KEGG   ENZYME: 3.2.2.1Help
Entry
EC 3.2.2.1                  Enzyme                                 
Name
purine nucleosidase;
nucleosidase (misleading);
purine beta-ribosidase;
purine nucleoside hydrolase;
purine ribonucleosidase;
ribonucleoside hydrolase (misleading);
nucleoside hydrolase (misleading);
N-ribosyl purine ribohydrolase;
nucleosidase g;
N-D-ribosylpurine ribohydrolase;
inosine-adenosine-guanosine preferring nucleoside hydrolase;
purine-specific nucleoside N-ribohydrolase;
IAG-nucleoside hydrolase;
IAG-NH
Class
Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds
BRITE hierarchy
Sysname
purine-nucleoside ribohydrolase
Reaction(IUBMB)
a purine nucleoside + H2O = D-ribose + a purine base [RN:R02341]
Reaction(KEGG)
Substrate
purine nucleoside [CPD:C15586];
H2O [CPD:C00001]
Product
D-ribose [CPD:C00121];
purine base [CPD:C15587]
Comment
The enzyme from the bacterium Ochrobactrum anthropi specifically catalyses the irreversible N-riboside hydrolysis of purine nucleosides. Pyrimidine nucleosides, purine and pyrimidine nucleotides, NAD+, NADP+ and nicotinaminde mononucleotide are not substrates [6].
Pathway
Purine metabolism
Nicotinate and nicotinamide metabolism
Metabolic pathways
Orthology
K01239  
purine nucleosidase
Genes
XLA: 
OLU: 
SPO: 
DDI: 
EHI: 
EHI_199960(42.t00042)
EDI: 
TET: 
TBR: 
Tb927.3.2960(Tb03.27C5.420) Tb927.7.4570(Tb07.26A24.720)
TCR: 
LIF: 
TVA: 
TVAG_213720
ECA: 
ECA4423 ECA4424
PCT: 
PC1_0017 PC1_0018
PWA: 
Pecwa_0017 Pecwa_0018
PLU: 
plu2773 plu4289(iunH)
PAY: 
PAU_00466(iunH) PAU_01770(rihB)
SPE: 
Spro_4396
DDA: 
Dd703_1442 Dd703_1443
DDC: 
Dd586_2533 Dd586_2534
DZE: 
Dd1591_2617 Dd1591_2618
XBO: 
XBJ1_3558(rihB)
XNE: 
XNC1_3617(rihB)
RAH: 
Rahaq_1087 Rahaq_4714
PSI: 
S70_07980
PMU: 
PM1767(iunH)
XCC: 
XCC3237
XCB: 
XC_0946
XCA: 
xccb100_0948(rlhA)
XCV: 
XCV3507(iunH)
XAC: 
XAC3390
XOO: 
XOO1152
XOM: 
XOO_1049
XOP: 
PXO_02361
SML: 
Smlt1222 Smlt3837
SMT: 
Smal_3255
PSU: 
Psesu_0694
FAU: 
Fraau_2787
PAE: 
PA0143(nuh)
PAU: 
PA14_01760(nuh)
PAP: 
PSPA7_0220
PAG: 
PLES_01441(nuh)
PAF: 
PAM18_0144(nuh)
PNC: 
NCGM2_0148(nuh)
PDK: 
PADK2_00725
PPU: 
PP_2460
PPF: 
Pput_3230
PPG: 
PputGB1_3486
PPW: 
PputW619_1962
PST: 
PSPTO_2373
PSB: 
Psyr_2157
PSP: 
PSPPH_2131 PSPPH_2471
PFL: 
PFL_2107(rihA)
