KEGG   ENZYME: 3.4.24.42Help
Entry
EC 3.4.24.42                Enzyme                                 

Name
atrolysin C;
Crotalus atrox metalloendopeptidase c;
hemorrhagic toxin c and d
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Cleavage of His5!Leu, His10!Leu, Ala14!Leu, Tyr16!Leu and Gly23!Phe bonds in B chain of insulin. With small molecule substrates prefers hydrophobic residue at P2' and small residue such as Ala, Gly at P1
Comment
A 24 kDa hemorrhagic endopeptidase from the venom of the western diamondback rattlesnake (Crotalus atrox) that digests type IV collagen, and exists as two forms, c and d. Phosphoramidon inhibits in the 0.1 mM range. In peptidase family M12 (astacin family). Hemorrhagic toxin-2 of C. ruber ruber has the same Mr and specificity and is a homologue [4,6].
History
EC 3.4.24.42 created 1992
Reference
1  [PMID:210790]
  Authors
Bjarnason JB, Tu AT.
  Title
Hemorrhagic toxins from Western diamondback rattlesnake (Crotalus atrox) venom: isolation and characterization of five toxins and the role of zinc in hemorrhagic toxin e.
  Journal
Biochemistry. 17 (1978) 3395-404.
  Organism
Crotalus atrox
Reference
2  [PMID:3514216]
  Authors
Fox JW, Campbell R, Beggerly L, Bjarnason JB.
  Title
Substrate specificities and inhibition of two hemorrhagic zinc proteases Ht-c and Ht-d from Crotalus atrox venom.
  Journal
Eur. J. Biochem. 156 (1986) 65-72.
  Organism
Crotalus atrox
Reference
3  [PMID:3101740]
  Authors
Bjarnason JB, Fox JW.
  Title
Characterization of two hemorrhagic zinc proteinases, toxin c and toxin d, from western diamondback rattlesnake (Crotalus atrox) venom.
  Journal
Biochim. Biophys. Acta. 911 (1987) 356-63.
  Organism
Crotalus atrox
Reference
4  [PMID:2949699]
  Authors
Mori N, Nikai T, Sugihara H, Tu AT.
  Title
Biochemical characterization of hemorrhagic toxins with fibrinogenase activity isolated from Crotalus ruber ruber venom.
  Journal
Arch. Biochem. Biophys. 253 (1987) 108-21.
  Organism
Crotalus ruber ruber
Reference
5  [PMID:2745407]
  Authors
Shannon JD, Baramova EN, Bjarnason JB, Fox JW.
  Title
Amino acid sequence of a Crotalus atrox venom metalloproteinase which cleaves type IV collagen and gelatin.
  Journal
J. Biol. Chem. 264 (1989) 11575-83.
  Organism
Crotalus atrox
  Sequence
[up:P15167]
Reference
6  [PMID:2081731]
  Authors
Takeya H, Onikura A, Nikai T, Sugihara H, Iwanaga S.
  Title
Primary structure of a hemorrhagic metalloproteinase, HT-2, isolated from the venom of Crotalus ruber ruber.
  Journal
J. Biochem. (Tokyo). 108 (1990) 711-9.
  Organism
Crotalus ruber ruber
  Sequence
[up:P20897]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
158886-17-0

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