| Entry |
|
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| Name |
peptidyl-Asp metalloendopeptidase;
endoproteinase Asp-N;
peptidyl-Asp metalloproteinase
|
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| Class |
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
 |
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| Reaction(IUBMB) |
Cleavage of Xaa!Asp, Xaa!Glu and Xaa!cysteic acid bonds
|
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| Comment |
A metalloenzyme isolated from Pseudomonas fragi. Useful in protein sequencing applications because of its limited specificity. In peptidase family M72.
|
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| Reference |
|
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| Authors |
Porzio MA, Pearson AM. |
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| Title |
Isolation of an extracellular neutral proteinase from Pseudomonas fragi. |
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| Journal |
Biochim. Biophys. Acta. 384 (1975) 235-41. |
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| Organism |
Pseudomonas fragi |
|---|
| Reference |
|
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| Authors |
Drapeau GR. |
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| Title |
Substrate specificity of a proteolytic enzyme isolated from a mutant of Pseudomonas fragi. |
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| Journal |
J. Biol. Chem. 255 (1980) 839-40. |
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| Organism |
Pseudomonas fragi |
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| Reference |
|
|---|
| Authors |
Ingrosso D, Fowler AV, Bleibaum J, Clarke S. |
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| Title |
Specificity of endoproteinase Asp-N (Pseudomonas fragi): cleavage at glutamyl residues in two proteins. |
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| Journal |
Biochem. Biophys. Res. Commun. 162 (1989) 1528-34. |
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| Organism |
Pseudomonas fragi |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 55576-49-3 |
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