KEGG   ENZYME: 3.4.23.21Help
Entry
EC 3.4.23.21                Enzyme                                 

Name
rhizopuspepsin;
Rhizopus aspartic proteinase;
neurase;
Rhizopus acid protease;
Rhizopus acid proteinase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Aspartic endopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of proteins with broad specificity similar to that of pepsin A, preferring hydrophobic residues at P1 and P1'. Clots milk and activates trypsinogen. Does not cleave Gln4-His, but does cleave His10!Leu and Val12!Glu in B chain of insulin
Comment
From the zygomycete fungus Rhizopus chinensis. A similar endopeptidase is found in R. niveus [2]. In peptidase family A1 (pepsin A family).
History
EC 3.4.23.21 created 1992 (EC 3.4.23.6 created 1992 (EC 3.4.23.6 created 1961 as EC 3.4.4.17, transferred 1972 to EC 3.4.23.6, modified 1981 [EC 3.4.23.7, EC 3.4.23.8, EC 3.4.23.9, EC 3.4.23.10, EC 3.4.99.1, EC 3.4.99.15 and EC 3.4.99.25 all created 1972 and incorporated 1978], part incorporated 1992)
Reference
1
  Authors
Tsuru, D., Hattori, A., Tsuji, H., Yamamoto, T. and Fukumoto, J.
  Title
Studies on mold proteases. Part II. Substrate specificity of acid protease of Rhizopus chinensis.
  Journal
Agric. Biol. Chem. 33 (1969) 1419-1426.
Reference
2
  Authors
Kurono, Y., Chidimatsu, M., Horikoshi, K. and Ikeda, Y.
  Title
Isolation of a protease from a Rhizopus product.
  Journal
Agric. Biol. Chem. 35 (1971) 1668-1675.
Reference
3  [PMID:6751894]
  Authors
Ohtsuru M, Tang J, Delaney R.
  Title
Purification and characterization of rhizopuspepsin isozymes from a liquid culture of Rhizopus chinensis.
  Journal
Int. J. Biochem. 14 (1982) 925-32.
  Organism
Rhizopus chinensis
Reference
4  [PMID:3313384]
  Authors
Suguna K, Padlan EA, Smith CW, Carlson WD, Davies DR.
  Title
Binding of a reduced peptide inhibitor to the aspartic proteinase from Rhizopus chinensis: implications for a mechanism of action.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 84 (1987) 7009-13.
  Organism
Rhizopus chinensis
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9074-09-3

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