KEGG   ENZYME: 3.4.19.12Help
Entry
EC 3.4.19.12                Enzyme                                 

Name
ubiquitinyl hydrolase 1;
ubiquitin C-terminal hydrolase;
yeast ubiquitin hydrolase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Omega peptidases
BRITE hierarchy
Reaction(IUBMB)
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal)
Comment
Links to polypeptides smaller than 60 residues are hydrolysed more readily than those to larger polypeptides. Isoforms exist with quantitatively different specificities, amongst the best known being UCH-L1 and UCH-L3, which are major proteins of the brain of mammals [1]. Inhibited by ubiquitin aldehyde (in which Gly76 is replaced by aminoacetaldehyde). Ubiquitinyl hydrolase 1 is the type example of peptidase family C12, with a similar protein fold to papain and catalytic amino acids Cys, His and Asp. There is a separate family (C19) of enzymes that also hydrolyse ubiquitinyl bonds, and it is thought that all the ubiquitinyl hydrolases are also ubiquitin thiolesterases (EC 3.1.2.15)
History
EC 3.4.19.12 created 2000
Orthology
K05609  
ubiquitin carboxyl-terminal hydrolase L3
K05610  
ubiquitin carboxyl-terminal hydrolase L5
K05611  
ubiquitin carboxyl-terminal hydrolase L1
K08588  
ubiquitin carboxyl-terminal hydrolase BAP1
K15235  
josephin
Genes
HSA: 
126119(JOSD2) 51377(UCHL5) 7345(UCHL1) 7347(UCHL3) 8314(BAP1) 9929(JOSD1)
PTR: 
100611316(UCHL3) 457596(UCHL5) 458834(JOSD1) 460426(BAP1) 461185(UCHL1) 747924(JOSD2)
PPS: 
GGO: 
101125016(UCHL5) 101125045(BAP1) 101131709(JOSD1) 101136267(JOSD2) 101138427(UCHL3) 101150793(UCHL1)
PON: 
100173411(JOSD1) 100174635(UCHL5) 100440914(UCHL1) 100452766(UCHL3) 100458998(ASPDH) 100462322(BAP1) 100936578(JOSD2)
MCC: 
697221(BAP1) 697276(UCHL3) 701426(JOSD1) 701579 712473 719609
MCF: 
101867495 102117209(UCHL3) 102117932(BAP1) 102125403(UCHL5) 102128408(JOSD2) 102133043(UCHL1)
MMU: 
100043771(Gm10651) 104416(Bap1) 22223(Uchl1) 50933(Uchl3) 56207(Uchl5) 66124(Josd2) 74158(Josd1) 93841(Uchl4)
RNO: 
292876(Josd2) 29545(Uchl1) 306257(Bap1) 315134(Josd1) 360853(Uchl5) 498560(Uchl3)
CGE: 
HGL: 
101696695(Uchl5) 101702892(Uchl1) 101703932(Bap1) 101704183(Josd1) 101718538(Uchl3) 101725004(Josephin-2)
TUP: 
102478017(UCHL1) 102478912(UCHL3) 102485260(JOSD2) 102497298(JOSD1) 102497774(UCHL5) 102498644(BAP1)
CFA: 
476947(UCHL3) 478958(UCHL5) 479098(UCHL1) 481251(JOSD1) 484737(BAP1) 611845(JOSD2)
AML: 
FCA: 
101081646(JOSD1) 101083135(UCHL3) 101085935(JOSD2) 101087295 101092671(BAP1) 101093604(UCHL1)
PTG: 
102949487(UCHL1) 102958964(UCHL5) 102959486(BAP1) 102961717(JOSD2) 102965643(UCHL3) 102970403(JOSD1)
BTA: 
100124510(BAP1) 100848343 282110(UCHL5) 510781(JOSD1) 514394(UCHL1) 520170(UCHL3)
BOM: 
