: Pfl01_1925
PFS: PFLU4154
PEN: PSEEN1958
ACI: ACIAD2386(iunH)
PIN: Ping_1902
RSO: RSc2548
BUR: Bcep18194_B0386
BCN: Bcen_3104
BCH: Bcen2424_5263
BCM: Bcenmc03_5010
BCJ: BCAM2461
BAM: Bamb_4624
BAC: BamMC406_5150
BPY: Bphyt_4926
BGL: bglu_2g11590
VAP: Vapar_0832
DDE: Dde_0760
SCL: sce4961
MLO: mll3190 mlr2009
SME: SMc01105
SMD: Smed_0050 Smed_2802 Smed_3659 Smed_4609
ATU: Atu0374(iunH)
ATC: AGR_C_654
ARA: Arad_0265 Arad_0668(iunH1) Arad_2787(iunH2)
AVI: Avi_0448(iunH) Avi_2632(iunH)
RET: RHE_CH00366(iunH1) RHE_CH02485(iunH2)
REC: RHECIAT_CH0000405(iunH1) RHECIAT_CH0002588(iunH2)
RHECIAT_CH0003663(iunH3)
RLE: RL0383(iunH) RL2825 RL3882(iunH) pRL120178
RLT: Rleg2_0009 Rleg2_2125 Rleg2_3163
RLG: Rleg_0009 Rleg_2367
RHI: NGR_c00520 NGR_c29640
BME: BMEII0088
BMI: BMEA_B0008
BMF: BAB2_0005
BMB: BruAb2_0006(iunH)
BMC: BAbS19_II00050
BMS: BRA0006(iunH)
BMT: BSUIS_B0008
BOV: BOV_A0003(iunH)
BCS: BCAN_B0008
BMR: BMI_II6(iunH)
OAN: Oant_4446
CCS: CCNA_02005
CAK: Caul_2809
SIL: SPO2470(iunH)
SIT: TM1040_0934
RSP: RSP_2914
RSH: Rsph17029_1558
RSQ: Rsph17025_1107
RSK: RSKD131_1231
JAN: Jann_1804
RDE: RD1_3134
PDE: Pden_4001
DSH: Dshi_1158(rih)
GOX: GOX0947
GBE: GbCGDNIH1_1340
RCE: RC1_2455(rihA)
BAN: BA2400 BA2888 BA3606 BA5338
BAR: GBAA2400 GBAA2888 GBAA3606 GBAA5338
BAA: BA_0199 BA_2897 BA_3408 BA_4098
BAT: BAS2236 BAS2693 BAS4961
BAH: BAMEG_1024 BAMEG_1711 BAMEG_2197 BAMEG_5393
BAI: BAA_2460 BAA_2947 BAA_3635 BAA_5370
BCE: BC2331 BC2683 BC2889 BC3552 BC5134
BCA: BCE_2927 BCE_3566
BCZ: BCZK2156(iunH) BCZK2619(iunH) BCZK3260 BCZK4823
BCR: BCAH187_A2500 BCAH187_A2939 BCAH187_A3569 BCAH187_A5283
BCB: BCB4264_A2364 BCB4264_A2696 BCB4264_A2904 BCB4264_A3659
BCB4264_A5248
BCU: BCAH820_2415 BCAH820_2891 BCAH820_3559 BCAH820_5219
BCG: BCG9842_B1658 BCG9842_B2341 BCG9842_B2614 BCG9842_B2968
BCG9842_B5704
BCQ: BCQ_2325 BCQ_2726(iunH) BCQ_3345 BCQ_4939
BCX: BCA_2468 BCA_2968 BCA_3645 BCA_5245
BCY: Bcer98_1982 Bcer98_2226
BTK: BT9727_2170(iunH) BT9727_2642(iunH) BT9727_3310 BT9727_4813
BTL: BALH_2139 BALH_2595(iunH) BALH_3191(iunH) BALH_4626(iunH)
BWE: BcerKBAB4_2208 BcerKBAB4_2467 BcerKBAB4_2695 BcerKBAB4_3254
BcerKBAB4_4926
BLI: BL02627
BLD: BLi00740
BCL: ABC3205 ABC3550 ABC3998
BPU: BPUM_0649 BPUM_3604
SAB: SAB2155c
SAJ: SaurJH9_0228
SAH: SaurJH1_0234
SEP: SE0755
SER: SERP0642
SHA: SH1905
SSP: SSP1735
SCA: Sca_0677
LSP: Bsph_2870
BBE: BBR47_56890
PJD: Pjdr2_3298 Pjdr2_5891
SAG: SAG0528
SAN: gbs0574