PFO: 
Pfl01_1925
PFS: 
PFLU4154
PEN: 
PSEEN1958
ACI: 
ACIAD2386(iunH)
PIN: 
Ping_1902
PSE: 
NH8B_1796
RSO: 
RSc2548
RSL: 
RPSI07_0919
BUR: 
Bcep18194_B0386
BCN: 
Bcen_3104
BCH: 
Bcen2424_5263
BCM: 
Bcenmc03_5010
BCJ: 
BCAM2461
BAM: 
Bamb_4624
BAC: 
BamMC406_5150
BGL: 
bglu_2g11590
BGD: 
bgla_2g15030
VAP: 
Vapar_0832
VPE: 
Varpa_0856
CFU: 
CFU_2907
DDE: 
Dde_0760
MLO: 
mll3190 mlr2009
MCI: 
Mesci_1748
MOP: 
Mesop_1807
MAM: 
Mesau_01814
SME: 
SMc01105
SMK: 
Sinme_0048
SMQ: 
SinmeB_0048
SMX: 
SM11_chr0049(iunH)
SMI: 
BN406_00048
SMEG: 
C770_GR4Chr0424
SMD: 
Smed_0050
RHI: 
NGR_c00520
SFH: 
SFHH103_00051
SFD: 
USDA257_c00530(rihA)
ATU: 
Atu0374(iunH)
ARA: 
Arad_0668(iunH1)
AVI: 
Avi_0448(iunH)
AGR: 
AGROH133_03580(iunH)
RET: 
RHE_CH00366(iunH1) RHE_CH02485(iunH2)
REC: 
RHECIAT_CH0000405(iunH1)
RLE: 
RL0383(iunH) RL2825 RL3882(iunH) pRL120178
RLT: 
Rleg2_0009
RLG: 
Rleg_0009
BME: 
BMEII0088
BMI: 
BMEA_B0008
BMF: 
BAB2_0005
BMB: 
BruAb2_0006(iunH)
BMC: 
BAbS19_II00050
BMS: 
BRA0006(iunH)
BSI: 
BS1330_II0006(iunH)
BMT: 
BSUIS_B0008
BSV: 
BSVBI22_B0006(iunH)
BOV: 
BOV_A0003(iunH)
BCS: 
BCAN_B0008
BSK: 
BCA52141_II1236
BMR: 
BMI_II6(iunH)
BPP: 
BPI_II6(iunH)
OAN: 
Oant_4446
PHL: 
KKY_2267
CCR: 
CC_1928
CCS: 
CCNA_02005
CAK: 
Caul_2809
CSE: 
Cseg_2215
BSB: 
Bresu_3199
SIL: 
SPO2470(iunH)
SIT: 
TM1040_0934
RSP: 
RSP_2914
RSH: 
Rsph17029_1558
RSQ: 
Rsph17025_1107
RSK: 
RSKD131_1231
JAN: 
Jann_1804
RDE: 
RD1_3134
PDE: 
Pden_4001
DSH: 
Dshi_1158(rih)
KVU: 
EIO_1176 EIO_2348
GOX: 
GOX0947
GBE: 
GbCGDNIH1_1340
RCE: 
RC1_2455(rihA)
BLI: 
BL02627
BLD: 
BLi00740
BAN: 
BA_2400 BA_2888 BA_3606 BA_5338
BAR: 
GBAA_2400 GBAA_2888 GBAA_3606 GBAA_5338
BAT: 
BAS2236 BAS2693 BAS4961
BAH: 
BAMEG_1024 BAMEG_1711 BAMEG_2197 BAMEG_5393
BAI: 
BAA_2460 BAA_2947 BAA_3635 BAA_5370
BAL: 
BACI_c23470 BACI_c34950 BACI_c51160
BCE: 
BC2331 BC2683 BC2889 BC3552 BC5134
BCA: 
BCE_2927 BCE_3566
BCZ: 
BCZK2156(iunH) BCZK2619(iunH) BCZK3260 BCZK4823
BCR: 
BCAH187_A2500 BCAH187_A2939 BCAH187_A3569 BCAH187_A5283
BCB: 
BCB4264_A2364 BCB4264_A2904 BCB4264_A3659 BCB4264_A5248
BCU: 
BCAH820_2415 BCAH820_2891 BCAH820_3559 BCAH820_5219
BCG: 
BCG9842_B1658 BCG9842_B2341 BCG9842_B2968 BCG9842_B5704
BCQ: 
BCQ_2325 BCQ_2726(iunH) BCQ_3345 BCQ_4939
BCX: 
BCA_2468 BCA_2469 BCA_2968 BCA_3645 BCA_5245