102264734(UCHL3) 102264871(JOSD1) 102272096(BAP1) 102272289(UCHL5) 102282378(UCHL1) 102288102(JOSD2)
PHD: 
102317147(UCHL3) 102325635(UCHL1) 102327814(BAP1) 102336506(JOSD2) 102339601(UCHL5) 102342375(JOSD1)
CHX: 
102175982(JOSD2) 102178714(UCHL1) 102182142(UCHL3) 102182955(JOSD1) 102183466(BAP1) 102191821(UCHL5)
SSC: 
100144472(UCHL5) 100154798(BAP1) 100511033 100520958(JOSD1) 100627842(JOSD2) 396637(UCHL1) 768115(UCHL3)
CFR: 
102507883(UCHL5) 102510412(JOSD1) 102514352(UCHL1) 102514794(BAP1) 102517235(JOSD2) 102522547(UCHL3)
ECB: 
100033838(UCHL1) 100050355 100051934(BAP1) 100052000(UCHL3) 100055255(JOSD1) 100059547(UCHL5)
MYB: 
102244961 102247419(JOSD2) 102249204(UCHL5) 102249893(BAP1) 102250549(UCHL3) 102255672(JOSD1) 102262310(UCHL1)
MYD: 
102767238(JOSD1) 102768770(BAP1) 102769091(UCHL1) 102774379(UCHL5) 102774738(UCHL3)
PALE: 
102877838(UCHL5) 102877993(UCHL3) 102878078(JOSD1) 102895201(JOSD2) 102898027(BAP1)
MDO: 
SHR: 
100914482(JOSD1) 100918150(UCHL1) 100929063 100931031(BAP1) 100932272(UCHL5)
OAA: 
GGA: 
395626(UCHL3) 415944(BAP1) 418023(JOSD1) 424359(UCHL5) 770302(UCHL1)
MGP: 
TGU: 
100222923(UCHL5) 100224993(BAP1) 100225356(UCHL3) 100230150(JOSD1) 751589(UCHL1)
FAB: 
101807637(UCHL5) 101809666(UCHL1) 101812208(UCHL3) 101816494(JOSD1) 101821997(BAP1)
PHI: 
102100040(UCHL3) 102104928(UCHL1) 102107539(BAP1) 102108302(JOSD2) 102109091(UCHL5) 102113325(JOSD1)
APLA: 
101789443 101793188(UCHL3) 101796680(JOSD1) 101799480(UCHL1) 101803525(BAP1) 101804004(UCHL5)
FPG: 
101911799 101916749(UCHL3) 101918204(UCHL5) 101920162(JOSD1) 101921900(BAP1) 101924718(UCHL1)
FCH: 
102046254(UCHL3) 102047483(UCHL1) 102048281(JOSD1) 102051070(JOSD2) 102058192(UCHL5) 102058766(BAP1)
CLV: 
102083724(UCHL1) 102093813(BAP1) 102094651(UCHL3) 102096661(UCHL5) 102097908(JOSD1)
ASN: 
102368341(UCHL3) 102369399(BAP1) 102372366 102373232(UCHL5) 102373248(JOSD1) 102384982(JOSD2)
PSS: 
102448319(JOSD1) 102451446(UCHL1) 102459880(UCHL5)
CMY: 
102930446(UCHL3) 102930669(JOSD1) 102938034(BAP1) 102940171(UCHL5) 102945907(UCHL1)
ACS: 
XLA: 
495025(uchl3) 733154(uchl5) 734333(josd2) 734438(bap1) 734985(josd1) 735204(uchl1)
XTR: 
100124308(uchl5) 100145372 100158575(josd1) 493568(bap1) 496780(uchl1) 549874(uchl3) 733548(josd2)
DRE: 
100536467 325119(uchl1) 393120(josd2) 406357(uchl5) 554104(uchl3) 558885(bap1) 767655(josd1)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
102350317(UCHL5) 102351136 102358757(JOSD1) 102363406(UCHL3) 102364413(BAP1) 102365585(UCHL1)
BFO: 
CIN: 
SPU: 
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
AGA: 
AAG: 
CQU: 
AME: 
409387(Uch) 412644(GB13002) 413813(Uch-L3) 725780(GB19955)
NVI: 
TCA: 
BMOR: 
API: 
PHU: 
ISC: 
CEL: 
CBR: 
CBG00486 CBG13346(Cbr-ubh-4) CBG18953(Cbr-ubh-3) CBG18954(Cbr-ubh-1) CBG18955
BMY: 
LOA: 
TSP: 
SMM: 
