KEGG   ENZYME: 4.2.2.5Help
Entry
EC 4.2.2.5                  Enzyme                                 

Name
chondroitin AC lyase;
chondroitinase (ambiguous);
chondroitin sulfate lyase;
chondroitin AC eliminase;
chondroitinase AC;
ChnAC
Class
Lyases;
Carbon-oxygen lyases;
Acting on polysaccharides
BRITE hierarchy
Sysname
chondroitin AC lyase
Reaction(IUBMB)
Eliminative degradation of polysaccharides containing 1,4-beta-D-hexosaminyl and 1,3-beta-D-glucuronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups
Comment
Acts on chondroitin 4-sulfate and chondroitin 6-sulfate, but less well on hyaluronate. In general, chondroitin sulfate (CS) and dermatan sulfate (DS) chains comprise a linkage region, a chain cap and a repeat region. The repeat region of CS is a repeating disaccharide of glucuronic acid (GlcA) and N-acetylgalactosamine (GalNAc) [-4)GlcA(beta1-3)GalNAc(beta1-]n, which may be O-sulfated on the C-4 and/or C-6 of GalNAc and C-2 of GlcA. GlcA residues of CS may be epimerized to iduronic acid (IdoA) forming the repeating disaccharide [-4)IdoA(alpha1-3)GalNAc(beta1-]n of DS. Both the concentrations and locations of sulfate-ester substituents vary with glucosaminoglycan source [4].
History
EC 4.2.2.5 created 1972 (EC 4.2.99.6 created 1965, part incorporated 1976)
Reference
1  [PMID:13727579]
  Authors
NAKADA HI, WOLFE JB.
  Title
Studies on the enzyme chondroitinase: product structure and ion effects.
  Journal
Arch. Biochem. Biophys. 94 (1961) 244-51.
  Organism
Proteus vulgaris
Reference
2  [PMID:11500043]
  Authors
Pojasek K, Shriver Z, Kiley P, Venkataraman G, Sasisekharan R.
  Title
Recombinant expression, purification, and kinetic characterization of chondroitinase AC and chondroitinase B from Flavobacterium heparinum.
  Journal
Biochem. Biophys. Res. Commun. 286 (2001) 343-51.
  Organism
Pedobacter heparinus
Reference
3  [PMID:9761894]
  Authors
Fethiere J, Shilton BH, Li Y, Allaire M, Laliberte M, Eggimann B, Cygler M.
  Title
Crystallization and preliminary analysis of chondroitinase AC from Flavobacterium heparinum.
  Journal
Acta. Crystallogr. D. Biol. Crystallogr. 54 ( Pt 2) (1998) 279-80.
  Organism
Pedobacter heparinus
Reference
4  [PMID:16336265]
  Authors
Huckerby TN, Nieduszynski IA, Giannopoulos M, Weeks SD, Sadler IH, Lauder RM.
  Title
Characterization of oligosaccharides from the chondroitin/dermatan sulfates. 1H-NMR and 13C-NMR studies of reduced trisaccharides and hexasaccharides.
  Journal
FEBS. J. 272 (2005) 6276-86.
  Organism
Bos taurus
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
9047-57-8

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