KEGG   ENZYME: 4.2.3.18Help
Entry
EC 4.2.3.18                 Enzyme                                 

Name
abieta-7,13-diene synthase;
copalyl-diphosphate diphosphate-lyase (cyclizing) (ambiguous);
abietadiene synthase (ambiguous)
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
(+)-copalyl-diphosphate diphosphate-lyase [cyclizing, abieta-7,13-diene-forming]
Reaction(IUBMB)
(+)-copalyl diphosphate = abieta-7,13-diene + diphosphate [RN:R06301]
Reaction(KEGG)
Substrate
(+)-copalyl diphosphate [CPD:C11901]
Product
abieta-7,13-diene;
diphosphate [CPD:C00013]
Comment
Part of a bifunctional enzyme involved in the biosynthesis of abietadiene. See also EC 5.5.1.12, copalyl diphosphate synthase. Requires Mg2+.
History
EC 4.2.3.18 created 2002, modified 2012
Pathway
Diterpenoid biosynthesis
Metabolic pathways
Biosynthesis of secondary metabolites
Orthology
K12927  
abietadiene synthase/copalyl diphosphate synthase
Reference
1  [PMID:11112547]
  Authors
Peters RJ, Flory JE, Jetter R, Ravn MM, Lee HJ, Coates RM, Croteau RB.
  Title
Abietadiene synthase from grand fir (Abies grandis): characterization and mechanism of action of the "pseudomature" recombinant enzyme.
  Journal
Biochemistry. 39 (2000) 15592-602.
  Sequence
[up:Q38710]
Reference
2  [PMID:11552804]
  Authors
Peters RJ, Ravn MM, Coates RM, Croteau RB.
  Title
Bifunctional abietadiene synthase: free diffusive transfer of the (+)-copalyl diphosphate intermediate between two distinct active sites.
  Journal
J. Am. Chem. Soc. 123 (2001) 8974-8.
  Sequence
[up:Q38710]
Reference
3  [PMID:11805316]
  Authors
Peters RJ, Croteau RB.
  Title
Abietadiene synthase catalysis: mutational analysis of a prenyl diphosphate ionization-initiated cyclization and rearrangement.
  Journal
Proc. Natl. Acad. Sci. U. S. A. 99 (2002) 580-4.
  Sequence
[up:Q38710]
Reference
4  [PMID:11827528]
  Authors
Peters RJ, Croteau RB.
  Title
Abietadiene synthase catalysis: conserved residues involved in protonation-initiated cyclization of geranylgeranyl diphosphate to (+)-copalyl diphosphate.
  Journal
Biochemistry. 41 (2002) 1836-42.
  Sequence
[up:Q38710]
Reference
5  [PMID:12059223]
  Authors
Ravn MM, Peters RJ, Coates RM, Croteau R.
  Title
Mechanism of abietadiene synthase catalysis: stereochemistry and stabilization of the cryptic pimarenyl carbocation intermediates.
  Journal
J. Am. Chem. Soc. 124 (2002) 6998-7006.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
157972-08-2

DBGET integrated database retrieval system