KEGG   ENZYME: 3.4.21.100Help
Entry
EC 3.4.21.100               Enzyme                                 

Name
sedolisin;
Pseudomonas sp. pepstatin-insensitive carboxyl proteinase;
pseudomonapepsin;
pseudomonalisin;
sedolysin
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of the B chain of insulin at -Glu13!Ala-, -Leu15!Tyr- and -Phe25!Tyr-, and angiotensin I at -Tyr4!Ile-. A good synthetic substrate is Lys-Pro-Ile-Glu-Phe!Phe(NO2)-Arg-Leu.
Comment
An enzyme secreted by Pseudomonas sp. No. 101. Optimum pH is 4. It is distinguished from xanthomonapepsin by its insensitivity to EPNP and from scytalidopepsin B by this property and by its unrelated amino-acid sequence. Inhibited by tyrostatin, a peptide aldehyde [2]. Type example of peptidase family S53.
History
EC 3.4.21.100 created 1995 as EC 3.4.23.37, transferred 2001 to EC 3.4.21.100, modified 2003
Orthology
K05998  
pseudomonalisin
Genes
XAL: 
FAU: 
DJA: 
CVI: 
BMA: 
BMV: 
BML: 
BMN: 
BPS: 
BPM: 
BPL: 
BPD: 
BPR: 
BPSE: 
BDL_3405(pcp) BDL_5091(pcp)
BPSM: 
BBQ_1194(pcp) BBQ_4404(pcp)
BPSU: 
BBN_1320(pcp) BBN_5191(pcp)
BPSD: 
BBX_1806(pcp) BBX_4257(pcp)
BPZ: 
BPQ: 
BPK: 
BBK_2822(pcp) BBK_4455(pcp)
BTE: 
BTQ: 
BTQ_1866(pcp) BTQ_3938(pcp)
BTJ: 
BTJ_489(pcp) BTJ_4970(pcp)
BTZ: 
BTL_1729(pcp) BTL_3439(pcp)
BTD: 
BVI: 
BUR: 
BCN: 
BCH: 
BCM: 
BCJ: 
BAM: 
BAC: 
BMU: 
BMJ: 
BCT: 
BGL: 
BGD: 
BUK: 
CFU: 
 » show all
Taxonomy
Reference
1  [PMID:3548827]
  Authors
Oda K, Sugitani M, Fukuhara K, Murao S.
  Title
Purification and properties of a pepstatin-insensitive carboxyl proteinase from a gram-negative bacterium.
  Journal
Biochim. Biophys. Acta. 923 (1987) 463-9.
Reference
2  [PMID:1562589]
  Authors
Oda K, Nakatani H, Dunn BM.
  Title
Substrate specificity and kinetic properties of pepstatin-insensitive carboxyl proteinase from Pseudomonas sp. No. 101.
  Journal
Biochim. Biophys. Acta. 1120 (1992) 208-14.
Reference
3  [PMID:11323721]
  Authors
Wlodawer A, Li M, Dauter Z, Gustchina A, Uchida K, Oyama H, Dunn BM, Oda K.
  Title
Carboxyl proteinase from Pseudomonas defines a novel family of subtilisin-like enzymes.
  Journal
Nat. Struct. Biol. 8 (2001) 442-6.
  Sequence
[up:P42790]
Reference
4  [PMID:12673349]
  Authors
Wlodawer A, Li M, Gustchina A, Oyama H, Dunn BM, Oda K.
  Title
Structural and enzymatic properties of the sedolisin family of serine-carboxyl peptidases.
  Journal
Acta. Biochim. Pol. 50 (2003) 81-102.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
848318-58-1

DBGET integrated database retrieval system