KEGG   ENZYME: 3.4.24.74Help
Entry
EC 3.4.24.74                Enzyme                                 

Name
fragilysin;
Bacteroides fragilis (entero)toxin
Class
Hydrolases;
Acting on peptide bonds (peptidases);
Metalloendopeptidases
BRITE hierarchy
Reaction(IUBMB)
Broad proteolytic specificity, bonds hydrolysed including -Gly!Leu-, -Met!Leu-, -Phe!Leu-, -Cys!Leu-, Leu!Gly
Comment
Thought to be a cause of diarrhoea in animals and humans. Hydrolyses extracellular matrix proteins, and disrupts tight junctions of intestinal epithelial cells. Also degrades intracellular, cytoskeletal proteins actin, myosin and others. In peptidase family M10 (interstitial collagenase family)
History
EC 3.4.24.74 created 1997
Reference
1  [PMID:7806355]
  Authors
Moncrief JS, Obiso R Jr, Barroso LA, Kling JJ, Wright RL, Van Tassell RL, Lyerly DM, Wilkins TD.
  Title
The enterotoxin of Bacteroides fragilis is a metalloprotease.
  Journal
Infect. Immun. 63 (1995) 175-81.
  Organism
Bacteroides fragilis
  Sequence
[up:P54355]
Reference
2  [PMID:7558286]
  Authors
Obiso RJ Jr, Lyerly DM, Van Tassell RL, Wilkins TD.
  Title
Proteolytic activity of the Bacteroides fragilis enterotoxin causes fluid secretion and intestinal damage in vivo.
  Journal
Infect. Immun. 63 (1995) 3820-6.
  Organism
Bacteroides fragilis
Reference
3  [PMID:8557328]
  Authors
Donelli G, Fabbri A, Fiorentini C.
  Title
Bacteroides fragilis enterotoxin induces cytoskeletal changes and surface blebbing in HT-29 cells.
  Journal
Infect. Immun. 64 (1996) 113-9.
  Organism
Bacteroides fragilis
Reference
4  [PMID:8945541]
  Authors
Koshy SS, Montrose MH, Sears CL.
  Title
Human intestinal epithelial cells swell and demonstrate actin rearrangement in response to the metalloprotease toxin of Bacteroides fragilis.
  Journal
Infect. Immun. 64 (1996) 5022-8.
  Organism
Bacteroides fragilis
Reference
5  [PMID:9011050]
  Authors
Kling JJ, Wright RL, Moncrief JS, Wilkins TD.
  Title
Cloning and characterization of the gene for the metalloprotease enterotoxin of Bacteroides fragilis.
  Journal
FEMS. Microbiol. Lett. 146 (1997) 279-84.
  Organism
Bacteroides fragilis
  Sequence
[up:P54355]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
188596-63-6

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