NVE: 
HMG: 
TAD: 
AQU: 
ATH: 
AT1G65650(UCH2) AT2G29640(JOSL) AT4G17510(UCH3) AT5G16310(UCH1)
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
TCC: 
GMX: 
MTR: 
CAM: 
FVE: 
CSV: 
RCU: 
POP: 
POPTR_0001s25650g(POPTRDRAFT_172383) POPTR_0003s07890g(POPTRDRAFT_817181) POPTR_0004s13540g(POPTRDRAFT_800674) POPTR_0017s11160g(POPTRDRAFT_736198)
VVI: 
SLY: 
SOT: 
OSA: 
DOSA: 
Os02t0180100-01(Os02g0180100) Os02t0654500-01(Os02g0654500) Os02t0822200-01(Os02g0822200) Os03t0265200-01(Os03g0265200) Os04t0667500-01(Os04g0667500)
OBR: 
BDI: 
SBI: 
SORBI_01g040140(SORBIDRAFT_01g040140) SORBI_01g042110(SORBIDRAFT_01g042110) SORBI_04g033120(SORBIDRAFT_04g033120) SORBI_06g024310(SORBIDRAFT_06g024310) SORBI_07g023880(SORBIDRAFT_07g023880)
ZMA: 
100191319(si660005b01(122)) 100272329(cl23489_1b) 100282423(IDP92) 100282795 100381623 100382000(pco127746)
SITA: 
ATR: 
s00029p00222940(AMTR_s00029p00222940) s00067p00092640(AMTR_s00067p00092640) s00111p00123040(AMTR_s00111p00123040)
SMO: 
PPP: 
CRE: 
VCN: 
OLU: 
OTA: 
MIS: 
MPP: 
CSL: 
CVR: 
CME: 
GSL: 
CCP: 
SCE: 
YJR099W(YUH1)
AGO: 
ERC: 
KLA: 
LTH: 
PPA: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0H00530(NCAS0H00530)
NDI: 
NDAI_0D00480(NDAI0D00480)
TPF: 
TPHA_0E00500(TPHA0E00500)
TBL: 
TBLA_0F02610(TBLA0F02610)
TDL: 
TDEL_0C03330(TDEL0C03330)
KAF: 
KAFR_0G00890(KAFR0G00890)
DHA: 
PIC: 
PGU: 
LEL: 
CAL: 
CaO19.11412(UCT99) CaO19.13651(YUH1) CaO19.3930(UCT99) CaO19.6272(YUH1)
CTP: 
CDU: 
COT: 
YLI: 
CLU: 
NCR: 
SMP: 
PAN: 
TTT: 
MTM: 
MGR: 
FGR: 
NHE: 
TRE: 
VAL: 
SSL: 
BFU: 
ANI: 
NFI: 
AFM: 
AOR: 
AOR_1_1210164(AO090001000670) AOR_1_1916174(AO090005001099) AOR_1_364024(AO090010000205)
ANG: 
ANI_1_1926024(An02g13920)
AFV: 
ACT: 
PCS: 
CIM: 
CPW: 
PBL: 
URE: 
ABE: 
TVE: 
AJE: 
PNO: 
PTE: 
ZTR: 
TML: 
SPO: 
CNE: 
CNB: 
CGI: 
PPL: 
LBC: 
MPR: 
CCI: 
SCM: 
UMA: 
MGL: 
PGR: 
MBR: 
DDI: 
DPP: 
DFA: 
EHI: 
EHI_140500(60.t00040)
EDI: 
ACAN: 
PFA: 
PFD: 
PFH: 
PYO: 
PCB: 
PBE: 
PKN: 
PVX: 
PCY: 
BBO: 
BBOV_III010630(17.m10634)
CPV: 
CHO: 
TGO: 
TET: 
PTM: 
PTI: 
TPS: 
PIF: 
NGD: 
EHX: 
GTT: 
TBR: 
TCR: 
LMA: 
LIF: 
LDO: 
LMI: 
LBZ: 
NGR: 
TVA: 
 » show all
Taxonomy
Reference
1  [PMID:9233788]
  Authors
Johnston SC, Larsen CN, Cook WJ, Wilkinson KD, Hill CP.
  Title
Crystal structure of a deubiquitinating enzyme (human UCH-L3) at 1.8 A resolution.
  Journal
EMBO. J. 16 (1997) 3787-96.
  Organism
Homo sapiens
  Sequence
[hsa:7347]
Reference
2
  Authors
Wilkinson, K.D.
  Title
Ubiquitin C-terminal hydrolase.
  Journal
In: Barrett, A.J., Rawlings, N.D. and Woessner, J.F. (Eds.), Handbook of Proteolytic Enzymes, Academic Press, London, 1998, p. 470-472.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
86480-67-3 189642-63-5

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