KEGG   ENZYME: 4.1.99.14Help
Entry
EC 4.1.99.14                Enzyme                                 

Name
spore photoproduct lyase;
SAM;
SP lyase;
SPL;
SplB;
SplG
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
BRITE hierarchy
Sysname
spore photoproduct pyrimidine-lyase
Reaction(IUBMB)
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine (in double-helical DNA) = thymidylyl-(3'->5')-thymidylate (in double-helical DNA)
Substrate
(5R)-5,6-dihydro-5-(thymidin-7-yl)thymidine (in double-helical DNA)
Product
thymidylyl-(3'->5')-thymidylate (in double-helical DNA)
Comment
This enzyme is a member of the 'AdoMet radical' (radical SAM) family. The enzyme binds a [4Fe-4S] cluster. The cluster is coordinated by 3 cysteines and an exchangeable SAM molecule [3]. The 5'-deoxy-adenosine radical formed after electron transfer from the [4Fe-4S] cluster to the S-adenosyl-L-methionine, initiates the repair by abstracting the C-6 hydrogen of the spore photoproduct lesion. During the second part of the repair process the SAM molecule is regenerated [3].
History
EC 4.1.99.14 created 2009, modified 2010
Orthology
K03716  
spore photoproduct lyase
Genes
MMT: 
MAH: 
SUA: 
SKU: 
CHA: 
ANT: 
SBA: 
NIS: 
NAM: 
DDN: 
DMA: 
DSA: 
DAS: 
DHY: 
DPI: 
DBA: 
DRT: 
BMX: 
DAK: 
DPR: 
DSF: 
DOL: 
DAL: 
DAT: 
DTO: 
DAO: 
DTI: 
HMR: 
DAV: 
RFE: 
RRI: 
RRJ: 
RRA: 
RRC: 
RRH: 
RRB: 
RRN: 
RRP: 
RPK: 
RHE: 
RJA: 
RSV: 
RSW: 
RPP: 
RRE: 
MEX: 
MEA: 
MDI: 
MCH: 
MRD: 
MET: 
MPO: 
BSU: 
BSU13930(splB)
BSR: 
I33_1571(splB)
BSL: 
BSH: 
BSY: 
BSS: 
BST: 
GYO_1727(splB)
BSO: 
BSN: 
BSQ: 
BSUB: 
BSX: 
C663_1436(spIB)
BSP: 
BLI: 
BL03571(splB)
BLD: 
BLi01602(splB)
BLH: 
BAO: 
BAMF_1470(splB)
BAY: 
BAQ: 
BYA: 
BAMP: 
BAML: 
BAMA: 
RBAU_1353(splB)
BAMN: 
BASU_1332(splB)
BAMB: 
BAMT: 
BAZ: 
BQL: 
LL3_01497(splB)
BXH: 
BQY: 
MUS_1476(splB)
BAMI: 
BAMC: 
BAMF: 
BAE: 
BHA: 
BH2808(splB)
BAN: 
BA_4429(splB)
BAR: 
GBAA_4429(splB)
BAT: 
BAH: 
BAI: 
BAA_4445(splB)
BAX: 
BANT: 
BANR: 
BANS: 
BAL: 
BCE: 
BCA: 
BCE_4278(splB)
BCZ: 
BCZK3959(splB)
BCR: 
BCB: 
BCU: 
BCG: 
BCQ: 
BCQ_3992(splB)
BCX: 
BCA_4313(splB)
BNC: 
BCF: 
BCER: 
BTK: 
BTL: 
BALH_3810(splB)
BTB: 
BTT: 
BTC: 
BTF: 
BTM: 
BTG: 
BTI: 
BTN: 
BTHT: 
BTHU: 
BWE: 
BTY: 
BCL: 
ABC2488(splB)
BPU: 
BPUM_1283(splB)
BPUM: 
BPF: 
BMQ: 
BMQ_2226(splB)
BMD: 
BMD_2184(splB)
BMH: 
BCO: 
BJS: 
BACI: 
BIF: 
OIH: 
GTN: 
GYC: 
GYA: 
GCT: 
GGH: 
GJF: 
AFL: 
Aflv_0922(splB)
LSP: 
LGY: 
HHD: 
BBE: 
PJD: 
GYM: 
PPY: 
PPM: 
PPO: 
PPM_3056(splB)
PPOL: 
PPQ: 
PMS: 
PMQ: 
PMW: 
PTA: 
PLV: 
PSAB: 
SIV: 
CAC: 
CAE: 
CAY: 
CPE: 
CPE0724(splB)
CPF: 
CPF_0720(splB)
CTC: 
CTET: 
CNO: 
CBO: 
CBO0216(spl)
CBA: 
CLB_0257(splB)
CBH: 
CLC_0272(splB)
CBY: 
CLM_0266(splB)
CBL: 
CLK_3398(splB)
CBK: 
CBB: 
CLD_0559(splB)
CBI: 
CLJ_B0264(splB)
