KEGG   ENZYME: 4.2.3.114Help
Entry
EC 4.2.3.114                Enzyme                                 

Name
gamma-terpinene synthase;
OvTPS2;
ClcTS
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
geranyl-diphosphate diphosphate-lyase (cyclizing, gamma-terpinene-forming)
Reaction(IUBMB)
geranyl diphosphate = gamma-terpinene + diphosphate [RN:R09969]
Reaction(KEGG)
Substrate
geranyl diphosphate [CPD:C00341]
Product
gamma-terpinene [CPD:C09900];
diphosphate [CPD:C00013]
Comment
Isolated from Thymus vulgaris (thyme) [1,2], Citrus limon (lemon) [3], Citrus unshiu (satsuma) [4] and Origanum vulgare (oregano) [5]. Requires Mg2+. Mn2+ less effective. The reaction involves a 1,2-hydride shift. The 5-pro-S hydrogen of geranyl diphosphate is lost. Traces of several other monoterpenoids are formed in addition to gamma-terpinene.
History
EC 4.2.3.114 created 2012
Reference
1  [PMID:1897973]
  Authors
Alonso WR, Croteau R
  Title
Purification and characterization of the monoterpene cyclase gamma-terpinene synthase from Thymus vulgaris.
  Journal
Arch. Biochem. Biophys. 286 (1991) 511-7.
Reference
2  [PMID:8460944]
  Authors
LaFever RE, Croteau R
  Title
Hydride shifts in the biosynthesis of the p-menthane monoterpenes alpha-terpinene, gamma-terpinene, and beta-phellandrene.
  Journal
Arch. Biochem. Biophys. 301 (1993) 361-6.
Reference
3  [PMID:12084056]
  Authors
Lucker J, El Tamer MK, Schwab W, Verstappen FW, van der Plas LH, Bouwmeester HJ, Verhoeven HA.
  Title
Monoterpene biosynthesis in lemon (Citrus limon). cDNA isolation and functional analysis of four monoterpene synthases.
  Journal
Eur. J. Biochem. 269 (2002) 3160-71.
  Sequence
[up:Q8L5K4]
Reference
4  [PMID:15630174]
  Authors
Suzuki Y, Sakai H, Shimada T, Omura M, Kumazawa S, Nakayama T
  Title
Characterization of gamma-terpinene synthase from Citrus unshiu (Satsuma mandarin).
  Journal
Biofactors. 21 (2004) 79-82.
Reference
5  [PMID:20419468]
  Authors
Crocoll C, Asbach J, Novak J, Gershenzon J, Degenhardt J
  Title
Terpene synthases of oregano (Origanum vulgare L.) and their roles in the pathway and regulation of terpene biosynthesis.
  Journal
Plant. Mol. Biol. 73 (2010) 587-603.
  Sequence
[up:E2E2P0]
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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