KEGG   ENZYME: 3.5.1.52Help
Entry
EC 3.5.1.52                 Enzyme                                 

Name
peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase;
glycopeptide N-glycosidase;
glycopeptidase;
N-oligosaccharide glycopeptidase;
N-glycanase;
Jack-bean glycopeptidase;
PNGase A;
PNGase F
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
BRITE hierarchy
Sysname
N-linked-glycopeptide-(N-acetyl-beta-D-glucosaminyl)-L-asparagine amidohydrolase
Reaction(IUBMB)
Hydrolysis of an N4-(acetyl-beta-D-glucosaminyl)asparagine residue in which the glucosamine residue may be further glycosylated, to yield a (substituted) N-acetyl-beta-D-glucosaminylamine and a peptide containing an aspartate residue [RN:R08707]
Reaction(KEGG)
Comment
Does not act on (GlcNAc)Asn, because it requires the presence of more than two amino-acid residues in the substrate [cf. EC 3.5.1.26, N4-(beta-N-acetylglucosaminyl)-L-asparaginase]. The plant enzyme was previously erroneously listed as EC 3.2.2.18.
History
EC 3.5.1.52 created 1984, modified 1989 (EC 3.2.2.18 created 1984, incorporated 1989)
Orthology
K01456  
peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase
Genes
HSA: 
55768(NGLY1)
PTR: 
460233(NGLY1)
PPS: 
100990785(NGLY1)
GGO: 
101129625(NGLY1)
PON: 
100438095(NGLY1)
MCC: 
700842(NGLY1)
MCF: 
101925902(NGLY1)
MMU: 
59007(Ngly1)
RNO: 
361014(Ngly1)
CGE: 
100770329(Ngly1)
HGL: 
101696317(Ngly1)
TUP: 
102495051(NGLY1)
CFA: 
100682555(NGLY1)
AML: 
FCA: 
101086171(NGLY1)
PTG: 
102960324(NGLY1)
BTA: 
533736(NGLY1)
BOM: 
102274847(NGLY1)
PHD: 
102333201(NGLY1)
CHX: 
102171687(NGLY1)
SSC: 
100522951(NGLY1)
CFR: 
102522193(NGLY1)
BACU: 
103002175(NGLY1)
LVE: 
103081982(NGLY1)
ECB: 
100059456(NGLY1)
MYB: 
102252924(NGLY1)
MYD: 
PALE: 
102886961(NGLY1)
MDO: 
100031666(NGLY1)
SHR: 
100930637(NGLY1)
OAA: 
100075786(NGLY1)
GGA: 
420655(NGLY1)
MGP: 
TGU: 
100228315(NGLY1)
FAB: 
101814726(NGLY1)
PHI: 
102110753(NGLY1)
APLA: 
101795106(NGLY1)
FPG: 
101913196(NGLY1)
FCH: 
102059937(NGLY1)
CLV: 
102097019(NGLY1)
ASN: 
102383348(NGLY1)
AMJ: 
102571009(NGLY1)
PSS: 
102453461(NGLY1)
CMY: 
102930072(NGLY1)
PBI: 
103064120(NGLY1)
XLA: 
733299(ngly1)
XTR: 
100488447(ngly1)
DRE: 
553627(ngly1)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
102345970(NGLY1)
CMK: 
103179950(ngly1)
BFO: 
CIN: 
SPU: 
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DSE: 
DSI: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
AGA: 
AAG: 
CQU: 
AME: 
409032(PNGase)
NVI: 
TCA: 
BMOR: 
PHU: 
ISC: 
CEL: 
CBR: 
CBG08042(Cbr-png-1)
BMY: 
SMM: 
NVE: 
TAD: 
ATH: 
AT5G49570(PNG1)
ALY: 
CRB: 
EUS: 
CIT: 
CIC: 
TCC: 
GMX: 
PVU: 
MTR: 
CAM: 
FVE: 
PPER: 
CSV: 
RCU: 
POP: 
VVI: 
SLY: 
SOT: 
OSA: 
DOSA: 
Os07t0497400-01(Os07g0497400)
OBR: 
BDI: 
SBI: 
SORBI_02g033840(SORBIDRAFT_02g033840)
SITA: 
ATR: 
s00032p00190520(AMTR_s00032p00190520)
SMO: 
PPP: 
CRE: 
VCN: 
OLU: 
OTA: 
MIS: 
MPP: 
BPG: 
CSL: 
CVR: 
CME: 
CCP: 
SCE: 
YPL096W(PNG1)
AGO: 
ERC: 
KLA: 
LTH: 
PPA: 
VPO: 
ZRO: 
CGR: 
NCS: 
NCAS_0C01930(NCAS0C01930)
NDI: 
NDAI_0E02650(NDAI0E02650)
TPF: 
TPHA_0A01280(TPHA0A01280)
TBL: 
TBLA_0H03830(TBLA0H03830)
TDL: 
TDEL_0A05180(TDEL0A05180)
KAF: 
KAFR_0E00750(KAFR0E00750)
DHA: 
PIC: 
PGU: 
LEL: 
SPAA: 
CAL: 
CaO19.26(PNG1) CaO19.7697(PNG1)
CTP: 
CDU: 
COT: 
CTEN: 
YLI: 
CLU: 
NCR: 
SMP: 
PAN: 
TTT: 
MTM: 
CTHR: 
MGR: 
TMN: 
FGR: 
NHE: 
TRE: 
MAW: 
MAJ: 
CMT: 
VAL: 
ELA: 
SSL: 
BFU: 
MBE: 
ANI: 
AFM: 
AOR: 
AOR_1_2656174(AO090005001528)
AFV: 
ACT: 
NFI: 
PCS: 
CIM: 
CPW: 
PBL: 
URE: 
ABE: 
TVE: 
AJE: 
PNO: 
PTE: 
BZE: 
ZTR: 
PFJ: 
BCOM: 
TML: 
SPO: 
CNE: 
CNB: 
CGI: 
MBR: 
DDI: 
DPP: 
DFA: 
ACAN: 
TPS: 
PIF: 
NGD: 
EHX: 
GTT: 
NGR: 
TVA: 
 » show all
Taxonomy
Reference
1  [PMID:7287707]
  Authors
Plummer TH Jr, Tarentino AL.
  Title
Facile cleavage of complex oligosaccharides from glycopeptides by almond emulsin peptide: N-glycosidase.
  Journal
J. Biol. Chem. 256 (1981) 10243-6.
Reference
2  [PMID:901470]
  Authors
Takahashi N.
  Title
Demonstration of a new amidase acting on glycopeptides.
  Journal
Biochem. Biophys. Res. Commun. 76 (1977) 1194-201.
Reference
3  [PMID:738997]
  Authors
Takahashi N, Nishibe H.
  Title
Some characteristics of a new glycopeptidase acting on aspartylglycosylamine linkages.
  Journal
J. Biochem. (Tokyo). 84 (1978) 1467-73.
Reference
4  [PMID:4063349]
  Authors
Tarentino AL, Gomez CM, Plummer TH Jr.
  Title
Deglycosylation of asparagine-linked glycans by peptide:N-glycosidase F.
  Journal
Biochemistry. 24 (1985) 4665-71.
  Organism
Flavobacterium meningosepticum
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
83534-39-8

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