KEGG   ENZYME: 4.1.99.16Help
Entry
EC 4.1.99.16                Enzyme                                 

Name
geosmin synthase
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
BRITE hierarchy
Sysname
germacradienol geosmin-lyase (acetone forming)
Reaction(IUBMB)
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O = (-)-geosmin + acetone [RN:R09487]
Reaction(KEGG)
R09487;
(other) R08543
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Substrate
(1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol [CPD:C16143];
H2O [CPD:C00001]
Product
(-)-geosmin [CPD:C16286];
acetone [CPD:C00207]
Comment
Requires Mg2+. Geosmin is the cause of the characteristic smell of moist soil. It is a bifunctional enzyme. The N-terminal part of the enzyme is EC 4.2.3.22, germacradienol synthase, and forms germacradienol from FPP. The C-terminal part of the enzyme catalyses the conversion of germacradienol to geosmin via (1S,4aS,8aS)-1,4a-dimethyl-1,2,3,4,4a,5,6,8a-octahydronaphthalene.
History
EC 4.1.99.16 created 2011
Pathway
Sesquiterpenoid and triterpenoid biosynthesis
Biosynthesis of secondary metabolites
Orthology
K10187  
germacradienol/geosmin synthase
Genes
MXA: 
MFU: 
MSD: 
CCX: 
SUR: 
STAUR_1114(geoA2)
SCL: 
SCU: 
HOH: 
NBR: 
SCO: 
SCO6073(SC9B1.20)
SMA: 
SAV_2163(geoA)
SGR: 
SGR_6839(geoA)
SCB: 
SSX: 
SVL: 
SCT: 
SCY: 
SFA: 
SBH: 
SHY: 
SHO: 
SVE: 
SDV: 
SALB: 
STRP: 
SFI: 
SCI: 
SRC: 
SALU: 
SLV: 
SGU: 
KSK: 
KSE_46080(geoA)
KFL: 
SRO: 
FRA: 
FRE: 
FRI: 
FAL: 
SEN: 
AMD: 
AMN: 
AMM: 
AMZ: 
AOI: 
AJA: 
AMQ: 
AMI: 
SESP: 
BN6_11180(geoA)
KAL: 
CAI: 
SNA: 
NPU: 
CSG: 
CALO: 
 » show all
Taxonomy
Reference
1  [PMID:16787064]
  Authors
Jiang J, He X, Cane DE.
  Title
Geosmin biosynthesis. Streptomyces coelicolor germacradienol/germacrene D synthase converts farnesyl diphosphate to geosmin.
  Journal
J. Am. Chem. Soc. 128 (2006) 8128-9.
  Sequence
[sco:SCO6073]
Reference
2  [PMID:17080683]
  Authors
Cane DE, He X, Kobayashi S, Omura S, Ikeda H
  Title
Geosmin biosynthesis in Streptomyces avermitilis. Molecular cloning, expression,  and mechanistic study of the germacradienol/geosmin synthase.
  Journal
J. Antibiot. (Tokyo). 59 (2006) 471-9.
Reference
3  [PMID:17873868]
  Authors
Jiang J, He X, Cane DE
  Title
Biosynthesis of the earthy odorant geosmin by a bifunctional Streptomyces coelicolor enzyme.
  Journal
Nat. Chem. Biol. 3 (2007) 711-5.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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