KEGG   ENZYME: 4.2.1.70Help
Entry
EC 4.2.1.70                 Enzyme                                 

Name
pseudouridylate synthase;
pseudouridylic acid synthetase;
pseudouridine monophosphate synthetase;
5-ribosyluracil 5-phosphate synthetase;
pseudouridylate synthetase;
upsilonUMP synthetase;
uracil hydro-lyase (adding D-ribose 5-phosphate);
YeiN;
pseudouridine-5'-phosphate glycosidase
Class
Lyases;
Carbon-oxygen lyases;
Hydro-lyases
BRITE hierarchy
Sysname
uracil hydro-lyase (adding D-ribose 5-phosphate; pseudouridine-5'-phosphate-forming)
Reaction(IUBMB)
uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O [RN:R01055]
Reaction(KEGG)
Substrate
uracil [CPD:C00106];
D-ribose 5-phosphate [CPD:C00117]
Product
pseudouridine 5'-phosphate [CPD:C01168];
H2O [CPD:C00001]
Comment
The reaction it readily reversible. While the enzymes from Tetrahymena pyriformis and Agrobacterium tumefaciens produce pseudouridine 5'-phosphate the enzyme from Escherichia coli functions as a pseudouridine-5'-phosphate glycosidase in vivo [5].
History
EC 4.2.1.70 created 1978
Pathway
Pyrimidine metabolism
Orthology
K01718  
pseudouridylate synthase
Genes
DPO: 
CME: 
PIC: 
PFA: 
PKN: 
TBR: 
TCR: 
LMA: 
LIF: 
LDO: 
LBZ: 
 » show all
Taxonomy
Reference
1  [PMID:14165924]
  Authors
HEINRIKSON RL, GOLDWASSER E.
  Title
STUDIES ON THE BIOSYNTHESIS OF 5-RIBOSYLURACIL  5'-MONOPHOSPHATE IN TETRAHYMENA PYRIFORMIS.
  Journal
J. Biol. Chem. 239 (1964) 1177-87.
Reference
2  [PMID:4936431]
  Authors
Matsushita T, Davis FF.
  Title
Studies on pseudouridylic acid synthetase from various sources.
  Journal
Biochim. Biophys. Acta. 238 (1971) 165-73.
Reference
3  [PMID:4569284]
  Authors
Remsen JF, Matsushita T, Chirikjian JG, Davis FF.
  Title
Enzymatic synthesis of deoxypseudouridylic acid and a study of certain of its properties.
  Journal
Biochim. Biophys. Acta. 281 (1972) 481-7.
Reference
4  [PMID:5942965]
  Authors
Suzuki T, Hochster RM.
  Title
On the biosynthesis of pseudouridine and of pseudouridylic acid in Agrobacterium tumefaciens.
  Journal
Can. J. Biochem. 44 (1966) 259-72.
Reference
5  [PMID:18591240]
  Authors
Preumont A, Snoussi K, Stroobant V, Collet JF, Van Schaftingen E.
  Title
Molecular identification of pseudouridine-metabolizing enzymes.
  Journal
J. Biol. Chem. 283 (2008) 25238-46.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9023-35-2

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