SAK: SAK_0679
SUB: SUB0724
SDS: SDEG_0227
LPL: lp_0363 lp_2591 lp_2825
LPJ: JDM1_0325 JDM1_2081 JDM1_2267
LJO: LJ0799 LJ1069
LJF: FI9785_1142 FI9785_650
LAC: LBA0591(iunH) LBA0865
LSA: LSA0252(iunH1) LSA0533(iunH2) LSA0830(iunH3)
LSL: LSL_0819(uRH) LSL_1528(uRH)
LDB: Ldb0532 Ldb0815
LBU: LBUL_0473 LBUL_0745
LBR: LVIS_0403 LVIS_1816 LVIS_2069
LCB: LCABL_03800
LGA: LGAS_0558 LGAS_0870
LRE: Lreu_1658
LRF: LAR_0096 LAR_1547
LHE: lhv_0614 lhv_0918
LFE: LAF_1704
LRL: LC705_00392(rihC)
PPE: PEPE_0161 PEPE_1424
EFA: EF1921 EF2587
OOE: OEOE_0154 OEOE_1461 OEOE_1673
LME: LEUM_0296 LEUM_0759 LEUM_0797 LEUM_0798
LCI: LCK_00248 LCK_00288 LCK_00694
CPF: CPF_2260
CPR: CPR_1971
CTC: CTC00577
CDF: CD1682(iunH)
CDC: CD196_1605(iunH)
CDL: CDR20291_1580(iunH)
CBO: CBO2621
CBA: CLB_2563
CBH: CLC_2494
CBY: CLM_2984
CBL: CLK_2007
CBK: CLL_A3021
CBB: CLD_1944
CBI: CLJ_B2851
CBT: CLH_2769
CBF: CLI_2685
CPY: Cphy_1819
DSY: DSY3527
DHD: Dhaf_1878
FMA: FMG_1442
APR: Apre_0309
MTU: Rv3393(iunH)
MTC: MT3500
MTF: TBFG_13427
MTB: TBMG_03443(TBMG_03443.1)
MBO: Mb3425(iunH)
MBB: BCG_3462(iunH)
MBT: JTY_3462(iunH)
MAV: MAV_4342
MMI: MMAR_1152(iunH)
CGL: NCgl1309(cgl1364) NCgl2736(cgl2835)
CGB: cg1543(iunH3) cg3137(iunH1)
CGT: cgR_1429 cgR_2720
CEF: CE1482
CDI: DIP1753(iunH)
CJK: jk0233(iunH1) jk2078(iunH2)
CUR: cur_1768 cur_2003
CAR: cauri_1522(iunH)
CKP: ckrop_1727(iunH)
RHA: RHA1_ro00276 RHA1_ro06433 RHA1_ro07236(iunH)
RER: RER_30920
ROP: ROP_04020 ROP_70200
GBR: Gbro_2431
CMI: CMM_0982(iunH)
CMS: CMS_2856
ART: Arth_0081 Arth_1012
AAU: AAur_1125 AAur_3646
ACH: Achl_0185 Achl_1642
RSA: RSal33209_3202
MLU: Mlut_06650
BCV: Bcav_0275
PAC: PPA2076
TFU: Tfu_2581
KRA: Krad_1901
SEN: SACE_0339 SACE_3233
AMI: Amir_2621
SAQ: Sare_1207
BLO: BL1770
BLJ: BLD_1062(urhA2)
BLN: Blon_0638 Blon_2007
BAD: BAD_1486(iunH2)
BLA: BLA_0639(iunH) BLA_0696(iunH)
BLC: Balac_1438 Balac_1503
BLT: Balat_1438 Balat_1503
CCU: Ccur_04420
APV: Apar_1192
ACA: ACP_2653
PDI: BDI_1655
RBA: RB417(iunH)
CYA: CYA_1903
CYB: CYB_0491
CYT: cce_3186
ANA: alr3202 alr3285
NPU: Npun_R6607
AVA: Ava_3071 Ava_3900 Ava_4938
TER: Tery_3465
RRS: RoseRS_2633
RCA: Rcas_3042
CAU: Caur_0484
CAG: Cagg_3224
CHL: Chy400_0519
TRO: trd_1029
DRA: DR_0403
DGE: Dgeo_1652
DDR: Deide_15030
TTR: Tter_2311
HMA: rrnAC0226(iunH2)
HWA: HQ2498A(iunH)
SSO: SSO0505(iunH-1) SSO2243(iunH-2)
STO: ST0803 ST2115
SAI: Saci_0047 Saci_1187(iunH)
SIS: LS215_0057 LS215_1728
SIA: M1425_0057 M1425_1602
