KEGG   ENZYME: 3.4.24.68Help
Entry
EC 3.4.24.68                Enzyme                                 

Name
tentoxilysin;
tetanus neurotoxin
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Hydrolysis of -Gln76!Phe- bond in synaptobrevin (also known as neuronal vesicle-associated membrane protein, VAMP)
Comment
Zinc enzyme produced by Clostridium tetani. Proenzyme of 150 kDa is processed to disulfide-linked subunits of 100 and 50 kDa, the latter being responsible for the endopeptidase activity. Weakly inhibited by captopril, and phosphoramidon. The clostridial neurotoxins disable the neuroexocytosis apparatus, and have been described as the most toxic substances known. Tentoxilysin acts at the spinal inhibitory interneurons, blocking the release of various neurotransmitters to produce spastic paralysis. Type example of peptidase family M27 (tentoxilysin family)
History
EC 3.4.24.68 created 1995
Orthology
K08644  
tentoxilysin
Genes
CTC: 
pE88_60(tetX)
CTET: 
Taxonomy
Reference
1  [PMID:1376283]
  Authors
Izumi H, Adachi T, Fujii N, Matsuda T, Nakamura R, Tanaka K, Urisu A, Kurosawa Y.
  Title
Nucleotide sequence of a cDNA clone encoding a major allergenic protein in rice seeds. Homology of the deduced amino acid sequence with members of alpha-amylase/trypsin inhibitor family.
  Journal
FEBS. Lett. 302 (1992) 213-6.
  Sequence
[osa:4342722]
Reference
2  [PMID:1331807]
  Authors
Schiavo G, Benfenati F, Poulain B, Rossetto O, Polverino de Laureto P, DasGupta BR, Montecucco C.
  Title
Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevin.
  Journal
Nature. 359 (1992) 832-5.
  Sequence
[ctc:pE88_60]
Reference
3  [PMID:1429690]
  Authors
Schiavo G, Rossetto O, Santucci A, DasGupta BR, Montecucco C.
  Title
Botulinum neurotoxins are zinc proteins.
  Journal
J. Biol. Chem. 267 (1992) 23479-83.
Reference
4  [PMID:7527117]
  Authors
Montecucco C, Schiavo G.
  Title
Mechanism of action of tetanus and botulinum neurotoxins.
  Journal
Mol. Microbiol. 13 (1994) 1-8.
Reference
5  [PMID:7674951]
  Authors
Schiavo G, Montecucco C.
  Title
Tetanus and botulism neurotoxins: isolation and assay.
  Journal
Methods. Enzymol. 248 (1995) 643-52.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
107231-12-9

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