KEGG   ENZYME: 3.4.17.21Help
Entry
EC 3.4.17.21                Enzyme                                 

Name
glutamate carboxypeptidase II;
N-acetylated-gamma-linked-acidic dipeptidase (NAALADase);
folate hydrolase;
prostate-specific membrane antigen;
pteroylpoly-gamma-glutamate carboxypeptidase;
microsomal gamma-glutamyl carboxypeptidase;
pteroylpolyglutamate hydrolase;
folylpolyglutamate hydrolase;
pteroylpoly-gamma-glutamate hydrolase;
pteroylpolygammaglutamyl hydrolase;
pteroylpolyglutamate hydrolase;
pteroylpolyglutamic acid hydrolase;
PSM antigen;
acetylaspartylglutamate dipeptidase;
NAALA dipeptidase;
rat NAAG peptidase;
mGCP;
membrane glutamate carboxypeptidase;
N-acetylated-alpha-linked-amino dipeptidase;
prostrate-specific membrane antigen;
N-Acetylated alpha-linked acidic dipeptidase;
PSMA
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metallocarboxypeptidases
BRITE hierarchy
Reaction(IUBMB)
Release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates [RN:R10687]
Reaction(KEGG)
R10687;
(other) R10688
Show
Comment
A metallo-carboxypeptidase that is predominantly expressed as a membrane-bound enzyme of 94-100 kDa , but also exists in a soluble form. Hydrolyses alpha-peptide bonds in Ac-Asp-Glu, Asp-Glu, and Glu-Glu, but also gamma-glutamyl bonds in gamma-Glu-Glu, and folylpoly-gamma-glutamates. With folylpoly-gamma-glutamates, shows processive carboxypeptidase activity to produce pteroylmonoglutamate [4]. Does not hydrolyse Ac-beta-Asp-Glu. Known inhibitors: quisqualic acid, Ac-beta-Asp-Glu, and 2-phosphonomethyl-pentanedioate. In peptidase family M28 of Vibrio leucyl aminopeptidase. The release of C-terminal glutamate from folylpoly-gamma-glutamates is also catalysed by EC 3.4.17.11 (glutamate carboxypeptidase) and EC 3.4.19.9 (gamma-Glu-X carboxypeptidase).
History
EC 3.4.17.21 created 1997, modified 2000 (EC 3.4.13.8 created 1972 and EC 3.4.19.8 created 1992, incorporated 2000)
Pathway
Alanine, aspartate and glutamate metabolism
Metabolic pathways
Orthology
K01301  
N-acetylated-alpha-linked acidic dipeptidase
K14592  
glutamate carboxypeptidase II (folate hydrolase 1)
Genes
HSA: 
10003(NAALAD2) 10004(NAALADL1) 2346(FOLH1)
PTR: 
451185(FOLH1) 451309(NAALADL1) 451475 451478(NAALAD2)
PPS: 
GGO: 
PON: 
100445995(NAALADL1) 100453848(FOLH1) 100460916(NAALAD2)
NLE: 
100579763(NAALADL1) 100580501 100582294(NAALAD2) 100589099(FOLH1B)
MCC: 
694180(NAALAD2) 707714(FOLH1) 717018(NAALADL1)
MCF: 
CJC: 
100396945(FOLH1B) 100406630(NAALAD2) 100413235(NAALADL1)
MMU: 
381204(Naaladl1) 53320(Folh1) 72560(Naalad2)
RNO: 
300384(Naalad2) 83568(Naaladl1) 85309(Folh1)