SIM: M1627_0057 M1627_1717
SID: M164_0057 M164_1648
SIY: YG5714_0057 YG5714_1692
SIN: YN1551_0057 YN1551_1212
MSE: Msed_0768 Msed_1006
 |
|---|
| Structures |
PDB: 1EZR 1HOZ 1HP0 1KIC 1KIE 1MAS 1R4F 2C40 2FF1 2FF2
2MAS 3B9G 3EPW 3EPX 3FZ0 |
|---|
Reference
Authors
Title
Journal
Organism
|
1 [PMID:12999785]
HEPPEL LA, HILMOE RJ.
[Phosphorolysis and hydrolysis of purine ribosides by enzymes from
yeast.]
J. Biol. Chem. 198 (1952) 683-94.
Saccharomyces cerevisiae [GN:sce] |
|---|
Reference
Authors
Title
Journal
|
2
Kalckar, H.M.
Biosynthetic aspects of nucleosides and nucleic acids.
Pubbl. Staz. Zool. (Napoli) (1951) 87-103. |
|---|
Reference
Authors
Title
Journal
Organism
|
3 [PMID:13416219]
TAKAGI Y, HORECKER BL.
Purification and properties of a bacterial riboside hydrolase.
J. Biol. Chem. 225 (1957) 77-86.
Lactobacillus delbrueckii, Lactobacillus pentmus |
|---|
Reference
Authors
Title
Journal
Organism
|
4 [PMID:14363106]
TARR HL.
Fish muscle riboside hydrolases.
Biochem. J. 59 (1955) 386-91.
Ophiodon elonatus, Sebastodes sp. |
|---|
Reference
Authors
Title
Journal
Organism
|
5 [PMID:8702965]
Parkin DW.
Purine-specific nucleoside N-ribohydrolase from Trypanosoma brucei
brucei. Purification, specificity, and kinetic mechanism.
J. Biol. Chem. 271 (1996) 21713-9.
Trypanosoma brucei [GN:tbr] |
|---|
Reference
Authors
Title
Journal
Organism
|
6 [PMID:11282633]
Ogawa J, Takeda S, Xie SX, Hatanaka H, Ashikari T, Amachi T, Shimizu
S.
Purification, characterization, and gene cloning of purine
nucleosidase from Ochrobactrum anthropi.
Appl. Environ. Microbiol. 67 (2001) 1783-7.
Ochrobactrum anthropi [GN:oan] |
|---|
Reference
Authors
Title
Journal
Organism
|
7 [PMID:11854281]
Versees W, Decanniere K, Van Holsbeke E, Devroede N, Steyaert J.
Enzyme-substrate interactions in the purine-specific nucleoside
hydrolase from Trypanosoma vivax.
J. Biol. Chem. 277 (2002) 15938-46.
Trypanosoma vivax |
|---|
Reference
Authors
Title
Journal
Organism
|
8 [PMID:15909995]
Mazumder-Shivakumar D, Bruice TC.
Computational study of IAG-nucleoside hydrolase: determination of
the preferred ground state conformation and the role of active site
residues.
Biochemistry. 44 (2005) 7805-17.
Trypanosoma vivax |
|---|
| Other DBs |
ExplorEnz - The Enzyme Database: 3.2.2.1
IUBMB Enzyme Nomenclature: 3.2.2.1
ExPASy - ENZYME nomenclature database: 3.2.2.1
BRENDA, the Enzyme Database: 3.2.2.1
CAS: 9025-44-9 |
|---|
