KEGG   ENZYME: 4.2.1.107Help
Entry
EC 4.2.1.107                Enzyme                                 

Name
3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA hydratase;
46 kDa hydratase 2;
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA hydro-lyase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA hydro-lyase [(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA-forming]
Reaction(IUBMB)
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA = (24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA + H2O [RN:R04813]
Reaction(KEGG)
Substrate
(24R,25R)-3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestanoyl-CoA
Product
(24E)-3alpha,7alpha,12alpha-trihydroxy-5beta-cholest-24-enoyl-CoA;
H2O [CPD:C00001]
Comment
This enzyme forms part of the rat peroxisomal multifunctional enzyme perMFE-2, which also exhibits a dehydrogenase activity. The enzyme is involved in the beta-oxidation of the cholesterol side chain in the cholic-acid-biosynthesis pathway.
History
EC 4.2.1.107 created 2005
Pathway
Primary bile acid biosynthesis
Metabolic pathways
Orthology
K08078  
3a,7a,12a-trihydroxy-5b-cholest-24-enoyl-CoA hydratase
K12405  
3-hydroxyacyl-CoA dehydrogenase / 3a,7a,12a-trihydroxy-5b-cholest-24-enoyl-CoA hydratase
Genes
HSA: 
3295(HSD17B4)
PTR: 
462019(HSD17B4)
PPS: 
100981028(HSD17B4)
GGO: 
PON: 
100448539(HSD17B4)
MCC: 
698369(HSD17B4)
MCF: 
102123675(HSD17B4)
MMU: 
15488(Hsd17b4)
RNO: 
79244(Hsd17b4)
CGE: 
103161104(Hsd17b4)
HGL: 
101713520(Hsd17b4)
TUP: 
102468828(HSD17B4)
CFA: 
474630(HSD17B4)
AML: 
100471749(HSD17B4)
FCA: 
101091840(HSD17B4)
PTG: 
102968873(HSD17B4)
BTA: 
493643(HSD17B4)
BOM: 
102268517(HSD17B4)
PHD: 
102329357(HSD17B4)
CHX: 
102172142(HSD17B4)
OAS: 
101114519(HSD17B4)
SSC: 
397574(HSD17B4)
CFR: 
102508317(HSD17B4)
BACU: 
LVE: 
103084787(HSD17B4)
ECB: 
100034046(HSD17B4)
MYB: 
102240475(HSD17B4)
MYD: 
102767159(HSD17B4)
PALE: 
102887000(HSD17B4)
MDO: 
100028098(HSD17B4)
SHR: 
100932771(HSD17B4)
OAA: 
100081754(HSD17B4)
GGA: 
395785(HSD17B4)
MGP: 
TGU: 
100218226(HSD17B4)
FAB: 
101807233(HSD17B4)
PHI: 
102109547(HSD17B4)
APLA: 
101791118(HSD17B4)
FPG: 
101917400(HSD17B4)
FCH: 
102058406(HSD17B4)
CLV: 
102095771(HSD17B4)
ASN: 
102371766(HSD17B4)
AMJ: 
102571005(HSD17B4)
PSS: 
102451304(HSD17B4)
CMY: 
102933522(HSD17B4)
ACS: 
100558484(hsd17b4)
PBI: 
103063870(HSD17B4)
XLA: 
444492(hsd17b4)
XTR: 
613082(hsd17b4)
DRE: 
393105(hsd17b4)
TRU: 
MZE: 
OLA: 
XMA: 
LCM: 
102356548(HSD17B4)
CMK: 
103181119(hsd17b4)
BFO: 
CIN: 
100187421(hsd17b4)
SPU: 
581580(hsd17b4)
DME: 
DPO: 
DAN: 
DER: 
DPE: 
DWI: 
DYA: 
DGR: 
DMO: 
DVI: 
AGA: 
AAG: 
CQU: 
AME: 
409986(GB18162)
NVI: 
TCA: 
663655(17-beta-HD4)
BMOR: 
API: 
PHU: 
ISC: 
CBR: 
CBG10899(Cbr-dhs-28) CBG13089(Cbr-maoc-1)
BMY: 
LOA: 
NVE: 
HMG: 
TAD: 
AQU: 
100640903(Hsd17b4)
MBR: 
DDI: 
DPP: 
DFA: 
DFA_02002(mfeA)
ACAN: 
TGO: 
TET: 
PIF: 
NGR: 
PRE: 
SSE: 
ACR: 
DRM: 
 » show all
Taxonomy
Reference
1  [PMID:9371691]
  Authors
Qin YM, Haapalainen AM, Conry D, Cuebas DA, Hiltunen JK, Novikov DK.
  Title
Recombinant 2-enoyl-CoA hydratase derived from rat peroxisomal multifunctional enzyme 2: role of the hydratase reaction in bile acid synthesis.
  Journal
Biochem. J. 328 ( Pt 2) (1997) 377-82.
  Organism
Rattus norvegicus
Reference
2  [PMID:8619845]
  Authors
Xu R, Cuebas DA.
  Title
The reactions catalyzed by the inducible bifunctional enzyme of rat liver peroxisomes cannot lead to the formation of bile acids.
  Journal
Biochem. Biophys. Res. Commun. 221 (1996) 271-8.
  Organism
Rattus norvegicus
Reference
3
  Authors
Kinoshita, T., Miyata, M., Ismail, S.M., Fujimoto, Y., Kakinuma, K., Kokawa, N.I. and Morisaki, M.
  Title
Synthesis and determination of stereochemistry of four diastereoisomers at the C-24 and C-25 positions of 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oic acid and cholic acid.
  Journal
Chem. Pharm. Bull. 36 (1988) 134-141.
Reference
4
  Authors
Fujimoto, Y., Kinoshita, T., Oya, I., Kakinuma, K., Ismail, S.M., Sonoda, Y., Sato, Y. and Morisaki, M.
  Title
Non-stereoselective conversion of the four diastereoisomers at the C-24 and C-25 positions of 3alpha,7alpha,12alpha,24-tetrahydroxy-5beta-cholestan-26-oic acid and cholic acid.
  Journal
Chem. Pharm. Bull. 36 (1988) 142-145.
Reference
5  [PMID:11146090]
  Authors
Kurosawa T, Sato M, Nakano H, Fujiwara M, Murai T, Yoshimura T, Hashimoto T.
  Title
Conjugation reactions catalyzed by bifunctional proteins related to beta-oxidation in bile acid biosynthesis.
  Journal
Steroids. 66 (2001) 107-14.
  Organism
Rattus norvegicus
Reference
6  [PMID:12543708]
  Authors
Russell DW.
  Title
The enzymes, regulation, and genetics of bile acid synthesis.
  Journal
Annu. Rev. Biochem. 72 (2003) 137-74.
  Organism
Homo sapiens
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
152787-68-3

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