CBN: 
CBT: 
CBF: 
CLI_0281(splB)
CBM: 
CBJ: 
CBE: 
CKL: 
CKR: 
CCE: 
CLJ: 
CLB: 
CSR: 
CAH: 
AMT: 
AOE: 
CSS: 
CSD: 
EHA: 
CLE: 
RHO: 
CPY: 
CSH: 
CAD: 
CDF: 
CDC: 
CDL: 
CDG: 
DSY: 
DHD: 
DDL: 
DRM: 
DAE: 
DCA: 
DKU: 
DRU: 
DGI: 
PTH: 
PTH_0212(SplB) PTH_2267(SplB)
DAU: 
TJR: 
SGY: 
DOR: 
DAI: 
DMI: 
DED: 
DEC: 
DRS: 
HMO: 
HM1_2342(spIB)
ERE: 
ERT: 
ERA: 
ELM: 
OVA: 
TTE: 
TTE2677(SplB)
TEX: 
THX: 
TPD: 
TIT: 
TMT: 
TBO: 
TWI: 
MTA: 
TPZ: 
CSC: 
ATE: 
COB: 
CHD: 
COW: 
CKI: 
CKN: 
CLC: 
TOC: 
TTM: 
TTO: 
TXY: 
TSH: 
NTH: 
HOR: 
AAR: 
HHL: 
KFL: 
GOB: 
MMAR: 
KRA: 
AMS: 
ACTN: 
AFS: 
WCH: 
SNG: 
SSM: 
STA: 
STQ: 
SFC: 
SLR: 
SBU: 
SGP: 
LIL: 
LIE: 
LIC: 
LIC10195(splB)
LBJ: 
LBJ_0162(splB)
LBL: 
LBL_2921(splB)
LBI: 
LBF: 
LBF_0608(splB)
TPX: 
ACM: 
RSD: 
FNU: 
FNC: 
FUS: 
STR: 
PHM: 
CYN: 
CYJ: 
GLP: 
CMP: 
CEP: 
MIC: 
NPU: 
RIV: 
CTHE: 
SCS: 
SLI: 
FAE: 
TRA: 
PMO: 
DDF: 
DAP: 
CNI: 
FSI: 
DTH: 
DTU: 
TID: 
TOP: 
SRB: 
FPL: 
 » show all
Taxonomy
Reference
1  [PMID:16829676]
  Authors
Chandor A, Berteau O, Douki T, Gasparutto D, Sanakis Y, Ollagnier-de-Choudens S, Atta M, Fontecave M
  Title
Dinucleotide spore photoproduct, a minimal substrate of the DNA repair spore photoproduct lyase enzyme from Bacillus subtilis.
  Journal
J. Biol. Chem. 281 (2006) 26922-31.
  Organism
Bacillus subtilis
  Sequence
[bsu:BSU13930]
Reference
2  [PMID:16968710]
  Authors
Pieck JC, Hennecke U, Pierik AJ, Friedel MG, Carell T
  Title
Characterization of a new thermophilic spore photoproduct lyase from Geobacillus  stearothermophilus (SplG) with defined lesion containing DNA substrates.
  Journal
J. Biol. Chem. 281 (2006) 36317-26.
  Organism
Geobacillus stearothermophilus
Reference
3  [PMID:16829680]
  Authors
Buis JM, Cheek J, Kalliri E, Broderick JB
  Title
Characterization of an active spore photoproduct lyase, a DNA repair enzyme in the radical S-adenosylmethionine superfamily.
  Journal
J. Biol. Chem. 281 (2006) 25994-6003.
  Organism
Bacillus subtilis
  Sequence
[bsu:BSU13930]
Reference
4  [PMID:19012397]
  Authors
Mantel C, Chandor A, Gasparutto D, Douki T, Atta M, Fontecave M, Bayle PA, Mouesca JM, Bardet M
  Title
Combined NMR and DFT studies for the absolute configuration elucidation of the spore photoproduct, a UV-induced DNA lesion.
  Journal
J. Am. Chem. Soc. 130 (2008) 16978-84.
Reference
5  [PMID:20405152]
  Authors
Silver SC, Chandra T, Zilinskas E, Ghose S, Broderick WE, Broderick JB
  Title
Complete stereospecific repair of a synthetic dinucleotide spore photoproduct by  spore photoproduct lyase.
  Journal
J. Biol. Inorg. Chem. 15 (2010) 943-55.
  Organism
Clostridium acetobutylicum
  Sequence
[cac:CA_C0699]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
37290-70-3

DBGET integrated database retrieval system