KEGG   ENZYME: 4.3.1.28Help
Entry
EC 4.3.1.28                 Enzyme                                 

Name
L-lysine cyclodeaminase;
rapL (gene name);
fkbL (gene name);
tubZ (gene name);
visC (gene name)
Class
Lyases;
Carbon-nitrogen lyases;
Ammonia-lyases
BRITE hierarchy
Sysname
L-lysine ammonia-lyase (cyclizing; ammonia-forming)
Reaction(IUBMB)
L-lysine = L-pipecolate + NH3 [RN:R10277]
Reaction(KEGG)
Substrate
L-lysine [CPD:C00047]
Product
L-pipecolate [CPD:C00408];
NH3 [CPD:C00014]
Comment
Requires bound NAD+. The enzyme produces the non-proteinogenic amino acid L-pipecolate, which is incorporated into multiple secondary metabolite products, including rapamycin, tobulysin, virginiamycin and pristinamycin.
History
EC 4.3.1.28 created 2012
Reference
1  [PMID:9473033]
  Authors
Khaw LE, Bohm GA, Metcalfe S, Staunton J, Leadlay PF
  Title
Mutational biosynthesis of novel rapamycins by a strain of Streptomyces hygroscopicus NRRL 5491 disrupted in rapL, encoding a putative lysine cyclodeaminase.
  Journal
J. Bacteriol. 180 (1998) 809-14.
  Sequence
[up:Q54304]
Reference
2  [PMID:16536560]
  Authors
Gatto GJ Jr, Boyne MT 2nd, Kelleher NL, Walsh CT
  Title
Biosynthesis of pipecolic acid by RapL, a lysine cyclodeaminase encoded in the rapamycin gene cluster.
  Journal
J. Am. Chem. Soc. 128 (2006) 3838-47.
  Sequence
[up:Q54304]
Reference
3  [PMID:17291665]
  Authors
Tsotsou GE, Barbirato F
  Title
Biochemical characterisation of recombinant Streptomyces pristinaespiralis L-lysine cyclodeaminase.
  Journal
Biochimie. 89 (2007) 591-604.
Other DBs
ExplorEnz - The Enzyme Database: 
IUBMB Enzyme Nomenclature: 
ExPASy - ENZYME nomenclature database: 
BRENDA, the Enzyme Database: 

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