BCY: 
Bcer98_1982 Bcer98_2226
BTK: 
BT9727_2170(iunH) BT9727_2642(iunH) BT9727_3310 BT9727_4813
BTL: 
BALH_2139 BALH_2595(iunH) BALH_3191(iunH) BALH_4626(iunH)
BTB: 
BMB171_C2098 BMB171_C2593 BMB171_C3226 BMB171_C4706
BWE: 
BcerKBAB4_2208 BcerKBAB4_2695 BcerKBAB4_3254 BcerKBAB4_4926
BCL: 
ABC3205 ABC3550 ABC3998
BPU: 
BPUM_0649 BPUM_3604
BPF: 
BpOF4_09550 BpOF4_19070
BMQ: 
BMQ_0541
BMD: 
BMD_0544
SAU: 
SA2078
SAV: 
SAV2283
SAW: 
SAHV_2267
SAH: 
SaurJH1_0234 SaurJH1_2350
SAJ: 
SaurJH9_0228 SaurJH9_2308
SAM: 
MW2201
SAS: 
SAS2173
SAR: 
SAR2367
SAC: 
SACOL2276
SAX: 
USA300HOU_2265
SAA: 
SAUSA300_2234
SAO: 
SAOUHSC_02553
SAE: 
NWMN_2183
SAD: 
SAAV_2346
SUU: 
M013TW_2241
SUV: 
SAVC_10280
SUH: 
SAMSHR1132_21090
SUE: 
SAOV_2322c
SUJ: 
SAA6159_02188
SUK: 
SAA6008_02320
SUC: 
ECTR2_2144
SUT: 
SAT0131_02461(isdF)
SUQ: 
HMPREF0772_10915
SUZ: 
MS7_2302
SUD: 
ST398NM01_2334
SUX: 
SAEMRSA15_21810
SUW: 
SATW20_24150
SUG: 
SAPIG2334
SAB: 
SAB2155c
SUY: 
SA2981_2221
SAUB: 
C248_2321
SEP: 
SE0755 SE1857
SER: 
SERP0642 SERP1865
SHA: 
SH0769 SH1905
SSP: 
SSP0210 SSP1735
SCA: 
Sca_1776
SLG: 
SLGD_00742
SLN: 
SLUG_07400
SSD: 
SPSINT_2246
SDT: 
SPSE_0222
SWA: 
A284_03155
LSP: 
Bsph_2870
BBE: 
BBR47_56890
PJD: 
Pjdr2_5891
PPY: 
PPE_04610
PPM: 
PPSC2_c5116
SAG: 
SAG0528
SAN: 
gbs0574
SAK: 
SAK_0679
SDS: 
SDEG_0227
LPL: 
lp_2591 lp_2825
LPJ: 
JDM1_2081 JDM1_2267
LPS: 
LPST_C2132 LPST_C2319
LJO: 
LJ0799 LJ1069
LJF: 
FI9785_1142
LAC: 
LBA0591(iunH) LBA0865
LSA: 
LSA0252(iunH1) LSA0533(iunH2) LSA0830(iunH3)
LSL: 
LSL_0819(uRH) LSL_1528(uRH)
LDB: 
Ldb0532 Ldb0815
LBU: 
LBUL_0473 LBUL_0745
LDE: 
LDBND_0742
LBR: 
LVIS_0403 LVIS_1816 LVIS_2069
LGA: 
LGAS_0558 LGAS_0870
LRE: 
Lreu_1658
LRF: 
LAR_1547
LRU: 
HMPREF0538_20831(urh)
LHE: 
lhv_0918
LFE: 
LAF_1704
LCR: 
LCRIS_00885(iunH2)
LBH: 
Lbuc_0486
PPE: 
PEPE_0161 PEPE_1424
EFA: 
EF1921 EF2587
MPS: 
MPTP_1126
THL: 
TEH_18810 TEH_18890
OOE: 
OEOE_0154 OEOE_1461 OEOE_1673
LME: 
LEUM_0296 LEUM_0759 LEUM_0798
LCI: 
LCK_00288 LCK_00694
LKI: 
LKI_03320 LKI_05160 LKI_09780
LGS: 
LEGAS_0022(rihA1) LEGAS_0456 LEGAS_1534(rihA2)
LEC: 
LGMK_02630 LGMK_07265 LGMK_09020
AUR: 
HMPREF9243_0631 HMPREF9243_1031 HMPREF9243_1722
WKO: 
WKK_03050 WKK_04140
CPF: 
CPF_2260
CPR: 
CPR_1971
CBO: 
CBO2621
CBK: 
CLL_A3021
