KEGG   ENZYME: 3.4.23.12Help
Entry
EC 3.4.23.12                Enzyme                                 

Name
nepenthesin;
Nepenthes aspartic proteinase;
Nepenthes acid proteinase;
nepenthacin;
nepenthasin;
aspartyl endopeptidase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Aspartic endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Similar to pepsin, but also cleaves on either side of Asp and at Lys!Arg
Comment
From the insectivorous plants Nepenthes spp. (secretions) and Drosera peltata (ground-up leaves). Aspartic endopeptidases are probably present in many other plants, including Lotus [3] and sorghum [2]. In peptidase family A1 (pepsin A family)
History
EC 3.4.23.12 created 1972 as EC 3.4.99.4, transferred 1978 to EC 3.4.23.12, modified 1981
Reference
1  [PMID:5354017]
  Authors
Amagase S, Nakayama S, Tsugita A.
  Title
Acid protease in Nepenthes. II. Study on the specificity of nepenthesin.
  Journal
J. Biochem. (Tokyo). 66 (1969) 431-9.
  Organism
Nepenthes sp.
Reference
2  [PMID:5486576]
  Authors
Garg GK, Virupaksha TK.
  Title
Acid protease from germinated sorghum. 2. Substrate specificity with synthetic peptides and ribonuclease A.
  Journal
Eur. J. Biochem. 17 (1970) 13-8.
Reference
3
  Authors
Shinano, S. and Fukushima, K.
  Title
Studies on lotus seed protease. Part III. Some physicochemical and enzymic properties.
  Journal
Agric. Biol. Chem. 35 (1971) 1488-1494.
Reference
4  [PMID:5069751]
  Authors
Amagase S.
  Title
Digestive enzymes in insectivorous plants. 3. Acid proteases in the genus Nepenthes and Drosera peltata.
  Journal
J. Biochem. (Tokyo). 72 (1972) 73-81.
  Organism
Drosera peltata, Nepenthes sp.
Reference
5  [PMID:4436292]
  Authors
Takahashi K, Chang W, Ko J.
  Title
Specific inhibition of acid proteases from brain, kidney, skeletal muscle, and insectivorous plants by diazoacetyl-DL-norleucine methyl ester and by pepstatin.
  Journal
J. Biochem. (Tokyo). 76 (1974) 897-9.
  Organism
Nepenthes macferlanei
Reference
6  [PMID:16526095]
  Authors
Coiras M, Camafeita E, Urena T, Lopez JA, Caballero F, Fernandez B, Lopez-Huertas MR, Perez-Olmeda M, Alcami J.
  Title
Modifications in the human T cell proteome induced by intracellular HIV-1 Tat protein expression.
  Journal
Proteomics. 6 Suppl 1 (2006) S63-73.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9073-80-7

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