CGE: 
NGI: 
103737918(Naalad2) 103738766(Naaladl1) 103750517(Folh1)
HGL: 
101702083(Naaladl1) 101722660(Naalad2) 101725016(Folh1)
OCU: 
100339446(NAALAD2) 100358060(FOLH1B) 103346158(NAALADL1)
TUP: 
102475075(NAALADL1) 102487205(FOLH1B) 102488479(NAALAD2)
CFA: 
476775(FOLH1) 483751(NAALADL1) 485139(NAALAD2)
AML: 
100469236(NAALAD2) 100470423(NAALADL1) 100472805
UMR: 
103660690(NAALAD2) 103675371(FOLH1B) 103679566(NAALADL1)
FCA: 
101082777(NAALADL1) 101089389(NAALAD2) 101089641(FOLH1B)
PTG: 
102953631(NAALADL1) 102957765(FOLH1B) 102958523(NAALAD2)
BTA: 
505865(FOLH1) 506692(NAALADL1) 536439(NAALAD2)
BOM: 
102265480(NAALAD2) 102272952(FOLH1B) 102286181(NAALADL1)
PHD: 
102315808(NAALADL1) 102320530(NAALAD2) 102323016(FOLH1B)
CHX: 
102177776(NAALAD2) 102186842(NAALADL1) 102190665(FOLH1B)
OAS: 
101105198(NAALADL1) 101120451(NAALAD2) 101121723
SSC: 
100515250(NAALADL1) 102164324(NAALAD2) 397677(FOLH1)
CFR: 
102510657(FOLH1B) 102519706(NAALADL1) 102520257(NAALAD2)
BACU: 
103001153 103002601(NAALAD2) 103012348(FOLH1B) 103019730(NAALADL1)
LVE: 
103072597(NAALADL1) 103089873 103090789(NAALAD2)
ECB: 
100050187(NAALAD2) 100056368(NAALADL1) 100060132(FOLH1B)
MYB: 
102246530(NAALADL1) 102258767 102262061(NAALAD2)
MYD: 
102761189(FOLH1B) 102761368(NAALAD2) 102774994(NAALADL1)
PALE: 
102893898(NAALADL1) 102895068(FOLH1B) 102895568(NAALAD2)
MDO: 
100009990 100013563(NAALAD2) 100030670(NAALADL1)
SHR: 
100913549(NAALADL1) 100918727 100927408(NAALAD2)
OAA: 
GGA: 
419010(NAALAD2) 419011(FOLH1)
MGP: 
APLA: 
TGU: 
FAB: 
101814982(FOLH1) 101815312(NAALAD2)
PHI: 
102105283(FOLH1B) 102106596(NAALAD2)
FPG: 
FCH: 
102054726(NAALAD2) 102058819(FOLH1B)
CLV: 
102094196(NAALAD2) 102095234(FOLH1B)
ASN: 
102381492 102387337(NAALAD2) 102387587(FOLH1B)
AMJ: 
PSS: 
102454431(NAALADL1) 102455710(FOLH1B) 102456275(NAALAD2)
CMY: 
102933727(NAALAD2) 102934118(FOLH1B) 102942487(NAALADL1)
ACS: 
100551729 100551830(naaladl1) 100564848(naalad2)
PBI: 
XLA: 
100037180 734632(naalad2)
XTR: 
100485577(folh1) 100487005 594947(naalad2)
DRE: 
100000223(naaladl1) 393247(naalad2)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
CMK: 
BFO: 
CIN: 
SPU: 
PHU: 
ISC: 
CBR: 
LOA: 
LGI: 
SMM: 
NVE: 
HMG: 
TAD: 
AQU: 
ATH: 
ALY: 
CRB: 
EUS: 
BRP: 
CIT: 
CIC: 
TCC: 
GMX: 
PVU: 
CAM: 
FVE: 
PPER: 
PMUM: 
MDM: 
PXB: 
CSV: 
CMO: 
RCU: 
POP: 
POPTR_0006s15820g(POPTRDRAFT_1080900) POPTR_0006s21270g POPTR_0016s06430g(POPTRDRAFT_905420)
VVI: 
SLY: 
SOT: 
OSA: 
DOSA: 
Os01t0740500-00(Os01g0740500) Os01t0740650-00(Os01g0740650) Os01t0743300-01(Os01g0743300) Os03t0790600-01(Os03g0790600)
OBR: 
BDI: 
SBI: 