CBT: 
CLH_2769
CDF: 
CD630_16820(iunH)
CDC: 
CD196_1605(iunH)
CDL: 
CDR20291_1580(iunH)
DSY: 
DSY3527
FMA: 
FMG_1442
ELM: 
ELI_2205
TMR: 
Tmar_0243
MTU: 
Rv3393(iunH)
MTV: 
RVBD_3393
MTC: 
MT3500
MRA: 
MRA_3432(iunH)
MTF: 
TBFG_13427
MTB: 
TBMG_03443
MTK: 
TBSG_03465
MTZ: 
TBXG_003418
MTG: 
MRGA327_20945
MTI: 
MRGA423_21380
MTO: 
MTCTRI2_3464(iunH)
MTD: 
UDA_3393(iunH)
MBO: 
Mb3425(iunH)
MBB: 
BCG_3462(iunH)
MBT: 
JTY_3462(iunH)
MBM: 
BCGMEX_3460(iunH)
MAF: 
MAF_34060(iunH)
MCE: 
MCAN_34161(iunH)
MCV: 
BN43_70045(iunH)
MCX: 
BN42_50050(iunH)
MCZ: 
BN45_70035(iunH)
MAV: 
MAV_4342
MMI: 
MMAR_1152(iunH)
MLI: 
MULP_01294(iunH)
CGL: 
NCgl1309(Cgl1364) NCgl2736(Cgl2835)
CGB: 
cg1543(iunH3) cg3137(iunH1)
CGT: 
cgR_1429 cgR_2720
CEF: 
CE1482
CDI: 
DIP1753(iunH)
CDP: 
CD241_1688(iunH)
CDH: 
CDB402_1642(iunH)
CDT: 
CDHC01_1691(iunH)
CDE: 
CDHC02_1625(iunH)
CDR: 
CDHC03_1654(iunH)
CDA: 
CDHC04_1650(iunH)
CDZ: 
CD31A_1759(iunH)
CDB: 
CDBH8_1726(iunH)
CDS: 
CDC7B_1740(iunH)
CDD: 
CDCE8392_1651(iunH)
CDW: 
CDPW8_1748(iunH)
CDV: 
CDVA01_1616(iunH)
CJK: 
jk0233(iunH1) jk2078(iunH2)
CUR: 
cur_2003
CKP: 
ckrop_1727(iunH)
CPU: 
cpfrc_01774(iunH1)
RHA: 
RHA1_ro00276 RHA1_ro06433 RHA1_ro07236(iunH)
CMI: 
CMM_0982(iunH)
CMS: 
CMS_2856
ART: 
Arth_0081 Arth_1012
AAU: 
AAur_1125 AAur_3646
ACH: 
Achl_0185
AAI: 
AARI_00440(iunH)
ARR: 
ARUE_c37820
RSA: 
RSal33209_3202
ICA: 
Intca_2825
PAC: 
PPA2076
NCA: 
Noca_0542
TFU: 
Tfu_2581
NDA: 
Ndas_4382
KRA: 
Krad_1901
SEN: 
SACE_0339 SACE_3233
BLN: 
Blon_0638
BAD: 
BAD_1486(iunH2)
BLA: 
BLA_0639(iunH)
BLC: 
Balac_1438
BLT: 
Balat_1438
GVA: 
HMPREF0424_0319 HMPREF0424_0577 HMPREF0424_1044
RXY: 
Rxyl_1896
CCU: 
Ccur_04420
SUS: 
Acid_6466
SLI: 
Slin_3262
RBA: 
RB417(iunH)
CYA: 
CYA_1903
CYB: 
CYB_0491
CYT: 
cce_3186
ANA: 
alr3202 alr3285
NPU: 
Npun_R6607
AVA: 
Ava_3071 Ava_3900 Ava_4938
TER: 
Tery_3465
RRS: 
RoseRS_2633
RCA: 
Rcas_2486 Rcas_3042
CAU: 
Caur_0484
CAG: 
Cagg_3224
CHL: 
Chy400_0519
DRA: 
DR_0403
DGE: 
Dgeo_1652
DDR: 
Deide_15030
DGO: 
DGo_CA1172(iunH)
OPR: 
Ocepr_0043
NDE: 
NIDE0314(rih)
HMA: 
rrnAC0226(iunH2)
HWA: 
HQ2498A(iunH)
HJE: 
HacjB3_13235
HBO: 
Hbor_34570
SSO: 
SSO0505(iunH-1) SSO2243(iunH-2)
STO: 
ST0803 ST2115
SAI: 
Saci_0047 Saci_1187(iunH)
SIS: 
LS215_0057 LS215_1728
SIA: 
M1425_0057 M1425_1602
