KEGG   ENZYME: 4.2.3.61Help
Entry
EC 4.2.3.61                 Enzyme                                 

Name
5-epiaristolochene synthase;
5-epi-aristolochene synthase;
tobacco epiaristolochene synthase;
farnesyl pyrophosphate cyclase (ambiguous);
EAS;
TEAS
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
(2E,6E)-farnesyl-diphosphate diphosphate-lyase [(+)-5-epiaristolochene-forming]
Reaction(IUBMB)
(2E,6E)-farnesyl diphosphate = (+)-5-epiaristolochene + diphosphate [RN:R09574]
Reaction(KEGG)
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448]
Product
(+)-5-epiaristolochene [CPD:C19708];
diphosphate [CPD:C00013]
Comment
Initial cyclization gives (+)-germacrene A in an enzyme bound form which is not released to the medium.
History
EC 4.2.3.61 created 2011
Pathway
Sesquiterpenoid and triterpenoid biosynthesis
Orthology
K15804  
5-epiaristolochene synthase
Reference
1  [PMID:7986100]
  Authors
Back K, Yin S, Chappell J
  Title
Expression of a plant sesquiterpene cyclase gene in Escherichia coli.
  Journal
Arch. Biochem. Biophys. 315 (1994) 527-32.
Reference
2  [PMID:9295271]
  Authors
Starks CM, Back K, Chappell J, Noel JP
  Title
Structural basis for cyclic terpene biosynthesis by tobacco 5-epi-aristolochene synthase.
  Journal
Science. 277 (1997) 1815-20.
  Sequence
[up:Q40577]
Reference
3  [PMID:9816674]
  Authors
Back K, He S, Kim KU, Shin DH
  Title
Cloning and bacterial expression of sesquiterpene cyclase, a key branch point enzyme for the synthesis of sesquiterpenoid phytoalexin capsidiol in UV-challenged leaves of Capsicum annuum.
  Journal
Plant. Cell. Physiol. 39 (1998) 899-904.
  Sequence
[up:O65323]
Reference
4
  Authors
Rising, K.A., Starks, C.M., Noel, J.P. and Chappell, J.
  Title
Demonstration of germacrene A as an intermediate in 5-epi-aristolochene synthase catalysis.
  Journal
J. Am. Chem. Soc. 122 (2000) 1861-1866.
Reference
5  [PMID:12009313]
  Authors
Bohlmann J, Stauber EJ, Krock B, Oldham NJ, Gershenzon J, Baldwin IT
  Title
Gene expression of 5-epi-aristolochene synthase and formation of capsidiol in roots of Nicotiana attenuata and N. sylvestris.
  Journal
Phytochemistry. 60 (2002) 109-16.
  Sequence
[up:Q84LF1]
Reference
6  [PMID:16375847]
  Authors
O'Maille PE, Chappell J, Noel JP
  Title
Biosynthetic potential of sesquiterpene synthases: alternative products of tobacco 5-epi-aristolochene synthase.
  Journal
Arch. Biochem. Biophys. 448 (2006) 73-82.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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