SORBI_01g005710(SORBIDRAFT_01g005710) SORBI_03g034150(SORBIDRAFT_03g034150) SORBI_03g034260(SORBIDRAFT_03g034260)
ZMA: 
SITA: 
PDA: 
MUS: 
ATR: 
s00004p00142600(AMTR_s00004p00142600) s00056p00161720(AMTR_s00056p00161720) s00056p00162910(AMTR_s00056p00162910)
SMO: 
PPP: 
CME: 
ERC: 
KLA: 
LTH: 
VPO: 
ZRO: 
NCS: 
NCAS_0J02130(NCAS0J02130)
NDI: 
NDAI_0J02840(NDAI0J02840)
TPF: 
TPHA_0C00760(TPHA0C00760) TPHA_0K00560(TPHA0K00560)
TBL: 
TBLA_0A04020(TBLA0A04020) TBLA_0B06200(TBLA0B06200)
TDL: 
TDEL_0D00700(TDEL0D00700)
KAF: 
KAFR_0E04260(KAFR0E04260)
PPA: 
DHA: 
PIC: 
PGU: 
SPAA: 
LEL: 
CAL: 
COT: 
CDU: 
CTEN: 
YLI: 
CLU: 
SMP: 
PAN: 
TTT: 
MTM: 
CTHR: 
TMN: 
FGR: 
NHE: 
TRE: 
MAW: 
MAJ: 
CMT: 
VAL: 
ELA: 
SSL: 
BFU: 
MBE: 
ANI: 
AFM: 
AOR: 
AOR_1_1728154(AO090003000972)
ANG: 
ANI_1_512164(An18g03980)
AFV: 
ACT: 
NFI: 
PCS: 
CIM: 
CPW: 
PBL: 
URE: 
ABE: 
TVE: 
AJE: 
PNO: 
PTE: 
BZE: 
BSC: 
BOR: 
ZTR: 
PFJ: 
BCOM: 
NPA: 
TML: 
CGI: 
TMS: 
DSQ: 
PCO: 
SHS: 
PSQ: 
FME: 
GTR: 
LBC: 
MPR: 
MRR: 
CCI: 
SCM: 
ABP: 
AGABI1DRAFT106593(AGABI1DRAFT_106593) AGABI1DRAFT72743(AGABI1DRAFT_72743)
ABV: 
AGABI2DRAFT146446(AGABI2DRAFT_146446) AGABI2DRAFT219485(AGABI2DRAFT_219485)
CPUT: 
SLA: 
UMA: 
PFP: 
MGL: 
MLR: 
ACAN: 
PIF: 
EHX: 
GTT: 
XCI: 
XAO: 
XFU: 
RHD: 
DJI: 
DJA: 
RHA: 
SEN: 
ABA: 
ACA: 
ACM: 
GMA: 
TSA: 
TRS: 
SUS: 
CTM: 
GAU: 
GBA: 
SACI: 
GLJ: 
RMR: 
RMG: 
CPI: 
SHG: 
CMR: 
BBD: 
DFE: 
SLI: 
RSI: 
EOL: 
RBI: 
MRS: 
PTQ: 
FGI: 
 » show all
Taxonomy
Reference
1  [PMID:9123729]
  Authors
Heston WD.
  Title
Characterization and glutamyl preferring carboxypeptidase function of prostate specific membrane antigen: a novel folate hydrolase.
  Journal
Urology. 49 (1997) 104-12.
Reference
2  [PMID:9187245]
  Authors
Rawlings ND, Barrett AJ.
  Title
Structure of membrane glutamate carboxypeptidase.
  Journal
Biochim. Biophys. Acta. 1339 (1997) 247-52.
  Sequence
[hsa:2346]
Reference
3  [PMID:9685395]
  Authors
Halsted CH, Ling EH, Luthi-Carter R, Villanueva JA, Gardner JM, Coyle JT.
  Title
Folylpoly-gamma-glutamate carboxypeptidase from pig jejunum. Molecular characterization and relation to glutamate carboxypeptidase II.
  Journal
J. Biol. Chem. 273 (1998) 20417-24.
  Sequence
[ssc:397677]
Reference
4  [PMID:9501243]
  Authors
Luthi-Carter R, Berger UV, Barczak AK, Enna M, Coyle JT.
  Title
Isolation and expression of a rat brain cDNA encoding glutamate carboxypeptidase II.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 95 (1998) 3215-20.
  Sequence
[rno:85309]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9074-87-7

DBGET integrated database retrieval system