KEGG   ENZYME: 3.4.21.100Help
Entry
EC 3.4.21.100               Enzyme                                 
Name
sedolisin;
Pseudomonas sp. pepstatin-insensitive carboxyl proteinase;
pseudomonapepsin;
pseudomonalisin;
sedolysin
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Serine endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of the B chain of insulin at -Glu13!Ala-, -Leu15!Tyr- and -Phe25!Tyr-, and angiotensin I at -Tyr4!Ile-. A good synthetic substrate is Lys-Pro-Ile-Glu-Phe!Phe(NO2)-Arg-Leu.
Comment
An enzyme secreted by Pseudomonas sp. No. 101. Optimum pH is 4. It is distinguished from xanthomonapepsin by its insensitivity to EPNP and from scytalidopepsin B by this property and by its unrelated amino-acid sequence. Inhibited by tyrostatin, a peptide aldehyde [2]. Type example of peptidase family S53.
Orthology
K05998  
pseudomonalisin
Genes
XAL: 
FAU: 
CVI: 
BMA: 
BMV: 
BML: 
BMN: 
BPS: 
BPM: 
BPL: 
BPD: 
BURPS668_2417 BURPS668_A2497 BURPS668_A2829
BPR: 
GBP346_A2543
BPZ: 
BP1026B_I1253 BP1026B_II1859
BPQ: 
BPC006_I2516 BPC006_II2327
BTE: 
BTH_II0646
BVI: 
Bcep1808_4399
BUR: 
Bcep18194_B2163 Bcep18194_B2199
BCN: 
Bcen_4443 Bcen_4475
BCH: 
Bcen2424_3891 Bcen2424_3924
BCM: 
Bcenmc03_3604 Bcenmc03_3636
BCJ: 
BCAM0922 BCAM0957
BAM: 
Bamb_3301
BAC: 
BamMC406_3792 BamMC406_3817
BMU: 
Bmul_4660
BMJ: 
BMULJ_03854
BCT: 
GEM_4830 GEM_4861
BGL: 
bglu_2g07550
BGD: 
bgla_2g18790
BUK: 
MYA_4042
CFU: 
CFU_2834
 » show all
Taxonomy
Reference
1  [PMID:3548827]
  Authors
Oda K, Sugitani M, Fukuhara K, Murao S.
  Title
Purification and properties of a pepstatin-insensitive carboxyl proteinase from a gram-negative bacterium.
  Journal
Biochim. Biophys. Acta. 923 (1987) 463-9.
Reference
2  [PMID:1562589]
  Authors
Oda K, Nakatani H, Dunn BM.
  Title
Substrate specificity and kinetic properties of pepstatin-insensitive carboxyl proteinase from Pseudomonas sp. No. 101.
  Journal
Biochim. Biophys. Acta. 1120 (1992) 208-14.
  Organism
Pseudomonas sp.
Reference
3  [PMID:11323721]
  Authors
Wlodawer A, Li M, Dauter Z, Gustchina A, Uchida K, Oyama H, Dunn BM, Oda K.
  Title
Carboxyl proteinase from Pseudomonas defines a novel family of subtilisin-like enzymes.
  Journal
Nat. Struct. Biol. 8 (2001) 442-6.
  Organism
Pseudomonas sp.
Reference
4  [PMID:12673349]
  Authors
Wlodawer A, Li M, Gustchina A, Oyama H, Dunn BM, Oda K.
  Title
Structural and enzymatic properties of the sedolisin family of serine-carboxyl peptidases.
  Journal
Acta. Biochim. Pol. 50 (2003) 81-102.
  Organism
Pseudomonas sp.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
848318-58-1
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