KEGG   ENZYME: 4.2.3.87Help
Entry
EC 4.2.3.87                 Enzyme                                 

Name
alpha-guaiene synthase;
PatTps177 (gene name)
Class
Lyases;
Carbon-oxygen lyases;
Acting on phosphates
BRITE hierarchy
Sysname
(2Z,6E)-farnesyl-diphosphate diphosphate-lyase (cyclizing, alpha-guaiene-forming)
Reaction(IUBMB)
(2E,6E)-farnesyl diphosphate = alpha-guaiene + diphosphate [RN:R09887]
Reaction(KEGG)
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448]
Product
alpha-guaiene [CPD:C20161];
diphosphate [CPD:C00013]
Comment
Requires Mg2+. The enzyme from Pogostemon cablin gives 13% alpha-guaiene as well as 37% (-)-patchoulol (see EC 4.2.3.70), 13% delta-guaiene (see EC 4.2.3.93), and traces of at least ten other sesquiterpenoids [1]. In Aquilaria crassna three clones of the enzyme gave about 80% delta-guaiene and 20% alpha-guaiene, with traces of alpha-humulene. A fourth clone gave 54% delta-guaiene and 45% alpha-guaiene [2].
History
EC 4.2.3.87 created 2011
Reference
1  [PMID:16970904]
  Authors
Deguerry F, Pastore L, Wu S, Clark A, Chappell J, Schalk M
  Title
The diverse sesquiterpene profile of patchouli, Pogostemon cablin, is correlated  with a limited number of sesquiterpene synthases.
  Journal
Arch. Biochem. Biophys. 454 (2006) 123-36.
  Organism
Pogostemon cablin
  Sequence
[up:Q49SP3]
Reference
2  [PMID:20959422]
  Authors
Kumeta Y, Ito M
  Title
Characterization of delta-guaiene synthases from cultured cells of Aquilaria, responsible for the formation of the sesquiterpenes in agarwood.
  Journal
Plant. Physiol. 154 (2010) 1998-2007.
  Organism
Aquilaria crassna
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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