KEGG   ENZYME: 3.6.1.59Help
Entry
EC 3.6.1.59                 Enzyme                                 

Name
5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] diphosphatase;
DcpS;
m7GpppX pyrophosphatase;
m7GpppN m7GMP phosphohydrolase;
m7GpppX diphosphatase;
m7G5'ppp5'N m7GMP phosphohydrolase
Class
Hydrolases;
Acting on acid anhydrides;
In phosphorus-containing anhydrides
BRITE hierarchy
Sysname
5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] N7-methylguanosine 5'-phosphate phosphohydrolase
Reaction(IUBMB)
a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] + H2O = N7-methylguanosine 5'-phosphate + a 5'-diphospho-[mRNA]
Reaction(KEGG)
(other) R04368
Show
Substrate
5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] [CPD:C02339];
H2O [CPD:C00001]
Product
N7-methylguanosine 5'-phosphate;
5'-diphospho-[mRNA]
Comment
The enzyme removes (decaps) the N7-methylguanosine 5-phosphate cap from an mRNA degraded to a maximal length of 10 nucleotides [3,6]. Decapping is an important process in the control of eukaryotic mRNA degradation. The enzyme functions to clear the cell of cap structure following decay of the RNA body [2]. The nematode enzyme can also decap triply methylated substrates, 5'-(N2,N2,N7-trimethyl 5'-triphosphoguanosine)-[mRNA] [4].
History
EC 3.6.1.59 created 2012, modified 2013
Reference
1  [PMID:16963086]
  Authors
Malys N, McCarthy JE
  Title
Dcs2, a novel stress-induced modulator of m7GpppX pyrophosphatase activity that locates to P bodies.
  Journal
J. Mol. Biol. 363 (2006) 370-82.
  Organism
Saccharomyces cerevisiae [GN:sce]
  Sequence
[sce:YLR270W]
Reference
2  [PMID:18441014]
  Authors
Liu SW, Rajagopal V, Patel SS, Kiledjian M
  Title
Mechanistic and kinetic analysis of the DcpS scavenger decapping enzyme.
  Journal
J. Biol. Chem. 283 (2008) 16427-36.
Reference
3  [PMID:12198172]
  Authors
Liu H, Rodgers ND, Jiao X, Kiledjian M
  Title
The scavenger mRNA decapping enzyme DcpS is a member of the HIT family of pyrophosphatases.
  Journal
EMBO. J. 21 (2002) 4699-708.
  Organism
Homo sapiens [GN:hsa], Saccharomyces cerevisiae [GN:sce]
  Sequence
[hsa:28960] [sce:YLR270W]
Reference
4  [PMID:14523240]
  Authors
van Dijk E, Le Hir H, Seraphin B
  Title
DcpS can act in the 5'-3' mRNA decay pathway in addition to the 3'-5' pathway.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 100 (2003) 12081-6.
  Organism
Homo sapiens [GN:hsa], Saccharomyces cerevisiae [GN:sce]
  Sequence
[hsa:28960] [sce:YLR270W]
Reference
5  [PMID:15769464]
  Authors
Chen N, Walsh MA, Liu Y, Parker R, Song H
  Title
Crystal structures of human DcpS in ligand-free and m7GDP-bound forms suggest a dynamic mechanism for scavenger mRNA decapping.
  Journal
J. Mol. Biol. 347 (2005) 707-18.
  Organism
Homo sapiens [GN:hsa]
  Sequence
[hsa:28960]
Reference
6  [PMID:15383679]
  Authors
Cohen LS, Mikhli C, Friedman C, Jankowska-Anyszka M, Stepinski J, Darzynkiewicz E, Davis RE
  Title
Nematode m7GpppG and m3(2,2,7)GpppG decapping: activities in Ascaris embryos and  characterization of C. elegans scavenger DcpS.
  Journal
RNA. 10 (2004) 1609-24.
  Organism
Ascaris suum, Caenorhabditis elegans [GN:cel]
  Sequence
Reference
7  [PMID:22985415]
  Authors
Wypijewska A, Bojarska E, Lukaszewicz M, Stepinski J, Jemielity J, Davis RE, Darzynkiewicz E
  Title
7-methylguanosine diphosphate (m(7)GDP) is not hydrolyzed but strongly bound by decapping scavenger (DcpS) enzymes and potently inhibits their activity.
  Journal
Biochemistry. 51 (2012) 8003-13.
  Organism
Ascaris suum, Caenorhabditis elegans [GN:cel], Homo sapiens [GN:hsa], Saccharomyces cerevisiae [GN:sce]
  Sequence
[up:D3K0N9] [cel:CELE_Y113G7A.9] [hsa:28960] [sce:YLR270W]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

DBGET integrated database retrieval system