SIM: 
M1627_0057 M1627_1717
SID: 
M164_0057 M164_1648
SIY: 
YG5714_0057 YG5714_1692
SIN: 
YN1551_0057 YN1551_1212
SII: 
LD85_0057 LD85_1857
MSE: 
Msed_0768 Msed_1006
 » show all
Taxonomy
Reference
1  [PMID:12999785]
  Authors
HEPPEL LA, HILMOE RJ.
  Title
[Phosphorolysis and hydrolysis of purine ribosides by enzymes from yeast.]
  Journal
J. Biol. Chem. 198 (1952) 683-94.
  Organism
Saccharomyces cerevisiae [GN:sce]
Reference
2
  Authors
Kalckar, H.M.
  Title
Biosynthetic aspects of nucleosides and nucleic acids.
  Journal
Pubbl. Staz. Zool. (Napoli) 23 (1951) 87-103.
Reference
3  [PMID:13416219]
  Authors
TAKAGI Y, HORECKER BL.
  Title
Purification and properties of a bacterial riboside hydrolase.
  Journal
J. Biol. Chem. 225 (1957) 77-86.
  Organism
Lactobacillus delbrueckii, Lactobacillus pentmus
Reference
4  [PMID:14363106]
  Authors
TARR HL.
  Title
Fish muscle riboside hydrolases.
  Journal
Biochem. J. 59 (1955) 386-91.
  Organism
Ophiodon elonatus, Sebastodes sp.
Reference
5  [PMID:8702965]
  Authors
Parkin DW.
  Title
Purine-specific nucleoside N-ribohydrolase from Trypanosoma brucei brucei. Purification, specificity, and kinetic mechanism.
  Journal
J. Biol. Chem. 271 (1996) 21713-9.
  Organism
Trypanosoma brucei [GN:tbr]
Reference
6  [PMID:11282633]
  Authors
Ogawa J, Takeda S, Xie SX, Hatanaka H, Ashikari T, Amachi T, Shimizu S.
  Title
Purification, characterization, and gene cloning of purine nucleosidase from Ochrobactrum anthropi.
  Journal
Appl. Environ. Microbiol. 67 (2001) 1783-7.
  Organism
Ochrobactrum anthropi [GN:oan]
Reference
7  [PMID:11854281]
  Authors
Versees W, Decanniere K, Van Holsbeke E, Devroede N, Steyaert J.
  Title
Enzyme-substrate interactions in the purine-specific nucleoside hydrolase from Trypanosoma vivax.
  Journal
J. Biol. Chem. 277 (2002) 15938-46.
  Organism
Trypanosoma vivax
Reference
8  [PMID:15909995]
  Authors
Mazumder-Shivakumar D, Bruice TC.
  Title
Computational study of IAG-nucleoside hydrolase: determination of the preferred ground state conformation and the role of active site residues.
  Journal
Biochemistry. 44 (2005) 7805-17.
  Organism
Trypanosoma vivax
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9025-44-9
DBGET integrated database retrieval system