KEGG   ENZYME: 3.4.24.65Help
Entry
EC 3.4.24.65                Enzyme                                 
Name
macrophage elastase;
metalloelastase;
human macrophage metalloelastase (HME)
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of soluble and insoluble elastin [1]. Specific cleavages are also produced at -Ala14!Leu- and -Tyr16!Leu- in the B chain of insulin [2]
Comment
This enzyme is synthesized as a proenzyme of 53 kDa that is converted to an active form of 22 kDa. cDNA sequences have been obtained for the mouse [3] and human [4] enzymes. In peptidase family M10 (interstitial collagenase family)
Orthology
K01413  
matrix metalloproteinase-12 (macrophage elastase)
Genes
HSA: 
4321(MMP12)
PTR: 
451512(MMP12)
PPS: 
100994913(MMP12)
GGO: 
101133109(MMP12)
PON: 
MCC: 
703867(MMP12)
MMU: 
17381(Mmp12)
RNO: 
117033(Mmp12)
CFA: 
611789(MMP12)
AML: 
FCA: 
101084472(MMP12)
BTA: 
526981(MMP12)
SSC: 
100101475(MMP12)
ECB: 
100069047
MDO: 
100012514
SHR: 
100915322(MMP12)
 » show all
Taxonomy
Reference
1  [PMID:7030312]
  Authors
Banda MJ, Werb Z.
  Title
Mouse macrophage elastase. Purification and characterization as a metalloproteinase.
  Journal
Biochem. J. 193 (1981) 589-605.
  Organism
Mus musculus [GN:mmu]
Reference
2  [PMID:7032505]
  Authors
Kettner C, Shaw E, White R, Janoff A.
  Title
The specificity of macrophage elastase on the insulin B-chain.
  Journal
Biochem. J. 195 (1981) 369-72.
  Organism
Mus musculus [GN:mmu]
Reference
3  [PMID:1537850]
  Authors
Shapiro SD, Griffin GL, Gilbert DJ, Jenkins NA, Copeland NG, Welgus HG, Senior RM, Ley TJ.
  Title
Molecular cloning, chromosomal localization, and bacterial expression of a murine macrophage metalloelastase.
  Journal
J. Biol. Chem. 267 (1992) 4664-71.
  Organism
Mus musculus [GN:mmu]
Reference
4  [PMID:8226919]
  Authors
Shapiro SD, Kobayashi DK, Ley TJ.
  Title
Cloning and characterization of a unique elastolytic metalloproteinase produced by human alveolar macrophages.
  Journal
J. Biol. Chem. 268 (1993) 23824-9.
  Organism
Homo sapiens [GN:hsa]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9004-06-2
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