| Entry |
|
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| Name |
pectin lyase;
pectin trans-eliminase;
endo-pectin lyase;
polymethylgalacturonic transeliminase;
pectin methyltranseliminase;
pectolyase;
PL;
PNL;
PMGL
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| Class |
Lyases;
Carbon-oxygen lyases;
Acting on polysaccharides
 |
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| Sysname |
(1->4)-6-O-methyl-alpha-D-galacturonan lyase
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| Reaction(IUBMB) |
Eliminative cleavage of (1->4)-alpha-D-galacturonan methyl ester to give oligosaccharides with 4-deoxy-6-O-methyl-alpha-D-galact-4-enuronosyl groups at their non-reducing ends
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| Comment |
Favours pectin, the methyl ester, over pectate, the anion (which is the preferred substrate of EC 4.2.2.2, pectate lyase). Demethylation progressively slows its action; it can nevertheless cleave on either side of a demethylated residue if the residue at the other end of the scissile bond is methylated.
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| Orthology |
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| Genes |
 |
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| Reference |
1 |
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| Authors |
Albersheim, P., Neukom, H. and Deuel, H. |
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| Title |
Uber die Bildung von ungesattigten Abbauprodukten durch ein pekinabbauendes Enzym. |
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| Journal |
Helv. Chim. Acta 43 (1960) 1422-1426. |
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| Reference |
|
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| Authors |
Mayans O, Scott M, Connerton I, Gravesen T, Benen J, Visser J, Pickersgill R, Jenkins J. |
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| Title |
Two crystal structures of pectin lyase A from Aspergillus reveal a pH driven conformational change and striking divergence in the substrate-binding clefts of pectin and pectate lyases. |
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| Journal |
Structure. 5 (1997) 677-89. |
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| Organism |
Aspergillus niger [GN: ang] |
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| Reference |
3 |
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| Authors |
Kester, H.C.M and Visser, J. |
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| Title |
Purification and characterization of pectin lyase B, a novel pectinolytic enzyme from Aspergillus niger. |
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| Journal |
FEMS Microbiol. Lett. 120 (1994) 63-68. |
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| Reference |
|
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| Authors |
Mutenda KE, Korner R, Christensen TM, Mikkelsen J, Roepstorff P. |
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| Title |
Application of mass spectrometry to determine the activity and specificity of pectin lyase A. |
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| Journal |
Carbohydr. Res. 337 (2002) 1217-27. |
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| Organism |
Aspergillus niger [GN: ang] |
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| Other DBs |
ExplorEnz - The Enzyme Database: IUBMB Enzyme Nomenclature: ExPASy - ENZYME nomenclature database: BRENDA, the Enzyme Database: CAS: 9033-35-6 |
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