KEGG   ENZYME: 3.4.24.40Help
Entry
EC 3.4.24.40                Enzyme                                 

Name
serralysin;
Pseudomonas aeruginosa alkaline proteinase;
Escherichia freundii proteinase;
Serratia marcescens extracellular proteinase;
Serratia marcescens metalloproteinase;
Pseudomonas aeruginosa alk. protease;
Serratia marcescens metalloprotease
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Preferential cleavage of bonds with hydrophobic residues in P1'
Comment
A 50 kDa extracellular endopeptidase from Pseudomonas aeruginosa [1,2,6], Escherichia freundii [3], Serratia marcescens [4,5,6] and Erwinia chrysanthemi [7]. There is broad specificity in cleavage of the insulin B chain, with some species variations. The pH optimum for digesting various proteins is about 9 - 10. In peptidase family M10 (interstitial collagenase family). Formerly included in EC 3.4.24.4
History
EC 3.4.24.40 created 1972 as EC 3.4.24.4, part transferred 1992 to EC 3.4.24.40
Orthology
K01406  
serralysin
Genes
YPP: 
SPE: 
PSB: 
PFL: 
PFL_3210(aprA)
PEN: 
PSEEN1550(aprA)
PMY: 
RSH: 
MMR: 
AVA: 
Taxonomy
Reference
1  [PMID:4967801]
  Authors
Morihara K, Tsuzuki H, Oka T.
  Title
Comparison of the specificities of various neutral proteinases from microorganisms.
  Journal
Arch. Biochem. Biophys. 123 (1968) 572-88.
Reference
2  [PMID:4199986]
  Authors
Morihara K, Tsuzuki H, Oka T.
  Title
On the specificity of Pseudomonas aeruginosa alkaline proteinase with synthetic peptides.
  Journal
Biochim. Biophys. Acta. 309 (1973) 414-29.
  Sequence
[pae:PA1249]
Reference
3  [PMID:4212288]
  Authors
Nakajima M, Mizusawa K, Yoshida F.
  Title
Purification and properties of an extracellular proteinase of psychrophilic Escherichia freundii.
  Journal
Eur. J. Biochem. 44 (1974) 87-96.
Reference
4  [PMID:383155]
  Authors
Decedue CJ, Broussard EA 2nd, Larson AD, Braymer HD.
  Title
Purification and characterization of the extracellular proteinase of Serratia marcescens.
  Journal
Biochim. Biophys. Acta. 569 (1979) 293-301.
Reference
5  [PMID:6380155]
  Authors
Doerr M, Traub WH.
  Title
Purification and characterization of two Serratia marcescens proteases.
  Journal
Zentralbl. Bakteriol. Mikrobiol. Hyg. [A]. 257 (1984) 6-19.
Reference
6  [PMID:3016665]
  Authors
Nakahama K, Yoshimura K, Marumoto R, Kikuchi M, Lee IS, Hase T, Matsubara H.
  Title
Cloning and sequencing of Serratia protease gene.
  Journal
Nucleic. Acids. Res. 14 (1986) 5843-55.
  Sequence
[smaf:D781_0184]
Reference
7  [PMID:2211513]
  Authors
Dahler GS, Barras F, Keen NT.
  Title
Cloning of genes encoding extracellular metalloproteases from Erwinia chrysanthemi EC16.
  Journal
J. Bacteriol. 172 (1990) 5803-15.
  Sequence
[up:P19144]
Reference
8  [PMID:2123832]
  Authors
Okuda K, Morihara K, Atsumi Y, Takeuchi H, Kawamoto S, Kawasaki H, Suzuki K, Fukushima J.
  Title
Complete nucleotide sequence of the structural gene for alkaline proteinase from Pseudomonas aeruginosa IFO 3455.
  Journal
Infect. Immun. 58 (1990) 4083-8.
  Sequence
[pae:PA1249]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
70851-98-8

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