KEGG   ENZYME: 4.1.99.2Help
Entry
EC 4.1.99.2                 Enzyme                                 

Name
tyrosine phenol-lyase;
beta-tyrosinase;
L-tyrosine phenol-lyase (deaminating)
Class
Lyases;
Carbon-carbon lyases;
Other carbon-carbon lyases
BRITE hierarchy
Sysname
L-tyrosine phenol-lyase (deaminating; pyruvate-forming)
Reaction(IUBMB)
L-tyrosine + H2O = phenol + pyruvate + NH3 (overall reaction) [RN:R00728];
(1a) L-tyrosine = phenol + 2-aminoprop-2-enoate;
(1b) 2-aminoprop-2-enoate = 2-iminopropanoate (spontaneous);
(1c) 2-iminopropanoate + H2O = pyruvate + NH3 (spontaneous)
Reaction(KEGG)
Substrate
L-tyrosine [CPD:C00082];
H2O [CPD:C00001];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Product
phenol [CPD:C00146];
pyruvate [CPD:C00022];
NH3 [CPD:C00014];
2-aminoprop-2-enoate [CPD:C02218];
2-iminopropanoate [CPD:C20904]
Comment
A pyridoxal-phosphate protein. The enzyme cleaves a carbon-carbon bond, releasing phenol and an unstable enamine product that tautomerizes to an imine form, which undergoes a hydrolytic deamination to form pyruvate and ammonia. The latter reaction, which can occur spontaneously, can also be catalysed by EC 3.5.99.10, 2-iminobutanoate/2-iminopropanoate deaminase. The enzyme also slowly catalyses similar reactions with D-tyrosine, S-methyl-L-cysteine, L-cysteine, L-serine and D-serine.
History
EC 4.1.99.2 created 1972
Pathway
Tyrosine metabolism
Orthology
K01668  
tyrosine phenol-lyase
Genes
CKO: 
CFD: 
PAM: 
PLF: 
PAJ: 
PAQ: 
MMK: 
HPR: 
PMU: 
PMV: 
PUL: 
PMP: 
PMUL: 
AAP: 
AAT: 
AAO: 
AAN: 
AAH: 
DNO: 
CDN: 
PSF: 
PSE_0962(tnaA)
CTC: 
CTET: 
CSH: 
STH: 
DSY: 
DHD: 
DOR: 
TEP: 
TAE: 
NTH: 
HPK: 
PUF: 
PFT: 
SALU: 
ICA: 
AOI: 
AJA: 
ALU: 
TDE: 
TDE1118(tpl)
TPED: 
TPK: 
FNU: 
FNC: 
FNT: 
FUS: 
FNE: 
CMP: 
NPU: 
NOP: 
MRO: 
RRS: 
RCA: 
CAU: 
CAG: 
CHL: 
OPR: 
 » show all
Taxonomy
Reference
1  [PMID:4908868]
  Authors
Kumagai H, Yamada H, Matsui H, Ohkishi H, Ogata K.
  Title
Tyrosine phenol lyase. I. Purification, crystallization, and properties.
  Journal
J. Biol. Chem. 245 (1970) 1767-72.
Reference
2  [PMID:4908869]
  Authors
Kumagai H, Yamada H, Matsui H, Ohkishi H, Ogata K.
  Title
Tyrosine phenol lyase. II. Cofactor requrements.
  Journal
J. Biol. Chem. 245 (1970) 1773-7.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9059-31-8

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