KEGG   ENZYME: 3.4.24.33Help
Entry
EC 3.4.24.33                Enzyme                                 

Name
peptidyl-Asp metalloendopeptidase;
endoproteinase Asp-N;
peptidyl-Asp metalloproteinase
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Cleavage of Xaa!Asp, Xaa!Glu and Xaa!cysteic acid bonds
Comment
A metalloenzyme isolated from Pseudomonas fragi. Useful in protein sequencing applications because of its limited specificity. In peptidase family M72.
History
EC 3.4.24.33 created 1992
Reference
1  [PMID:236771]
  Authors
Porzio MA, Pearson AM.
  Title
Isolation of an extracellular neutral proteinase from Pseudomonas fragi.
  Journal
Biochim. Biophys. Acta. 384 (1975) 235-41.
  Organism
Pseudomonas fragi
Reference
2  [PMID:7188696]
  Authors
Drapeau GR.
  Title
Substrate specificity of a proteolytic enzyme isolated from a mutant of Pseudomonas fragi.
  Journal
J. Biol. Chem. 255 (1980) 839-40.
  Organism
Pseudomonas fragi
  Sequence
[up:Q9R4J4]
Reference
3  [PMID:2669754]
  Authors
Ingrosso D, Fowler AV, Bleibaum J, Clarke S.
  Title
Specificity of endoproteinase Asp-N (Pseudomonas fragi): cleavage at glutamyl residues in two proteins.
  Journal
Biochem. Biophys. Res. Commun. 162 (1989) 1528-34.
  Organism
Pseudomonas fragi
  Sequence
[up:Q9R4J4]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
55576-49-3

DBGET integrated database retrieval system