KEGG   ENZYME: 3.4.23.20Help
Entry
EC 3.4.23.20                Enzyme                                 

Name
penicillopepsin;
peptidase A;
Penicillium janthinellum aspartic proteinase;
acid protease A;
Penicillium citrinum acid proteinase;
Penicillium cyclopium acid proteinase;
Penicillium expansum acid proteinase;
Penicillium janthinellum acid proteinase;
Penicillium expansum aspartic proteinase;
Penicillium aspartic proteinase;
Penicillium caseicolum aspartic proteinase;
Penicillium roqueforti acid proteinase;
Penicillium duponti aspartic proteinase;
Penicillium citrinum aspartic proteinase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Aspartic endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of proteins with broad specificity similar to that of pepsin A, preferring hydrophobic residues at P1 and P1', but also cleaving Gly20!Glu in the B chain of insulin. Clots milk, and activates trypsinogen
Comment
From the imperfect fungus Penicillium janthinellum. In peptidase family A1 (pepsin A family). Closely related enzymes have been isolated from P. roqueforti [2] and P. duponti [3].
History
EC 3.4.23.20 created 1992 (EC 3.4.23.6 created 1992 (EC 3.4.23.6 created 1961 as EC 3.4.4.17, transferred 1972 to EC 3.4.23.6, modified 1981 [EC 3.4.23.7, EC 3.4.23.8, EC 3.4.23.9, EC 3.4.23.10, EC 3.4.99.1, EC 3.4.99.15 and EC 3.4.99.25 all created 1972 and incorporated 1978], part incorporated 1992)
Reference
1  [PMID:4946839]
  Authors
Mains G, Takahashi M, Sodek J, Hofmann T.
  Title
The specificity of penicillopepsin.
  Journal
Can. J. Biochem. 49 (1971) 1134-49.
Reference
2  [PMID:4790849]
  Authors
Zevaco C, Hermier J, Gripon JC.
  Title
[Proteolytic system in Penicillium roqueforti. 2. Purification and properties of acid protease]
  Journal
Biochimie. 55 (1973) 1353-60.
Reference
3  [PMID:2287]
  Authors
Emi S, Myers DV, Iacobucci GA.
  Title
Purification and properties of the thermostable acid protease of Penicillium duponti.
  Journal
Biochemistry. 15 (1976) 842-8.
Reference
4  [PMID:1012008]
  Authors
Hofmann T.
  Title
Penicillopepsin.
  Journal
Methods. Enzymol. 45 (1976) 434-52.
Reference
5  [PMID:323722]
  Authors
Hsu IN, Delbaere LT, James MN, Hofmann T.
  Title
Penicillopepsin from Penicillium janthinellum crystal structure at 2.8 A and sequence homology with porcine pepsin.
  Journal
Nature. 266 (1977) 140-5.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9074-08-2

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