N-carbamoyl-D-amino-acid hydrolase;
D-N-carbamoylase;
N-carbamoylase (ambiguous);
N-carbamoyl-D-amino acid hydrolase

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides

N-carbamoyl-D-amino-acid amidohydrolase

an N-carbamoyl-D-amino acid + H2O = a D-amino acid + NH3 + CO2 [RN:R02190]

R02190

N-carbamoyl-D-amino acid [CPD:C05226];
H2O [CPD:C00001]

This enzyme, along with EC 3.5.1.87 (N-carbamoyl-L-amino-acid hydrolase), EC 5.1.99.5 (hydantoin racemase) and hydantoinase, forms part of the reaction cascade known as the "hydantoinase process", which allows the total conversion of D,L-5-monosubstituted hydantoins into optically pure D- or L-amino acids [2]. It has strict stereospecificity for N-carbamoyl-D-amino acids and does not act upon the corresponding L-amino acids or on the N-formyl amino acids, N-carbamoyl-sarcosine, -citrulline, -allantoin and -ureidopropanoate, which are substrates for other amidohydrolases.

1  [PMID:8462543]

Ogawa J, Shimizu S, Yamada H.

N-carbamoyl-D-amino acid amidohydrolase from Comamonas sp. E222c purification and characterization.

Eur. J. Biochem. 212 (1993) 685-91.

Comamonas sp.

2  [PMID:11849938]

Altenbuchner J, Siemann-Herzberg M, Syldatk C

Hydantoinases and related enzymes as biocatalysts for the synthesis of unnatural chiral amino acids.

Curr. Opin. Biotechnol. 12 (2001) 559-63.