KEGG   ENZYME: 4.2.3.27Help
Entry
EC 4.2.3.27                 Enzyme                                 

Name
isoprene synthase;
ISPC;
ISPS
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
dimethylallyl-diphosphate diphosphate-lyase (isoprene-forming)
Reaction(IUBMB)
dimethylallyl diphosphate = isoprene + diphosphate [RN:R08199]
Reaction(KEGG)
Substrate
dimethylallyl diphosphate [CPD:C00235]
Product
isoprene [CPD:C16521];
diphosphate [CPD:C00013]
Comment
Requires Mg2+ or Mn2+ for activity. This enzyme is located in the chloroplast of isoprene-emitting plants, such as poplar and aspen, and may be activitated by light-dependent changes in chloroplast pH and Mg2+ concentration [2,8].
History
EC 4.2.3.27 created 2007
Pathway
Terpenoid backbone biosynthesis
Biosynthesis of secondary metabolites
Orthology
K12742  
isoprene synthase
Reference
1  [PMID:16668590]
  Authors
Silver GM, Fall R.
  Title
Enzymatic Synthesis of Isoprene from Dimethylallyl Diphosphate in Aspen Leaf Extracts.
  Journal
Plant. Physiol. 97 (1991) 1588-1591.
Reference
2  [PMID:7768893]
  Authors
Silver GM, Fall R.
  Title
Characterization of aspen isoprene synthase, an enzyme responsible for leaf isoprene emission to the atmosphere.
  Journal
J. Biol. Chem. 270 (1995) 13010-6.
  Organism
Populus tremuloides
Reference
3  [PMID:12226383]
  Authors
Wildermuth MC, Fall R.
  Title
Light-Dependent Isoprene Emission (Characterization of a Thylakoid-Bound Isoprene Synthase in Salix discolor Chloroplasts).
  Journal
Plant. Physiol. 112 (1996) 171-182.
Reference
4
  Authors
Schnitzler, J.P., Arenz, R., Steinbrecher, R. and Lehming, A.
  Title
Characterization of an isoprene synthase from leaves of Quercus petraea.
  Journal
Bot. Acta 109 (1996) 216-221.
Reference
5  [PMID:11506373]
  Authors
Miller B, Oschinski C, Zimmer W.
  Title
First isolation of an isoprene synthase gene from poplar and successful expression of the gene in Escherichia coli.
  Journal
Planta. 213 (2001) 483-7.
  Organism
Populus tremuloides
  Sequence
[up:Q9AR86]
Reference
6  [PMID:12054742]
  Authors
Sivy TL, Shirk MC, Fall R.
  Title
Isoprene synthase activity parallels fluctuations of isoprene release during growth of Bacillus subtilis.
  Journal
Biochem. Biophys. Res. Commun. 294 (2002) 71-5.
Reference
7  [PMID:15848197]
  Authors
Sasaki K, Ohara K, Yazaki K.
  Title
Gene expression and characterization of isoprene synthase from Populus alba.
  Journal
FEBS. Lett. 579 (2005) 2514-8.
  Organism
Populus alba
  Sequence
[up:Q50L36]
Reference
8  [PMID:16052321]
  Authors
Schnitzler JP, Zimmer I, Bachl A, Arend M, Fromm J, Fischbach RJ.
  Title
Biochemical properties of isoprene synthase in poplar (Populus x canescens).
  Journal
Planta. 222 (2005) 777-86.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 
CAS: 
139172-14-8

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